HPLC Articles
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Ascentis™ C18 for the Analysis of Phenolic and Benzyl Compounds
Application Report 225 Phenolic and Benzyl Compounds Using Ascentis™ C18 This application demonstrates the suitability of Ascentis C18 for the efficient separation of phenolic and benzyl compounds by HPLC. Compounds analyzed include: Uracil, Phloroglucinol, Pyrogallol, Benzamide, Resorcinol, Catechol, Phenol, and Nitrobenzene. ...
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Analysis of Bisphenol A on Ascentis Express RP-Amide
Analysis of Bisphenol A and Related Compounds on Ascentis™ Express RP-Amide This application report shows the efficient separation of Bisphenol A, Dimethyl Bisphenol A, and Nonylphenol, via HPLC using an Ascentis Express RP-Amide column, under gradient conditions and with UV detection. ...
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Alternative Analysis of Formaldehyde-DNPH and Other Carbonyl-DNPH Derivatives by Capillary GC
Alternative Analysis of Formaldehyde-DNPH and Other Carbonyl-DNPH Derivatives by Capillary GC - GC analysis of 15 carbonyl-DNPH compounds has some advantages over the HPLC analyses described in US EPA and other environmental methods. Determination of formaldehyde and 14 other carbonyls in air according to US Environmental Protection Agency Method TO11 and American Society for Testing and ...
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HPLC prep - which filter fits your chromatography sample best?
Sample filtration protects your HPLC column and preserves data quality. Read our tips on filter and solvent selection and the use of tools to save time in HPLC prep, improve lab efficiency, and better your analytical chemistry techniques. Filtering samples prior to high-performance liquid chromatography (HPLC) analysis can help avoid frit clogging while maintaining data quality. Adding a ...
By Cytiva
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Analysis of Phenolic and Benzyl Compounds Using Ascentis™ RP-Amide
Application Report 226 Phenolic and Benzyl Compounds Using Ascentis™ RP-Amide This application demonstrates the suitability of Ascentis RP-Amide for the efficient separation of phenolic and benzyl compounds by HPLC, under isocratic conditions and with UV detection. Compounds analyzed include: Uracil, Phloroglucinol, Pyrogallol, Benzamide, Resorcinol, Catechol, Phenol, and Nitrobenzene. ...
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Microfiltration: Rocker VF12 Kit Saves Time and Money
Filtering organic solvents for applications such as HPLC buffer prep can be challenging in order to ensure chemical compatibility with filters (PVDF, Nylon, or PTFE), preserving longevity of an HPLC column, or containment of toxic fumes, but finding a suitable and comprehensive vacuum system is now easier than ever. Sterlitech is now offering a comprehensive benchtop vacuum system, the ...
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Application of HPLC Technology in Drug Detection
What is HPLC technology? High performance liquid chromatography (HPLC) is also called "high-speed liquid chromatography", "high-resolution liquid chromatography", "modern column chromatography" and so on. High performance liquid chromatography is an important branch of chromatography. It uses liquid as the mobile phase, and uses a high-pressure injection system to pump single solvents with ...
By BOC Sciences
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Influence of environmental factors on degradation of carbendazim by Bacillus pumilus strain NY97-1
The influence of environmental factors on the performance of carbendazim degradation by Bacillus pumilus strain NY97-1 was investigated under aerobic conditions. The carbendazim degradation in culture medium was characterised by High-Performance Liquid Chromatography (HPLC) with UV detector. When the initial concentration of carbendazim was 10, 30, 50, 100, 300 mg/L, the degradation rate was ...
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Method Scaling in Preparative HPLC: Analytical Method & Preparative Method
VOC: “I tried to scale up my analytical HPLC method to prep and it didn’t work.” Andy: This is one of the most common things we hear from chemists wanting to do preparative chromatography. We also hear the following question pretty often too: VOC: “I have a good analytical HPLC method, how do I scale it to prep?” Andy: In this short blog, I will provide some of ...
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Waters HPLC & UHPLC Application Notes at Your Finger Tips
Summary A quick reference notebook to Waters HPLC/UHPLC application note highlights. 140 application notes covering a wide range of markets, such as Pharma, Food, and Health Science. Condensed one page format highlighting experimental conditions and chromatographic results. Tips on how to choose the best column to deliver throughput, resolution, and efficiency for your next ...
