assay Articles
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What Do You Know About TEER?
How often do you or your colleagues perform TEER (Transepithelial-Transendothelial Electrical Resistance) assays in a given week, and how confident are you in your results? The accuracy of your TEER assay measurements depends on four things: Temperature - It’s recommended you perform yours at exactly 37°C. Electrode positioning - The lower chamber electrode must touch the ...
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Intro for TEER Assay Article From Cellqart
How often do you or your colleagues perform TEER (Transepithelial-Transendothelial Electrical Resistance) assays in a given week, and how confident are you in your results? The accuracy of your TEER assay measurements depends on four things: Temperature - It’s recommended you perform yours at exactly 37°C. Electrode positioning - The lower chamber electrode must touch the ...
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Boost Assay Performance
Improve SARS-CoV-2 RT-PCR Assays Improve Automated Workflows and Boost Assay Performance Employing a process optimization approach to assay development involves breaking down the assay workflow into smaller modules, such as a mixing step or a reagent addition step, and then measuring and optimizing each module individually using MVS technology. Once the assay is optimized to its fullest extent, ...
By Artel, Inc.
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How Takeda streamlines assay transfer and troubleshooting with the MVS - Case Study
When one of Takeda’s quality control facilities wanted to improve assay success rates by reducing liquid handling variability, they turned to Artel. See how the MVS helps their assay process. Dozens of assays At any given time, Takeda’s quality control facility runs as many as a dozen different assays. Keeping them all performing within specifications can be time-consuming. ...
By Artel, Inc.
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Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum
This research was undertaken to develop tools that facilitate the industrial application of an immobilized loofah–fungi system to degrade Direct Black 22 (DB22) azo dye. In laboratory-scale tests, the DB22, and loofah as support, were used. Assays without loofah were used as a free-cells control. The use of natural carriers to facilitate adhesion and growth of the fungi has shown favorable ...
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Dual luciferase assay system for rapid assessment of gene expression in saccharomyces cerevisiae
A new reporter system has been developed for quantifying gene expression in the yeast Saccharomyces cerevisiae. The system relies on two different reporter genes, Renilla and firefly luciferase, to evaluate regulated gene expression. The gene encoding Renilla luciferase is fused to a constitutive promoter (PGK1 or SPT15) and integrated into the yeast genome at the CAN1 locus as a control for ...
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Genotoxicity evaluation of atmospheric environments of subway stations in Seoul using Tradescantia micronucleus assay
Airborne pollutants in subway facilities can be harmful to passengers. This study was done to examine whether the suspended particulates have clastogenic effects on the biological systems. Total suspended particulates were collected from subway stations in Seoul. Aqueous extracts from the particulates were tested with the Tradescantia micronucleus assay. All the plant cuttings exposed to the ...
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Effects of proton beams from the folded tandem ion accelerator on DNA damage in mouse leukocytes using the comet assay
Single cell gel electrophoresis or the comet assay is a sensitive technique to quantify different types of DNA damage, e.g. single strand breaks, double strand breaks, base damage at single cell level of any dividing or non-dividing eukaryotic cell. This paper describes the effect of 3.3 MeV proton beams from the folded tandem ion accelerator on DNA damage in mouse leukocytes using the comet ...
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Rapid molecular identification of fecal origin-colonies growing on Enterococcus spp.-specific culture methods
The mEI, Chromocult® enterococci, and m-Enterococcus culture-based methods used to assess water quality by the detection of Enterococcus spp. were first compared in terms of sensitivity using (1) 41 different type strains of Enterococcus spp. and (2) environmental colonies identified by 16S rRNA sequencing. Then, two specific-rtPCR assays targeting Enterococcus spp. and Enterococcus ...
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Bulked segregant analysis using the goldengate assay to locate the Rpp3 Locus that confers resistance to soybean rust in soybean
Few resistance loci to soybean rust (SBR), caused by Phakopsora pachyrhizi Syd., have been genetically mapped and linked to molecular markers that can be used for marker assisted selection. New technologies are available for single nucleotide polymorphism (SNP) genotyping that can be used to rapidly map traits controlled by single loci such as resistance to SBR. Our objective was to demonstrate ...
