This work investigates the role of the atypical dual tyrosine phosphatase 3 (DUSP3 or VHR) in Ionising Radiation Induced Foci (IRIF) in HeLa cells following gamma and ultraviolet C radiation. The goal is to identify possible involvements of this enzyme in DNA repair and genomic instability. Experimental approaches were based on: (a) fluorescence confocal microscopy of VHR and other proteins for determination of their expression and intracellular localisation, and (b) systems biology investigation of structural features of proteins known to be involved in DNA damage response and repair and comparison with known VHR substrates. At the IRIFs, VHR co-localises with p-H2AX and p-ATF2 after γ-ray, and with p-JNK and p-ATF2 after UVC radiation. Homology search approaches raised a list of several putative substrates for VHR based on sequence alignments. For some of these putative substrates the interaction was confocal microscopy analysis and shown to co-localised directly or indirectly (Mre11) with VHR in the IRIFs. These results shed light in the dual tyrosine phosphatases as players in DNA damage and response.
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Characterization of modified PVDF membrane by gamma irradiation for non-potable water reuse
Poly(vinylidene fluorine) (PVDF) membranes were grafted by gamma-ray irradiation and were sulfonated by sodium sulfite to modify the surface of the membranes. The characteristics of the modified PVDF membranes were evaluated by the data of Fourier transform infrared (FT-IR), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscope (FE-SEM), the contact angle of the membrane surface and the water permeability. From the results of FT-IR, XPS and FE-SEM, it was shown that the modified...
Experimental determination of dose uniformity in the CDTN gamma irradiator
This paper describes an experimental study of dose uniformity within a container used for products irradiation, in the gamma irradiation facility at Nuclear Technology Development Center (CDTN). The CDTN is a research institute of the National Nuclear Energy Commission (CNEN), Brazil. Polymethylmethacrylate (PMMA) dosimeters were used in order to determine the Dose Uniformity Ratio (DUR) inside the container. Furthermore, the effect of product density on DUR was studied. The knowledge of these parameters helps to...
Laser–assisted nuclear photoeffect re–examined
In a recent article of Dadi and Müller the modification of nuclear photoeffect due to intense laser field is discussed. The paper is criticised from the point of view of gauge invariance. The S–matrix element and the cross–section of the laser–assisted nuclear photoeffect are recalculated in a gauge invariant manner taking into account the effect of the Coulomb field of the remainder nucleus. The γ–photon energy dependence of the laser–free cross–section obtained in the plane wave and long–wavelength Coulomb–Volkov...
Performance of some handheld dosimeters used for gamma–ray ambient dose rate measurements
A study was carried out on the performance of some handheld dosimeters that are used for measuring ambient gamma dose rates. A CsI scintillation–based and two GM–based dosimeters were used to measure the absorbed dose rates in an indoor environment, inside a lead shield, on different floors of a building, in a desert environment and in a boat on the Arabian Gulf. The study showed that while some instruments provide measurements of ambient gamma dose rates, which include contributions from both terrestrial and...
Estimation of238U,Aug. 1, 2013
The first few measurements for low level gamma ray spectrometry for the delineation of U and Th along South Purulia Shear Zone (SPSZ) are reported. Estimation of 238U, 232Th and 40K concentrations in widely dispersed rock and soil samples around SPSZ has been undertaken using HPGe detector having relative efficiency of 50%. The maximum concentration among all the samples studied is observed in the sample close to the Beldih region, SPSZ. An attempt has been made to study the variation in the concentration of...