Keywords: desorption, heavy metals, bacterial immobilisation, microbial reactors, microbial viability, copper removal, Pseudomonas aeruginosa, environmental pollution
Immobilised microbial reactor for heavy metal pollution control
The copper removal potential of Pseudomonas aeruginosa immobilised on different adsorbent resins has been studied. Duolite S-761 was found to be the most suitable matrix for bacterial immobilisation. Column studies were conducted using virgin as well as bacteria-immobilised resin. The experimental results reveal that resin with immobilised cells as sorbent increased the column capacity by more than 100%. Sorbed copper (II) could be desorbed using citrate buffer, and the bacteria immobilised reactor could be reused for removal of copper(II) with more than 90% efficiency. Further, the immobilised microbes were found to be viable. Hydrochloric acid (0.1 N) was able to desorb almost 100% of the sorbed metal, but the viability of the cells was affected, as expected.