Aquavalens: Use of new tools for improved treatment methods for the detection of pathogens in drinking water - Case Study
Case: use of new tools for improved treatment methods for the detection of pathogens in drinking water The aim of Cluster 3 of the Aquavalens project is to use the newly developed analytical tools, technologies or platforms for the detection of microbial pathogens and indicators in practical settings. Within WP 10, these tests are performed in large drinking water systems located in the United ...
Evaluation of a BWTK for detection of total coliforms,
E. coliand emerging pathogens from drinking water: comparison with standard MPN method
A defined substrate method media in bacteriological water testing kit (BWTK) has been developed to recover and detect the presence/absence of total coliforms and emerging pathogens from drinking water without the need for the confirmatory or complete tests. This method is based on technology that uses a hydrolysable substrate as a specific indicator- nutrient for the target microbes. To perform ...
Access to household water connections remains low in sub-Saharan Africa, representing a public health concern. Previous studies have shown water stored in the home to be more contaminated than water at the source; however, the mechanisms of post-supply contamination remain unclear. Using water quality measurements and structured observations of households in Bagamoyo, Tanzania, this study ...
Detection of viable bacterial pathogens in a drinking water source using propidium monoazide-quantitative PCR
A cell viability assay was applied to measure bacterial enteric pathogens in a river in southern Ontario, Canada that is used as a source of drinking water. Pathogen concentrations were measured using both propidium monoazide (PMA)-quantitative polymerase chain reaction (PCR) and quantitative PCR (qPCR) without PMA pretreatment to compare viable and total (live and dead) cells. The pathogens ...
Rapid, specific and quantitative polymerase chain reaction (PCR) detection of pathogenic protozoa
Entamoeba histolyticafor drinking water supply
In this study, a quantitative species-specific polymerase chain reaction (PCR) method to rapidly detect E. histolytica in water is developed. First, the specificity of E. histolytica PCR detection was verified by using species-specific primers of 16S-like rRNA genes to clearly differentiate it from the closely related amoebae species E. dispar and E. moshkovskii. The sensitivity of this method ...
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