Methylibium petroleiphilum PM1 is a well-characterized environmental strain capable of complete metabolism of the fuel oxygenate methyl tert-butyl ether (MTBE). Using a molecular genetic system which we established to study MTBE metabolism by PM1, we demonstrated that the enzyme MdpA is involved in MTBE removal, based on insertional inactivation and complementation studies. MdpA is constitutively expressed at low levels but is strongly induced by MTBE. MdpA is also involved in the regulation of tert-butyl alcohol (TBA) removal under certain conditions but is not directly responsible for TBA degradation. Phylogenetic comparison of MdpA to related enzymes indicates close homology to the short-chain hydrolyzing alkane hydroxylases (AH1), a group that appears to be a distinct subfamily of the AHs. The unique, substrate-size-determining residue Thr59 distinguishes MdpA from the AH1 subfamily as well as from AlkB enzymes linked to MTBE degradation in Mycobacterium austroafricanum.
- American Society for Microbiology
- Involvement of a Novel Enzyme, Mdpa, in methyl tert-butyl ether ...
Rolling out INFERNOFUEL/METHANOL as a national fuel mixed for a greener planet
As China’s economy has grown over the past decade at a breakneck pace it has struggled to match the increasing demand for fuel for cars that its people are buying in ever greater numbers. In an effort to solve this problem the Chinese looked for an alternative liquid fuel source and settled on methanol. In less than a decade China’s methanol use in the transportation sector has grown from virtually zero to providing 8% of the country’s fuel supply, and reducing the demand of gasoline by a fairly...
Polishing of BTEX And MTBE Service Station Closure Effort - Case Study
Aservice station in Montgomery County, Pa underwent a hydro- geologic re-assessment in March 2002. The contaminants at the site included BTEX constituents; MTBE, a fuel oxygenate; Isopropyl-benzene; and Napthalene. These Contaminants of Concern (COC’s) were not degrading rapidly through natural attenuation and direct oxidation in the form of Fenton’s Reagent. Initially, several Fenton’s Reagent applications were applied through permanently installed injection points, with little effect...
Short term fish reproduction assays with methyl tertiary butyl ether (MTBE) with zebrafish and ...
This paper reports on short‐term fish reproduction assays in zebrafish and fathead minnow conducted to examine the potential for methyl tertiary butyl ether (MTBE) to cause effects on the endocrine system. Both studies were performed under Good Laboratory Practice and in accordance with OECD and USEPA test guidelines. Results of the first study demonstrated that exposure to a high test concentration (147 mg/L) of MTBE impaired reproductive output of female zebrafish, evident by a reduction in fecundity. However,...
MPPE for MTBE, BTEX, GrOs and DROs removal case study
Analytical results Germany MTBE in: 550 Out: < 2 dl, removal % = > 99.2 BTEX in: 1,693 Out: < 1 dl, removal % = >99.9 PAHs in: 49,7 Out: < 1 dl, removal % = > 98 Analytical results Agip Italy (µg/l) MTBE in: 4,860 Out: < 0.1 dl, removal% > 99.9 BTEX in: 981.8 Out: < 0.1 dl, removal%> 99.9 GROs in: 2,315 Out: < 5 dl, removal% > 99.8 DROs in: 280 Out: < 5 dl, removal% > 99 GROs: Gasoline Range Organics (C7-C10) DROs: Diesel Range Organics (C11-C27)
Advancing Green Technology case study
Challenge: A subject of considerable research in recent years, sulfate reducing bacteria have been demonstrated to be a dominant factor in the natural anaerobic biodegradation of petroleum hydrocarbons dissolved in groundwater. Antea Group has conducted extensive pilot testing at hundreds of sites across the United States that demonstrate the benefits of enhancing sulfate concentrations in groundwater to stimulate the biodegradation of petroleum contaminants under varied geological and hydrogeological settings....