amino acid analyzer Articles

  • The development of cation-exchange chromatography in free amino acids analysis

    Although it was not called a liquid chromatograph, the first such instrument was introduced in late 1959 by Beckman Instruments and was designated the M.S. Amino Acid Analyzer in honour of Moore and Stein. Inject valves had yet to be invented, the buffer switching valves were glass stopcocks and the column temperature was controlled by circulating water baths. Two positive displacement pumps ...


    By Pickering Laboratories, Inc.

  • Amino acid analysis matrix independent analysis of free amino acids

    Pickering Laboratories specializes in manufacturing of cationexchange columns and eluants for amino acid analysis. No other techniques have been shown to match the reproducibility and sensitivity of this post-column methodology. Advantages of this method, such as absence of matrix interferences, are especially important in analysis of native samples. Pickering provides several options for the ...


    By Pickering Laboratories, Inc.

  • o-Phthalaldehyde Reagent

    Introduction Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan under basic conditions. At a pH>9 and ambient temperature, reaction is generally complete within 30 seconds. The products of this reaction, 1-alkyl-2-alkylthio-substituted isoindoles, exhibit optimal excitation at 330 nm and maximal emission at 465 nm. The reagents ...


    By Pickering Laboratories, Inc.

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