assay Articles
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How Takeda streamlines assay transfer and troubleshooting with the MVS - Case Study
When one of Takeda’s quality control facilities wanted to improve assay success rates by reducing liquid handling variability, they turned to Artel. See how the MVS helps their assay process. Dozens of assays At any given time, Takeda’s quality control facility runs as many as a dozen different assays. Keeping them all performing within specifications can be time-consuming. ...
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What Do You Know About TEER?
How often do you or your colleagues perform TEER (Transepithelial-Transendothelial Electrical Resistance) assays in a given week, and how confident are you in your results? The accuracy of your TEER assay measurements depends on four things: Temperature - It’s recommended you perform yours at exactly 37°C. Electrode positioning - The lower chamber electrode must touch the ...
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Intro for TEER Assay Article From Cellqart
How often do you or your colleagues perform TEER (Transepithelial-Transendothelial Electrical Resistance) assays in a given week, and how confident are you in your results? The accuracy of your TEER assay measurements depends on four things: Temperature - It’s recommended you perform yours at exactly 37°C. Electrode positioning - The lower chamber electrode must touch the ...
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Boost Assay Performance
Improve SARS-CoV-2 RT-PCR Assays Improve Automated Workflows and Boost Assay Performance Employing a process optimization approach to assay development involves breaking down the assay workflow into smaller modules, such as a mixing step or a reagent addition step, and then measuring and optimizing each module individually using MVS technology. Once the assay is optimized to its fullest extent, ...
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Scaling up and kinetic model validation of Direct Black 22 degradation by immobilized Penicillium chrysogenum
This research was undertaken to develop tools that facilitate the industrial application of an immobilized loofah–fungi system to degrade Direct Black 22 (DB22) azo dye. In laboratory-scale tests, the DB22, and loofah as support, were used. Assays without loofah were used as a free-cells control. The use of natural carriers to facilitate adhesion and growth of the fungi has shown favorable ...
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Dual luciferase assay system for rapid assessment of gene expression in saccharomyces cerevisiae
A new reporter system has been developed for quantifying gene expression in the yeast Saccharomyces cerevisiae. The system relies on two different reporter genes, Renilla and firefly luciferase, to evaluate regulated gene expression. The gene encoding Renilla luciferase is fused to a constitutive promoter (PGK1 or SPT15) and integrated into the yeast genome at the CAN1 locus as a control for ...
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Comparison of the Conventional Algal Assay and the Algaltoxkit FTM Microbiotest for Toxicity Evaluation of Sediment Pore Waters
Abstract : Conventional algal tests have not been utilized to their full potential for routine toxicity assessments. This is mainly due to the complexicity of these assays and the fact that many operators are still encountering practical difficulties. In view of the need for a cost-effective alternative to standard algal assays for routine testing, a new culture/maintanance free algal test was ...
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Indirect Determination of Gold Purity by Measurement of Impurities with Flame Atomic Absorption Spectroscopy
The determination of the purity of scrap and refined gold is a very important procedure for the financial markets, the jewelry business and the electronics industry. Over the years, the classical fire assay technique has been used for gold determination but this is a very labor-intensive procedure, and prone to many operator errors. Hundreds of books have been written with special steps ...
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Evaluation of quantitative polymerase chain reaction assays targeting
Mycobacterium avium ,M. intracellulare , andM. avium subspeciesparatuberculosis in drinking water biofilmsMycobacterium avium (MA), Mycobacterium intracellulare (MI), and Mycobacterium avium subsp. paratuberculosis (MAP) are difficult to culture due to their slow growing nature. A quantitative polymerase chain reaction (qPCR) method for the rapid detection of MA, MI, and MAP can be used to provide data supporting drinking water biofilms as potential sources of human exposure. The aim of this study ...
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Functional Assays for Hit Confirmation
Diagnosis of HIT, in patients with a significant HIT clinical probability (4Ts' score or HIT Expert Probability score), and positive for heparin dependent antibodies, must be confirmed with functional assays. These assays are performed at a low (0.10 to 1.00 lU/ml) and a high (10 to 100 lU/ml) heparin concentration, and possibly without heparin, as this helps identification of aspecific ...
