genotyping Articles
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Graphic analysis of genotype by environment interaction for lentil yield in iran
Selection of lentil (Lens culinaris Medik) cultivars with wide adaptability across diverse farming environments is important, before recommending them to achieve a high rate of cultivar adoption. Seed yield of 11 lentil genotypes, tested in a randomized complete-block design with four replicates across 20 environments in Iran, was analyzed using site regression (SREG) stability model. The biplot ...
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Biplot analysis of genotype X Environment interaction: Proceed with caution
Biplot analysis has been used for studying genotype x environment interaction (GE) or any two-way table. Its descriptive and visualization capabilities along with the availability of user-friendly software have enabled plant scientists to examine any two-way data by a click on a computer button. Despite widespread use, the validity and limitations of biplot analysis have not been completely ...
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Effects of toxic Microcystis genotypes on natural colony formation and mechanism involved
The cyanobacterium Microcystis occurs as colonies of different sizes with varying abundance of toxic genotypes versus non-toxic genotypes under natural conditions. To investigate the effects of toxic Microcystis genotypes on natural colony formation, samples collected from the mainstream of Haihe River from July to October 2015 were sieved into four colony classes with sizes of <8 μm, 8–20 ...
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Potassium induced salinity tolerance in wheat (Triticum aestivum L.)
Water culture experiments were conducted to study the response of ten wheat genotypes to external K application (10 mmol KCI dm_3) at seedling stage under saline condition (0 and 100 mmol NaCl dm_3). The data showed that there was an increase in the shoot and root length with the application of external K. The increase was more pronounced under control than under saline conditions. The better ...
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Genetic diversity for pink snow mold resistance in greens-type annual bluegrass
Unseeded annual bluegrass (Poa annua L.) is an important component of golf greens in many regions of Canada and the United States. Although this turfgrass species has desirable playing attributes, it suffers from susceptibility to environmental and biological stresses including subfreezing temperatures and snow molds. In this study, we compared 29 genotypes collected from golf greens located in ...
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Cloning and Characterization of Microsatellite Loci in a Gorgonian Coral, Junceella juncea (Anthozoa, Octocorallia, Ellisellidae) and Its Application in Clonal Genotyping
We attempted to isolate microsatellites from a Symbiodinium-free gorgonian octocoral, Junceella juncea, using two methods, partial genomic library screening and enrichment. Among the 3856 clones screened by the partial library method, 10 possibly positive signals were found, and 3 of them could be used to design primers and amplified consistently. In contrast, only one locus isolated by the ...
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Response of oat genotypes to fusarium head blight in eastern Canada
Recent investigations in northern Europe and western Canada suggested that mycotoxins caused by Fusarium head blight (FHB) could be a potential problem for oat (Avena sativa L.) production and oat food safety. Here we report studies conducted in eastern Canada to address this issue. In one study, oat genotypes of diverse origin were evaluated for grain and groat deoxynivalenol (DON) contamination ...
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Crop genotype and plant population density impact flowering Phenology and Synchrony between cropped and volunteer spring
Wheat (Triticum aestivum L.) is one of many crops into which novel traits have been incorporated using recombinant DNA technology, and thus may require segregation from nontransgenic wheat. Volunteer wheat populations, which cannot be selectively removed from wheat crops, pose a challenge to segregation because they may serve to facilitate trait movement. However, diverse flowering phenologies ...
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Global cytosine methylation in Daphnia magna depends on genotype, environment and their interaction
The authors characterized global cytosine methylation levels in two different genotypes of the ecotoxicological model organism Daphnia magna after exposure to a wide array of biotic and abiotic environmental stressors. The study aimed to improve the authors' understanding of the role of cytosine methylation in the organism‘s response to environmental conditions. The authors observed a ...
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Exogenous Transposable Elements Circumvent Identity-Based Silencing Permitting the Dissection of Expression Dependent Silencing
The propagation of epigenetic marks has received a great deal of attention, yet the initiation of epigenetic silencing of a new transgene, virus, or transposable element (TE) remains enigmatic. The overlapping and simultaneous function of multiple silencing mechanisms has obscured this area of investigation. Here, we have revealed two broad mechanisms that can initiate silencing independently: ...
