A common feature of tumor cells is the aberrant expression of ion channels on their plasma membrane. The molecular mechanisms regulating ion channel expression in cancer cells are still poorly known. K+ channels that belong to the human ether-a-go-go-related gene 1 (herg1) family are frequently misexpressed in cancer cells compared to their healthy counterparts. We describe here a posttranslational mechanism for the regulation of hERG1 channel surface expression in cancer cells. This mechanism is based on the activity of hERG1 isoforms containing the USO exon. These isoforms (i) are frequently overexpressed in human cancers, (ii) are retained in the endoplasmic reticulum, and (iii) form heterotetramers with different proteins of the hERG family. (iv) The USO-containing heterotetramers are retained intracellularly and undergo ubiquitin-dependent degradation. This process results in decreased hERG1 current (IhERG1) density. We detailed such a mechanism in heterologous systems and confirmed its functioning in tumor cells that endogenously express hERG1 proteins. The silencing of USO-containing hERG1 isoforms induces a higher IhERG1 density in tumors, an effect that apparently regulates neurite outgrowth in neuroblastoma cells and apoptosis in leukemia cells.
- American Society for Microbiology
- Identification of a posttranslational mechanism for the ...
Identification of a posttranslational mechanism for the regulation of herg1 k+ channel expression and herg1 current density in tumor cells
Courtesy of American Society for Microbiology
The History of CRISPR-Cas9
The CRISPR (Clustered regularly interspaced short palindromic repeats) system was first identified in archaea as an adaptive defensive mechanism that confers resistance to foreign genetic elements. Later on, the CRISPR-Cas system was engineered into a versatile gene-editing tool enabling manipulation of protospacer adjacent motif (PAM) downstream DNA. Now, CRISPR-Cas9 has facilitated robust genome editing in virtually any organism including: human cells, rat, mice, zebra fish, bacteria, fruit flies, yeast,...
The silence of the lambs - Case study
Helicopters and aircraft In this particular area there were quite a few helicopters and aircraft and these were picked up by the Invictus. “You find these days that developers are being asked for more and more studies or evidence over issues such as environmental noise, particularly in rural locations. Some would seem almost comical but it is becoming more the norm to meet planning requirements and developers have to comply.” Due to the rural location, Louise was able to utilise the remote access and...
Reading borough council - Combating noise nuisance - case study
The Issue Commercial and domestic noise nuisance complaints. The Solution Anyone browsing noise nuisance in Reading will very quickly come across these questions on the Council’s website: Are you being driven mad by persistent loud music from a neighbour? Is someone carrying out noisy DIY work late at night or early in the morning? Is there a noisy pub or shop nearby? Are barking dogs causing sleepless nights? And their advice is loud and clear - “don’t suffer in silence” Like all...
Five questions to Henk Janssen, technical director of Watco Group
Five questions to Henk Janssen, technical director of Watco Group, water-cooling engineer with 35 years of experience under the belt on the future of water cooling. This episode on cooling towers. What attracted you to pursue a career in water cooling in the first place? Mainly it was the opportunity to travel all over the world. Then, due to a coincidence of factors, I had the opportunity to take over a small company in cooling towers, and I settled down with my young family to focus on markets in North West...
ATEX: Blower Technology And Explosion Protection
Aerzen Combines Effective Reactions With Preventive Measures As specialist in blower and compressor technology for conveying air and gaseous media, the AERZEN Ex-protection within different ATEX-zones is a part of day-to-day business. Combustible dust, spatial distribution, oxygen share, ignition source and the closed reservoir are components of a dust explosion and form the so-called explosion pentagon. COMBUSTIBLE MATERIAL Dust-air mixtures are explosive when the dust consists of combustible...