bacterial DNA Articles
-
Monitoring of three drinking water treatment plants using flow cytometry
A 4-month sampling campaign has been conducted for the monitoring of three drinking water treatment plants using flow cytometry and culture-based methods to provide information related to changes in bacterial concentration according to treatments. Flow cytometry is a fast and user-friendly technique enabling bacteria quantification and viability assessment in less than 1 hour. Specific profiles ...
-
Bacterial diversity in antibiotic wastewater treatment
The bacterial diversity of an antibiotic industrial wastewater treatment system was analyzed to provide the information required for further optimization of this process and for identification of bacterial strains that perform improved degradation of antibiotic industrial wastewater. The total bacterial DNA of samples collected at three stages (aeration, precipitation, and idle) during the ...
-
A pcr-based method for monitoring legionella pneumophila in water samples detects viable but noncultivable legionellae that can recover their cultivability
Legionella pneumophila is the causative agent of Legionnaires' disease. This bacterium is ubiquitous in aqueous environments and uses amoebae as an intracellular replicative niche. Real-time PCR has been developed for rapid detection of Legionella DNA in water samples. In addition to culturable bacteria, this method may also detect dead and viable but noncultivable (VBNC) legionellae. In order to ...
-
Third Natural Eukaryotic Epigenetic Mark Found
DNA carries the blueprint to build the body, but it is a living document: the design can be adjusted by epigenetic markers. In humans and other eukaryotes, two major epigenetic marks are known. A group at the Marine Biology Laboratory (MBL) now found a third epigenetic mark in this freshwater invertebrate, Adineta vaga, which has previously been found only in bacteria. For the first time, a ...
-
Effect of the disinfection agents chlorine, UV irradiation, silver ions, and TiO2 nanoparticles/near-UV on DNA molecules
Extracellular DNA in municipal wastewater and effluents from hospitals and R&D laboratories contains antimicrobial resistance and recombinant genes that are today considered as a new class of emerging contaminants. The objective of this research was to investigate the effect of disinfection agents on the integrity of DNA molecules by using real-time PCR. Escherichia coli cell suspensions and ...
-
SARS-CoV-2 (Coronavirus) Detection Workflow for Research Use Only
Overview of the SARS-CoV-2 (Coronavirus) Detection Process In December 2019 the novel SARS-CoV-2 (formerly named 2019-nCoV) was identified in Wuhan, the capital of China’s province Hubei. Based on its rapid spreading with more than 105,000,000 confirmed cases by beginning of February 2021 the World Health Organization declared the outbreak a public health emergency of international ...
-
CRISPR is coming to agriculture — with big implications for food, farmers, consumers and nature
Gene editing offers dramatic advances in speed, scope and scale of genetic improvement. It also offers an opportunity for more nuanced GMO governance. Very few technologies truly merit the epithet “game changer” — but a new genetic engineering tool known as CRISPR-Cas9 is one of them. Since we first developed the ability to alter the genetic material inside a plant or animal in ...
By Ensia
-
SARS-CoV-2 (Coronavirus) Detection in Wastewater
SARS-CoV-2 detection workflow in wastewater Monitoring wastewater for SARS-CoV-2 – or Coronavirus as it is more commonly known – can be a valuable data resource for an effective COVID-19 management. However, there are several challenges involved in detecting biological parameters in wastewater. Fortunately, there are also several solutions available to overcome them. Wastewater ...
-
Biological Water Testing
Improving Water Monitoring for Pathogens and other Microorganisms Water quality and purity is essential for public and environmental health but monitoring drinking water and wastewater for microbial orgamisms can be difficult and time consuming. In this article we want to highlight the Real-time PCR-based detection methods for some major pathogens and microorganisms that increases analysis ...
-
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16s rrna.
We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S rRNA, all the same length, by denaturing gradient gel electrophoresis (DGGE). DGGE analysis of different microbial communities demonstrated the presence of up to 10 ...
-
Analysis of the bacterial community changes in soil for septic tank effluent treatment in response to bio-clogging
Soil columns were set up to survey the bacterial community in the soil for septic tank effluent treatment. When bio-clogging occurred in the soil columns, the effluent from the columns was in poorer quality. To evaluate changes of the soil bacterial community in response to bio-clogging, the bacterial community was characterized by DNA gene sequences from soil samples after polymerase chain ...
-
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA
We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S rRNA, all the same length, by denaturing gradient gel electrophoresis (DGGE). DGGE analysis of different microbial communities demonstrated the presence of up to 10 ...
-
Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium escherichia coli
We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S rrna, all the same length, by denaturing gradient gel electrophoresis (DGGE). DGGE analysis of different microbial communities demonstrated the presence of up to 10 ...
-
High frequency of
Helicobacter pylori DNA in drinking water in Kermanshah, Iran, during June–November 2012To gain a better understanding of transmission and selecting appropriate measures for preventing the spread of Helicobacter pylori, the aim of this study was to investigate the prevalence of H. pylori in drinking water samples in Kermanshah, Iran. Drinking water samples were collected from around Kermanshah and filtered through 0.45 μm nitrocellulose filters. The bacterial sediment was ...
-
Overview of Recombinant DNA Technology
Introduction Screening microorganisms for valuable products is the key to industrial production. Initially, the focus was on finding microbes from different natural sources. However, there are limitations to the best way to produce the desired product. Although the strain modification method is very successful, the process of strain production is tedious, time-consuming, expensive, and random. At ...
By BOC Sciences
-
Start with the ecosystem, not nutrients, to stop algal blooms, scientists say
Human activity is warming the climate around the world and polluting the environment Microcystin, the substance that makes algal blooms toxic Krausfeldt’s study unveiled 30 kinds of cyanobacteria that had never before been detected Summer brings hours of sunshine, lazing about with friends and enjoying the warmth of the season, but it also wakes up a sinister plague that ...
By LG Sonic
-
Application of Flow Cytometry for Microbiological Monitoring of Pharmaceutical Grade Water
Online Water Bioburden Analysis As the main excipient for the pharmaceutical industry, water is integral to patient safety and quality of pharmaceutical products (ISPE 2011). Colony Forming Units (CFU) are defined in the pharmacopoeias as the unit for assessing microbiological quality. CFUs are quantified using the traditional Heterotrophic Plate Count (HPC) method, a three to five-day process ...
Need help finding the right suppliers? Try XPRT Sourcing. Let the XPRTs do the work for you