Coliform bacteria detection Articles

Development and validation of a FISH-based method for the detection and quantification of <italic>E. coli</italic> and coliform bacteria in water samples

Development and validation of a FISH-based method for the detection and quantification of E. coli and coliform bacteria in water samples

Monitoring of microbiological contaminants in water supplies requires fast and sensitive methods for the specific detection of indicator organisms or pathogens. We developed a protocol for the simultaneous detection of E. coli and coliform bacteria based on the Fluorescence in situ Hybridization (FISH) technology. This protocol consists of two approaches. The first allows the ...

Jan. 1, 2011

By IWA Publishing

Multiplex PCR for detection of water-borne bacteria

Microbial water-borne diseases still affect developing countries and are major water quality concerns throughout the world. Routine culture-based methods of identifying bacterial pathogens in water sources are laborious and time-consuming. Recently, the use of molecular techniques such as the polymerase chain reaction (PCR) has provided rapid and highly promising detection methods. In this ...

Jul. 28, 2016

By IWA Publishing

Bacteroides bacteria and their bacteriophages

There are many sources that can contribute to fecal contamination in water bodies such as domestic wastewaters, wastewater treatment plants, husbandry and farms. Coliform bacteria are a group of bacteria that inhabit in the digestive tracts of human and warm-blooded animals. It can be found in human and animal feces. Therefore, the presence of coliform bacteria can indicate fecal contamination, ...

Dec. 22, 2014

By IWA Publishing

Potentially pathogenic bacteria isolated from different tropical waters in Sri Lanka

This study investigated the bacteriological contamination of different water sources in Sri Lanka. Source waters (n = 74) including bottled water, well water and surface water were assessed for enumerating total coliforms and faecal coliforms using the membrane filtration method. The results showed that 18.5 and 14.8% of bottled water samples were contaminated with total ...

Nov. 20, 2013

By IWA Publishing

Chlorine disinfection of <italic>Pseudomonas aeruginosa</italic>, total coliforms, <italic>Escherichia coli</italic> and <italic>Enterococcus faecalis</italic>: revisiting reclaimed water regulations

Chlorine disinfection of Pseudomonas aeruginosa, total coliforms, Escherichia coli and Enterococcus faecalis: revisiting reclaimed water regulations

Pathogenic organisms can be transmitted orally through drinking water or through skin and mucosae by both direct and indirect contact, and their presence in water thus has a negative impact on public health. In wastewater treatment plants (WWTP), water is disinfected to inactivate pathogens. The quantification of several microbial indicators in aquatic systems is required to estimate the ...

Dec. 1, 2011

By IWA Publishing

Comparing wastewater chemicals, indicator bacteria concentrations, and bacterial pathogen genes as fecal pollution indicators

The objective of this study was to compare fecal indicator bacteria (FIB) (fecal coliforms, Escherichia coli [EC], and enterococci [ENT]) concentrations with a wide array of typical organic wastewater chemicals and selected bacterial genes as indicators of fecal pollution in water samples collected at or near 18 surface water drinking water intakes. Genes tested included esp (indicating ...

Jan. 13, 2009

By Soil Science Society of America (SSSA)

Elimination of enteric indicators and pathogenic bacteria in secondary effluents and lake water by solar disinfection (SODIS)

The reduction of enteric indicators (fecal coliforms (FC) and Enterococcus faecalis) and elimination of pathogenic bacteria (Salmonella spp. and Staphylococcus aureus) in the secondary effluents and lake water by solar disinfection (SODIS) was studied in this article. FC, E. faecalis, Salmonella spp. and ...

Feb. 28, 2013

By IWA Publishing

Determination of several potential virulence factors in non-o1 <italic>Vibrio cholerae</italic>, <italic>Pseudomonas aeruginosa</italic>, faecal coliforms and streptococci isolated from Marrakesh groundwater

Determination of several potential virulence factors in non-o1 Vibrio cholerae, Pseudomonas aeruginosa, faecal coliforms and streptococci isolated from Marrakesh groundwater

The dynamic, hemolytic and hemagglutination activities and the antibiotic resistance of non-O1 Vibrio cholerae, Pseudomonas aeruginosa, faecal coliforms (FC) and faecal streptococci (FS), isolated by standard membrane filtration methods from suburban and rural groundwater supplies, were carried out. Detectable non-O1 V. cholerae and P. aeruginosa ...

Apr. 1, 2010

By IWA Publishing

Rapid ultrafiltration concentration and biosensor detection of enterococci from large volumes of florida recreational water

Monitoring recreational waters for fecal contamination by standard methodologies involves culturing indicator bacteria, such as fecal coliforms and enterococci. Delayed reporting of microbial water quality parameters increases the likelihood of public exposure to pathogens of fecal origin, making the development of rapid methods important for ...

