John Wiley & Sons, Ltd.

Indirectly suspended droplet microextraction of water‐miscible organic solvents by salting‐out effect for the determination of polycyclic aromatic hydrocarbons

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A simple and low cost method “indirectly suspended droplet microextraction of water‐miscible organic solvents (ISDME)” by salting‐out effect prior to high performance liquid chromatography and UV detection (HPLC‐UV) was used for the determination of polycyclic aromatic hydrocarbons (PAHs) in different samples. ISDME is a combination of salting‐out extraction of water‐miscible organic solvent and directly suspended droplet microextraction (DSDME). 95 µL of water‐miscible organic solvent (1‐propanol) was added to 500 µL of sample. A homogeneous solution was formed immediately. To produce a steady vortex at the top of solution, the sample was agitated at 700 rpm using a magnetic stirrer. By addition of ammonium sulfate (saturated solution) to the homogeneous solution, 1‐propanol was separated and collected at the bottom of steady vortex. Finally 20 µL of 1‐propanol was injected into HPLC‐UV. The effect of important parameters such as water‐miscible organic solvent (type and volume), type of salt and extraction time was evaluated. Under optimum conditions the method has a good linear calibration range (0.1‐300 µg/L), coefficients of determination (r2 > 0.998), low limits of detection (between 0.02 to 0.27 µg/L) and acceptable recovery (>85.0%). Environ Toxicol Chem © 2014 SETAC

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