A successful anaerobic bioaugmentation was carried out on a trichloroethene (TCE)-contaminated aquifer at Dover Air Force Base, DE, using a microbial enrichment culture capable of dechlorinating TCE to ethene. A hydraulically controlled pilot system 12 18 m was constructed 15 m below ground surface in an alluvial aquifer to introduce nutrients and substrate into the groundwater. ...
ABSTRACT: This paper presents a comparative study of aerobic and anaerobic bioremediation of 1,2-dichloroethene (DCE) and vinyl chloride (VC), and includes laboratory bench scale testing of anaerobic biodegradation and a side by side field pilot test comparing the effectiveness of aerobic and anaerobic biodegradation processes. Manufacturing activities at an industrial facility in Ohio resulted ...
Untitled Document ABSTRACT: A pilot-study for treatment of trichloroethene (TCE) and dechlorination products, dichloroethene (DCE) and vinyl chloride (VC), is ...
PROBLEM STATEMENTThe Department of Defense (DoD) has identified hundreds of sites where groundwater is contaminated with chlorinated solvents; these represent one of the DoD’s largest remediation liabilities. In addition to their use in many industrial processes, chlorinated solvents have historically been used for cleaning and degreasing such diverse products as aircraft engines, automobile ...
Introduction Enhanced bioremediation is a process in which indigenous or inoculated micro-organisms (e.g., fungi, bacteria, and other microbes) degrade (metabolize) organic contaminants found in soil and/or ground water, converting them to innocuous end products. Nutrients, oxygen, or other amendments may be used to enhance bioremediation and contaminant desorption from subsurface materials. ...
BCI’s newest culture is a microbial mixture (consortia) which can transform chloroform (CF) to dichloroemthane (DCM), a transient intermediate which is then converted to a non-chlorinated product. We have demonstrated that the culture can dechlorinate at least 10 ppm of chloroform. Dechlorinating Bacteria in BCI Cultures. To address single compounds or a mixture of chlorinated ethenes, BCI ...
ABSTRACT This paper presents a comparative study of aerobic and anaerobic bioremediation of cis-1,2-dichloroethene (DCE) and vinyl chloride (VC). The study includes laboratory bench scale testing of anaerobic biodegradation followed by a side by side field pilot test comparing the effectiveness of aerobic and anaerobic biodegradation processes. Prior to initiating the pilot test, DCE and VC ...
Abstract Recent laboratory and field studies have shown that injection of emulsified edible oils can provide an effective, low-cost alternative for stimulating anaerobic biodegradation processes. A pilot-scale permeable reactive bio-barrier (PRBB) was installed at a perchlorate and chlorinated solvent impacted site by injecting 380 L of commercially available emulsion (EOS® ) ...
Batch incubation and flow-through column experiments were conducted to evaluate the use of emulsified soybean oil for in situ treatment of acid mine drainage. Addition of soybean oil, soluble substrates, and a microbial inoculum to the batch incubations resulted in complete depletion of SO4, 50% reduction in Fe, and an increase in pH to >6. A one time injection of emulsified soybean oil, lactate, ...
The use of vegetable oil as an electron donor to enhance the reductive dechlorination of chlorinated solvents as an in situ remediation technology is gaining significant traction. Vegetable oil is a cost-effective slow-release electron donor with greater hydrogen-release efficiency than other electron-donor products. However, neat vegetable oil can inhibit distribution in aquifers due to the oil ...
Untitled Document A three-tiered remediation approach is being used for successful field treatment of a 1,1- dichloroethene (1,1-DCE) groundwater plume. The plume is characterized by ...
Chlorinated solvents are a common threat to drinking water sources throughout the United States. While handling and disposal practices today are more closely regulated and monitored, this has not always been the case. With dry cleaners using tetrachloroethene (PCE) and industrial manufacturing facilities commonly using a variety of solvents from trichloroethene (TCE) to methylene chloride, past ...
ABSTRACT Bioremediation is a managed or spontaneous process in which microbiological processes are used to degrade or transform contaminants to less toxic or nontoxic forms, thereby mitigating or eliminating environmental contamination. In the presence of sufficient oxygen (aerobic conditions) and nutrients, microorganisms will ultimately convert many organic contaminants to carbon dioxide, ...
Introduction Prior operations at a former plating and hard anodizing facility in Santa Clara, California resulted in tetrachloroethene (PCE), trichloroethene (TCE), and cis-1,2-dichloroethene (DCE) impacts to soil and groundwater. In 1987, a groundwater monitoring program was implemented to monitor the plume. In 1998, CDM performed a natural attenuation evaluation for ...
Untitled Document Bioremediation, using CL-out microbes, clears the obstacles to Brownfield redevelopment by reducing wells contaminant concentrations in groundwater and removing the ...
What is bioremediation? Bioremediation is a treatment process that uses naturally occurring microorganisms (yeast, fungi, or bacteria) to break down, or degrade, hazardous substances into less toxic or nontoxic substances. Microorganisms, just like humans, eat and digest organic substances for nutrients and energy. In chemical terms, 'organic' compounds are those that contain carbon and hydrogen ...
Bioremediation is the method of choice to effectively clean up a small spill of hydrocarbons. In this case, five-hundred liters of BTEX has spilled from an above ground storage tank into a pasture that is located at the west edge of the Royal Roads University (RRU) grounds. The soil is classified as a Gleyed Eutric Brunisol based on recent laboratory analysis; it contains clay, but is fairly ...
The production of low-pH effluent with sulfate and metals is one of the biggest environmental concerns in the mining industry. The biological process for sulfate reduction has the potential to become a low-cost solution that enables the recovery of interesting compounds. The present study analyzed such a process in a horizontal-flow anaerobic immobilized biomass (HAIB) reactor, employing ...
ABSTRACT: To treat groundwater contaminants in situ, enhanced anaerobic bioremediation processes can be stimulated through addition of soluble substrates. At a dry cleaners site located in San Jose, California, the goal was to find a substrate that is long lasting and easily distributed into the saturated soils. After evaluating several alternatives, in situ bioremediation using an emulsified ...
EOS, or emulsified oil substrate, was used to stimulate anaerobic biodegradation of trichloroethene (TCE) and tetrachloroethene (PCE) at a former Army-owned manufacturing facility located in the Piedmont area of North Carolina. Previous use of chlorinated solvents at the facility resulted in soil and groundwater impacts. Ten years of active remediation utilizing soil vacuum extraction and air ...