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Toxic Microcystis aeruginosa in Lake Velencei
Abstract: Lake Velencei is a shallow lake in Hungary. The first cyanobacterial bloom of Microcystis aeruginosaappeared in the lake in 1991. This cyanobacterial species was also found earlier but blooms were not recorded. The toxins of M. aeruginosawere studied in Lake Velencei in 1992. The present study deals with the toxicity of M. aeruginosabiomass of different origin detected by ...
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ACQUITY Arc System: Transferring Validated HPLC Methods within USP Guidelines
The thought of transferring established high performance liquid chromatography (HPLC) methods onto a another vendor’s LC, or a new platform like UHPLC/UPLC, may cause a bit of anxiety for those that may be unaware of how easy it can be. The reason that the topic of analytical method transfer induces so much angst is that for the transfer of an LC method to succeed, the method has to work ...
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Comparison of the photoconversion of para-chlorophenol under simulated sunlight and UV irradiation in ice
The photochemistry of para-chlorophenol (4-CP) was studied under simulated sunlight (λ > 300 nm) and UV irradiation by using a 125 W high-pressure mercury lamp with or without a hard glass as light source in an ice matrix. The experiments were carried out in a photochemical cold chamber reactor at −14 to −12 °C. The photoconversion rate, photoproducts and photoconversion mechanism of 4-CP were ...
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What is the pressure limit for my LC column?
This is a question we get in Tech Services fairly often. Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ROC LC columns, the maximum operating pressure is 400 Bar/ 5800 psi, which is also the limit for older or more conventional HPLC systems and close to the ...
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What is electrochemical detection and what are its applications?
Electrochemical detection (ECD) for HPLC or UHPLC / UPLC is an extremely selective and sensitive detection technique that is applied in several analyses such as the neurotransmitters dopamine, serotonin and noradrenalin, or analytes such as 5HIAA, catecholamines and metanephrines. ECD has an enormous linear dynamic range of more than 6 orders of magnitude. This means that concentrations can be ...
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Advances in Bromine Speciation by HPLC/ICP-MS
Bromine is a natural component found in waters, most commonly as the bromide ion, Br-. A common procedure for purifying drinking waters is treatment with ozone to kill bacteria. A byproduct of ozonolysis is the conversion of bromide to bromate (BrO3-), a known carcinogen. Therefore, a need exists to measure both bromide and bromate in drinking waters, as opposed to total bromine content. Our ...
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Cannabis quality tests now much faster thanks to HPLC
A new method of measuring phytocannabinoids has been developed and HPLC has been found to hold the key to faster measurements times. Read on to learn more and to discover our nitrogen generators for HPLC – ELSD. Researchers from the University of British Columbia have created a new method of measuring phytocannabinoids which is more than twice as quick as traditional methods. ...
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Indirectly suspended droplet microextraction of water‐miscible organic solvents by salting‐out effect for the determination of polycyclic aromatic hydrocarbons
A simple and low cost method “indirectly suspended droplet microextraction of water‐miscible organic solvents (ISDME)” by salting‐out effect prior to high performance liquid chromatography and UV detection (HPLC‐UV) was used for the determination of polycyclic aromatic hydrocarbons (PAHs) in different samples. ISDME is a combination of salting‐out extraction of water‐miscible organic solvent ...
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Reverse-phase HPLC method for the simultaneous analysis of triclosan and triclocarban in surface waters
A validated reverse-phase HPLC-DAD for the simultaneous determination of triclosan and triclocarban in surface water has been developed, because this method has not been used for determination of these disinfectant agents so far. An isocratic elution was achieved using a Develosil RP Aqueous AR-5 RP-30 column with a flow rate of 1.0 mL min−1. The mobile phase consisted of mixed methanol and water ...
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Arsenic and antimony speciation analysis in the environment using hyphenated techniques to inductively coupled plasma mass spectrometry: a review
Elemental speciation involves the separation and quantification of different oxidation states or chemical forms of a particular trace element. Trace metals exhibit widely different toxicities depending on their elemental species in the environment. On the other hand, inductively coupled plasma mass spectrometry (ICP-MS) is considered to be the method of choice for elemental analysis for several ...
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