KB-1® Bioaugmentation Leading to Site Closure


Courtesy of SIREM

Problem Definition
In 2004, the site was an active manufacturing facility with plume trichloroethene (TCE) concentrations ranging from 1 to 20 milligrams per liter (mg/L), and hexavalent chromium (Cr[VI]) ranging from 0.5 to 7 mg/L. Intermediate degradation products of TCE were present in only trace concentrations in this primarily aerobic aquifer. The site source area was excavated in March 2004, to remove 800 tons of TCE-contaminated soil. Enhanced in-situ bioremediation (EISB) was implemented to remediate the remaining plume.

EISB for treatment of the TCE and Cr(VI)-impacted groundwater was implemented via a forcedgradient flow field to distribute lactate (electron donor) throughout the plume. After a two month conditioning period, strongly reducing conditions were established and 12 wells located throughout the plume were bioaugmented with approximately 3 liters each of the KB-1® culture to introduce Dehalococcoides (Dhc) bacteria.

Notable Results
Within five months of KB-1® bioaugmentation, significant enhanced reductive dechlorination was evident, resulting in accumulation of the non-toxic dechlorination product ethene. Gene-Trac Dhc testing indicated high concentrations (107 Dhc per liter of groundwater) post bioaugmentation. Within one year, Cr(VI) was reduced to concentrations below detection limits. Within three years, TCE, cis-1,2-dichloroethene and vinyl chloride concentrations in groundwater were reduced to levels below risk based cleanup criteria across the site and the Danish regulatory authority approved
a no further remedial action designation for the site.

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