Aug. 1, 2008

By American Society for Microbiology

Methodology for Forensic Investigation of Potable Water Quality in Municipal Distribution Systems

Abstract During routine regulatory potable water quality sampling in distribution systems related to the Total Coliform Rule, utilities can sometimes experience anomalous detections of total coliform bacteria and/or fecal coliform bacteria in the distribution system samples. Frequently, the regulatory-required retesting of the same sample locations reveals no detection of bacteria, and thus ...

Oct. 13, 2007

By Water Environment Federation (WEF)

Microbiological pollution of surface water in the upper–middle basin of the Reconquista river (Argentina): 2010–2011 monitoring

A microbiological monitoring was conducted on the water at six locations of the Reconquista river, a polluted peri–urban water body of Argentina. Samples were taken from surface water of three tributary streams in the dam outlet and 18 km downstream; samples from the sediments of one of the streams were also taken. For all samples the presence and quantity of coliform bacteria (total ...

May 22, 2013

By Inderscience Publishers

Estimated occupational risk from Bioaerosols generated during land application of class B biosolids

Some speculate that bioaerosols from land application of biosolids pose occupational risks, but few studies have assessed aerosolization of microorganisms from biosolids or estimated occupational risks of infection. This study investigated levels of microorganisms in air immediately downwind of land application operations and estimated occupational risks from aerosolized microorganisms. In all, ...

Oct. 23, 2008

By Soil Science Society of America (SSSA)

Occurrence and antibiotic resistance of escherichia coli O157:H7 in a watershed in north-central Indiana

Received for publication February 11, 2008. The Wildcat Creek in north-central Indiana is an impaired stream with historically high fecal coliform counts. This study evaluated the presence of both fecal coliforms and Escherichia coli O157:H7 at five sites in rural and urban areas in the eastern part of the Wildcat Creek watershed. Escherichia coli O157:H7 was isolated by immunomagnetic ...

Mar. 25, 2009

By Soil Science Society of America (SSSA)

Occurrence of norovirus and other enteric viruses in untreated groundwaters of Korea

A total of 39 water samples from 23 different groundwater wells in Korea were collected and analyzed in order to monitor the occurrence of norovirus (NoV) and other indicator microbes as the first part of a national survey of groundwater. More than 500 L of untreated groundwater were filtered through 1MDS filters. Following elution and concentration by organic flocculation, PCR and sequence ...

Sep. 1, 2011

By IWA Publishing

Microbiological quality of ice and ice machines used in food establishments

The ice used in the food industry has to be safe and the water used in ice production should have the quality of drinking water. The consumption of contaminated ice directly or indirectly may be a vehicle for transmission of pathogenic bacteria to humans producing outbreaks of gastrointestinal diseases. The objective of this study was to monitor the microbiological quality of ice, the water ...

Mar. 25, 2017

By IWA Publishing

Hand-pumps as reservoirs for microbial contamination of well water

The retention and release of total coliforms and Escherichia coli was investigated in hand-pumps removed from tubewells tapping a faecally contaminated aquifer in Matlab, Bangladesh, and from a new hand-pump deliberately spiked with E. coli. All hand-pumps were connected to reservoirs of sterile water and flushed. Faecal coliforms were observed in the discharge from all three of ...

Dec. 1, 2011

By IWA Publishing

Maintenance strategies for on-site water disinfection by ultraviolet lamps on dairy farms

Bacteria have been detected in many water supplies on dairy farms, and the water used to wash milking equipment should be free of bacteria. This study evaluated two ultraviolet (UV) technologies, with and without automatic sleeve cleaning device, as on-farm water disinfection system. No fouling was observed during 6 months of continuous operation of the self-cleaning UV system. The dose provided ...

Feb. 1, 2011

By IWA Publishing

Evaluation of multiple-locus variable-number tandem repeat analysis (MLVA) for genotyping of Escherichia coli isolated from Karaj River

Most microbiological water quality regulations rely upon the detection of indicators of fecal pollution, such as coliform bacteria, or more specifically Escherichia coli. In order to further understand the source, fate, and implications for water quality regulation, environmental E. coli isolates should be assessed genetically to observe various levels of ...

Jun. 5, 2017

By IWA Publishing

The DNA Revolution for Water Professionals

Imagine trying to solve a problem when you are provided just 1% of the total data. This is essentially the case in analyzing water and wastewater microbiomes. Conventional microbiological testing methods such as culture and microscopy can identify only about 1% of microorganisms that are contained in a ...

Jul. 14, 2016

By LuminUltra Technologies Ltd.

3 Common Water Quality Concerns

About 10 percent of pond owners experience water quality problems in their ponds. Unfortunately, most pond owners pay little attention to managing the quality of water and never get it tested. As a result, the problem is detected only when it gets worst. Water quality is influenced by both natural processes and external environmental factors.Natural factors may include the types of rock and soil ...

Jun. 8, 2015

By LG Sonic

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