John Wiley & Sons, Ltd.

Juvenile zebrafish in the vitellogenin blank period as an alternative test organism for evaluation of estrogenic activity of chemicals

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To determine the suitable development period of zebrafish for accurately evaluating estrogenic activities. An enzyme‐linked immunosorbent assay (ELISA) was developed and used to detect the Vtg‐derived yolk proteins and newly produced Vtg, and 9–56 d post‐hatching (dph) was determined as the Vtg‐blank period. Juveniles in this period was found to have lower baseline Vtg level than adult males and was considered an alternative test organism for detecting environmental estrogens. This article is protected by copyright. All rights reserved

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