Keywords: sterilisation, collagen, Chondrosia reniformis, autoclave, gamma irradiation, ethylene oxide, denaturation, Fourier transform infrared spectroscopy, FTIR, differential scanning calorimetry, DSC, chain scission, cross linking, helical content, biomaterials, marine Demosponge
Identification of a suitable sterilisation method for collagen derived from a marine Demosponge
Collagen is widely used as a biomedical material, and its importance is likely to grow as research and understanding progresses in this field. As a biomedical material, ensuring the sterility of collagen before use as, or incorporation into, a medical device is paramount. However, common sterilisation techniques can induce changes in the physical structure and protein chemistry of collagen, potentially affecting the performance. In this preliminary study, the influence of autoclaving, gamma irradiation and ethylene oxide gas sterilisation on the denaturation temperature and helical content of the collagen was evaluated using differential scanning calorimetry and Fourier transform infrared spectroscopy. Early results indicate that all sterilisation techniques affect collagen properties but suggest that the least damaging of the techniques investigated was γ irradiation.