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Indirect Determination of Gold Purity by Measurement of Impurities with Flame Atomic Absorption Spectroscopy
The determination of the purity of scrap and refined gold is a very important procedure for the financial markets, the jewelry business and the electronics industry. Over the years, the classical fire assay technique has been used for gold determination but this is a very labor-intensive procedure, and prone to many operator errors. Hundreds of books have been written with special steps ...
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Functional Assays for Hit Confirmation
Diagnosis of HIT, in patients with a significant HIT clinical probability (4Ts' score or HIT Expert Probability score), and positive for heparin dependent antibodies, must be confirmed with functional assays. These assays are performed at a low (0.10 to 1.00 lU/ml) and a high (10 to 100 lU/ml) heparin concentration, and possibly without heparin, as this helps identification of aspecific ...
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Quantitative detection of human enteric adenoviruses in river water by microfluidic digital polymerase chain reaction
We describe an assay for simple and accurate quantification of human enteric adenoviruses (EAdVs) in water samples using a recently developed quantification method named microfluidic digital polymerase chain reaction (dPCR). The assay is based on automatic distribution of reaction mixture into a large number of nanolitre-volume reaction chambers and absolute copy number quantification from the ...
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Algal-available phosphorus entering the gulf of finland as estimated by Algal assays and chemical analyses
Received for publication August 11, 2008. Algal-available phosphorus (Paa) in river water and wastewater entering the Gulf of Finland (a Baltic Sea sub-basin) was estimated by a fresh-water and a brackish-water modification of the dual-culture algal assay. The assay results were further related to those obtained by routine chemical analyses. According to the brackish-water assay, an average of ...
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Acute Toxicity of Nine Pesticides as Determined with Conventional Assays and Alternative Microbiotests
Abstract : Conventional bioassays in aquatic toxicology are time and space consuming and depend on the continuous culture and maintenance of the test species. Since Toxkit microbiotests do not suffer from these handicaps, they seem to be a tempting alternative. In order to find out the potential and the limitation of these new microbiotests the sensitivity of 4 Toxkit microbiotests has been ...
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Evaluation of quantitative polymerase chain reaction assays targeting
Mycobacterium avium ,M. intracellulare , andM. avium subspeciesparatuberculosis in drinking water biofilmsMycobacterium avium (MA), Mycobacterium intracellulare (MI), and Mycobacterium avium subsp. paratuberculosis (MAP) are difficult to culture due to their slow growing nature. A quantitative polymerase chain reaction (qPCR) method for the rapid detection of MA, MI, and MAP can be used to provide data supporting drinking water biofilms as potential sources of human exposure. The aim of this study ...
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Vanadium bioavailability and toxicity to soil microorganisms and plants
Vanadium, V, is a redox‐sensitive metal that in solution, under aerobic conditions, prevails as the oxyanion vanadate(V). There is little known regarding vanadium toxicity to soil biota and the present study was set up to determine the toxicity of added vanadate to soil organisms and to investigate the relationship between toxicity and vanadium sorption in soils. Five soils with contrasting ...
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Development of an enzyme‐linked immunosorbent assay and a beta‐1 adrenergic receptor–based assay for monitoring the drug atenolol
Two approaches for monitoring atenolol (ATL) were applied: an immunochemical assay and a competitive‐binding assay, based on the interaction between ATL and its target receptor, β1 adrenergic receptor (β1AR). Polyclonal antibodies (Abs) for ATL were generated, and a highly specific microplate immunochemical assay, i.e., an enzyme‐linked immunosorbent assay (ELISA), for its detection was ...
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Alginate biopolymer production by Azotobacter chroococcum from whey degradation
Abstract: The potential of three Azotobacter chroococcum strains for whey degradation and alginate production were investigated. After dilution, samples were spread plated on isolation agar and Manitol agar and incubated at 30 °C for 24 h. Microorganisms were screened for their ability to whey degradation and alginate production based on colony morphology, negative and capsule staining, ability ...
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Development and assessment of a novel Arxula adeninivorans androgen screen (A-YAS) assay and its application in analysis of cattle urine
Abstract The novel A-YAS assay for the detection of androgenic activity in liquid samples such as urine has been developed and assessed. The assay is based on transgenic Arxula adeninivorans yeast cells as the bio-component. The cells were engineered to co-express the human androgen receptor (hAR) gene and the inducible phytase reporter gene (phyK, derived from Klebsiella sp. ASR1), under the ...
By QuoData GmbH
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