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Morphology and Genetics of biomass in little bluestem
Little bluestem [Schizachyrium scoparium (Michx.) Nash] was the most important dominant species of uplands in the tallgrass prairie. Presently, it is a dominant species, most significantly on coarse-textured soils, in the mixed-grass prairie region of the United States and Canada. This wide geographical adaptation to dry soils suggests little bluestem has potential for biomass production in areas ...
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Malate exudation by six aerobic rice genotypes varying in zinc uptake efficiency
Received for publication February 2, 2009. Zinc (Zn) uptake by plant roots from soils low in plant-available Zn may be increased by Zn-mobilizing rhizosphere processes, including exudation of low-molecular-weight organic anions. A rhizotron experiment with a low Zn clay soil and a nutrient solution experiment were conducted to test if this occurs in six rice (Oryza sativa L.) genotypes varying in ...
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Thermotolerant
Acanthamoeba spp. isolated from therapeutic hot springs in northwestern IranThis study was conducted to address the distribution of Acanthamoeba genotypes in therapeutic hot springs in Iran. Sixty water and sediment samples were collected from bicarbonate, sulphur, and sodium chloride thermal springs in the northwest. All hot springs examined are used mainly for health purposes in Iran. Acanthamoeba were identified by both morphology and PCR (polymerase chain ...
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Genotypic variation in peanut for transpiration response to vapor pressure deficit
Conservation of soil water resulting from decreases in stomata conductance under atmospheric high vapor pressure deficit (VPD) conditions is a possible approach for enhanced tolerance of water deficit by crops. Water deficit is usually a concern in peanut (Arachis hypogea L.) since it is frequently grown on sandy soils with low water-holding capacity. Seventeen peanut genotypes were studied to ...
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Genotyping of
Acanthamoeba isolated from water in recreational areas of Tehran, IranA comprehensive survey assessing the presence of Acanthamoeba was conducted on 50 samples from water sources in parks and public squares from 22 municipal districts of Tehran, Iran. The prevalence and genotypes of Acanthamoeba were determined by PCR and the PCR fragments of ribosomal RNA genes sequenced. Sixteen (32%) samples were positive for Acanthamoeba spp. Sequence analysis revealed that the ...
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Genotypic variation in epidermal conductance and its associated traits among oryza sativa and o. glaberrima cultivars and their interspecific progenies
Epidermal conductance (g.min) is a measurement of water loss through the cuticle and stomata when stomatal conductance is minimal. Two pot experiments were conducted to evaluate genotypic variation in gmin for 10 to 12 rice cultivars (Oryza spp.) grown under flooded conditions. Epidermal conductance was estimated by measuring loss of water from the excised topmost fully-expanded leaves. ...
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Diversity and relatedness of common carpetgrass germplasm
Common carpetgrass (Axonopus fissifolius Raddi) is a stoloniferous, sod-forming species used sparingly as a turfgrass. No information is available regarding the relationships between germplasm accessions of this species. Accordingly, the initial objective of this study was to evaluate the relatedness of common carpetgrass germplasm for morphological and turfgrass performance traits. This was ...
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Contaminant driven genetic erosion: A case study with Daphnia longispina
Natural populations exposed to pollutants are predicted to experience a loss of genetic diversity, especially through genetic drift, gene flow (emigration), and/or selection (as sensitive genotypes may be lost). In the present study, the authors discuss the use of selectable markers and neutral markers to evaluate a contaminant‐driven loss of genetic diversity and possible implications of genetic ...
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Alterations in photosynthetic pigments, protein and osmotic components in cotton genotypes subjected to short-term drought stress followed by recovery
In order to assess drought tolerance mechanism in cotton, short-term drought-induced biochemical responses were monitored in two cotton (Gossypium hirsutum L.) genotypes contrasting their tolerance to water deficit. The seeds of two genotypes, namely GM 090304 (moderately drought tolerant) and Ca/H 631 (drought sensitive), were sown in pots containing soil, sand and peat in the ratio of 1:1:1, ...
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Detection of assemblages A and B of Giardia duodenalis in water and sewage from São Paulo state, Brazil
Giardia duodenalis is a protozoan that parasitizes humans and other mammals and causes giardiasis. Although its isolates have been divided into seven assemblages, named A to G, only A and B have been detected in human faeces. Assemblage A isolates are commonly divided into two genotypes, AI and AII. Even though information about the presence of this protozoan in water and sewage is available in ...
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