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Assessing Sediment Contamination using Six Toxicity Assays
Abstract: An evaluation of sediment toxicity at Lake Orta, Italy was conducted to compare a toxicity test battery of 6 assays and to evaluate the extent of sediment contamination at various sediment detphs. Lake Orta received excessive loadings of copper and ammonia during the 1900\'s until a large remediation effort was conducted in 1989-90 using lime addition. Since that time, the lake has ...
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Assessing the genotoxicity of imidacloprid and RH-5849 in human peripheral blood lymphocytes in vitro with comet assay and cytogenetic tests
Untitled Document A combined approach employing comet assay and micronucleus (MN) and sister chromatid exchanges (SCE) tests was utilized to assess the genotoxicity of two pesticides, imidacloprid [1-(6-chloro-3-pyridylmethyl)-N-nitro-imidazolidin-2-ylideneamine] and RH- 5849 [20-benzoyl-10-tert-butylbenzoylhydrazine], on human peripheral blood lymphocytes in vitro. No ...
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Rapid molecular identification of fecal origin-colonies growing on Enterococcus spp.-specific culture methods
The mEI, Chromocult® enterococci, and m-Enterococcus culture-based methods used to assess water quality by the detection of Enterococcus spp. were first compared in terms of sensitivity using (1) 41 different type strains of Enterococcus spp. and (2) environmental colonies identified by 16S rRNA sequencing. Then, two specific-rtPCR assays targeting Enterococcus spp. and Enterococcus ...
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Genotoxicity evaluation of atmospheric environments of subway stations in Seoul using Tradescantia micronucleus assay
Airborne pollutants in subway facilities can be harmful to passengers. This study was done to examine whether the suspended particulates have clastogenic effects on the biological systems. Total suspended particulates were collected from subway stations in Seoul. Aqueous extracts from the particulates were tested with the Tradescantia micronucleus assay. All the plant cuttings exposed to the ...
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Quantitative detection of human enteric adenoviruses in river water by microfluidic digital polymerase chain reaction
We describe an assay for simple and accurate quantification of human enteric adenoviruses (EAdVs) in water samples using a recently developed quantification method named microfluidic digital polymerase chain reaction (dPCR). The assay is based on automatic distribution of reaction mixture into a large number of nanolitre-volume reaction chambers and absolute copy number quantification from the ...
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Enzyme-linked immunosorbent assay for triclocarban in aquatic environments
A sensitive, competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of triclocarban (TCC) in waters and sediments. Haptens were synthesized by derivatizing the paraposition of a phenyl moiety of TCC. The synthesized hapten was then coupled to bovine thyroglobulin to be used as an immunogen, based on which, a high affinity monoclonal antibody 4D5 was ...
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Vanadium bioavailability and toxicity to soil microorganisms and plants
Vanadium, V, is a redox‐sensitive metal that in solution, under aerobic conditions, prevails as the oxyanion vanadate(V). There is little known regarding vanadium toxicity to soil biota and the present study was set up to determine the toxicity of added vanadate to soil organisms and to investigate the relationship between toxicity and vanadium sorption in soils. Five soils with contrasting ...
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Algal-available phosphorus entering the gulf of finland as estimated by Algal assays and chemical analyses
Received for publication August 11, 2008. Algal-available phosphorus (Paa) in river water and wastewater entering the Gulf of Finland (a Baltic Sea sub-basin) was estimated by a fresh-water and a brackish-water modification of the dual-culture algal assay. The assay results were further related to those obtained by routine chemical analyses. According to the brackish-water assay, an average of ...
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Rapid Positive Release Of Commercially Sterile Products
Manufacturers of dairy and plant-based Ultra High Temperature (UHT) and Extended Shelf Life (ESL) products, can now release finished product faster with a more streamlined workflow using the Oculer Commercial Sterility Check (CS-Check) assay. The reduced time to result leads to lower warehouse costs, plus early warning of possible spoilage problems thereby reducing waste production and enabling ...
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Bulked segregant analysis using the goldengate assay to locate the Rpp3 Locus that confers resistance to soybean rust in soybean
Few resistance loci to soybean rust (SBR), caused by Phakopsora pachyrhizi Syd., have been genetically mapped and linked to molecular markers that can be used for marker assisted selection. New technologies are available for single nucleotide polymorphism (SNP) genotyping that can be used to rapidly map traits controlled by single loci such as resistance to SBR. Our objective was to demonstrate ...
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