To determine the suitable development period of zebrafish for accurately evaluating estrogenic activities. An enzyme‐linked immunosorbent assay (ELISA) was developed and used to detect the Vtg‐derived yolk proteins and newly produced Vtg, and 9–56 d post‐hatching (dph) was determined as the Vtg‐blank period. Juveniles in this period was found to have lower baseline Vtg level than adult males and was considered an alternative test organism for detecting environmental estrogens. This article is protected by copyright. All rights reserved
A new system for monitoring toxicity TOXcontrol® (MicroLAN BV, The Netherlands) has been used to assess the toxicity of a selection of priority or emergent compounds in the laboratory. In this study, inhibition curves and EC50 – Effective Concentration causing 50% inhibition – of selected compounds (including pesticides, pharmaceuticals, surfactants and metals commonly detected in surface or drinking waters) were determined. This new technology is based on the measurement of Vibrio fischeri...
Giardia intestinalis is a protozoan parasite, transmitted to humans and animals by the faecal–oral route, mainly through contaminated water and food. Knowledge about the distribution of this parasite in surface water in Poland is fragmentary and incomplete. Accordingly, 36 environmental water samples taken from surface water reservoirs and wells were collected in Pomerania and Warmia-Masuria provinces, Poland. The 50 L samples were filtered and subsequently analysed with three molecular detection methods: loop-medi...
Soluble microbial products, consisting of protein, carbohydrate and humics, are generally considered as the main membrane foulants during the performance of membrane bioreactors. Nitrate and nitrite have been proved to affect the determination of carbohydrate when anthrone-sulfuric acid photometric method is used. In this study, three chemical analytical methods based on photometric assay, including the standard curve method, conventional standard addition method and H-point standard addition method, were...
The aim of the study was to examine heavy metal tolerance (Cd2+, Zn2+, Ni2+ and Cu2+) of single- and mixed-species biofilms (Rhodotorula mucilaginosa and Escherichia coli) and to determine metal removal efficiency (Cd2+, Zn2+, Ni2+, Cu2+, Pb2+ and Hg2+). Metal tolerance was quantified by crystal violet assay and results were confirmed by fluorescence microscopy. Metal removal efficiency was determined by batch biosorption assay. The tolerance of the mixed-species biofilm was higher than the single-species biofilms....
Alkaline Phosphatase Enzyme Activity Measurement as a Robust on-line Monitoring Assay for Water Quality Assessment.
Zina Al-Saar 4th year Intern student Avans University of Applied Science - ATGM Department Joep Appels - Supervisor and Managing Director microLAN on line Bio-monitoring System.
Many heterotrophic microorganisms maintain their growth by producing enzymes in order to provide low molecular weight inorganic compounds. These compounds could be up taken into the cells from high molecular weight...
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