To determine the suitable development period of zebrafish for accurately evaluating estrogenic activities. An enzyme‐linked immunosorbent assay (ELISA) was developed and used to detect the Vtg‐derived yolk proteins and newly produced Vtg, and 9–56 d post‐hatching (dph) was determined as the Vtg‐blank period. Juveniles in this period was found to have lower baseline Vtg level than adult males and was considered an alternative test organism for detecting environmental estrogens. This article is protected by copyright. All rights reserved
Alkaline Phosphatase Enzyme Activity Measurement as a Robust on-line Monitoring Assay for Water Quality Assessment.
Zina Al-Saar 4th year Intern student Avans University of Applied Science - ATGM Department Joep Appels - Supervisor and Managing Director microLAN on line Bio-monitoring System.
Many heterotrophic microorganisms maintain their growth by producing enzymes in order to provide low molecular weight inorganic compounds. These compounds could be up taken into the cells from high molecular weight...
For the 2014 World Cup and the 2016 Olympic Games, Brazil has expanded its airport infrastructure. This will lead to an increase in wastewater generation from aircrafts. This wastewater is traditionally taken from the aircrafts and disposed in the public sewage collection system. However, this residual water may have a different composition than the usual sanitary sewage. Therefore, it is important to study an alternative to treat this kind of wastewater. Thus, the objective of this study was to characterize and...
Desalination of seawater is becoming an important means to address the increasing scarcity of freshwater resources in the world. Seawater has been used as drinking water in the health, food, and medical fields and various beneficial effects have been suggested, although not confirmed. Given the presence of 63 minerals and trace elements in drinking desalinated seawater (63 DSW), we evaluated their effects on the behavior of tumorigenic and nontumorigenic cells through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl...
Alkaline Phosphatase Enzyme Activity Measurement as a Robust on-line Monitoring Assay for Water Quality Assessment.
Micro organisms maintain their growth by producing enzymes in order to provide low molecular weight inorganic compounds. These compounds could be taken up into the cells from high molecular weight organic compounds which cannot be directly utilized by bacteria. Microbial enzymes are, therefore, directly related to the concentrations and ratios of limiting nutrients, and may be sensitive indicators...
The mEI, Chromocult® enterococci, and m-Enterococcus culture-based methods used to assess water quality by the detection of Enterococcus spp. were first compared in terms of sensitivity using (1) 41 different type strains of Enterococcus spp. and (2) environmental colonies identified by 16S rRNA sequencing. Then, two specific-rtPCR assays targeting Enterococcus spp. and Enterococcus faecalis/faecium were tested for their ability to confirm the identity of putative enterococcal colonies. The mEI, Chromocult®...
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