hplc Articles

  • Carbonyl Sampling and Analysis by HPLC

    Sampling of Carbonyls Using a Dual-Layer LpDNPH Glass Sampling Tube and HPLC Analysis with an Ascentis Express C18 Column The dual-layer LpDNPH ORBO-555 tube is an effective sampler for measuring airborne carbonyl concentration. The low background levels in the sampler allow for accurate measurements of low concentration samples or samples collected in ...


    By Sigma-Aldrich Co., LLC

  • What can I use to filter my mobile phase?

    If you are using mobile phase that contains salts or, generally, any components that are weighed out for its preparation, you will need to filter the solution before using with HPLC or UHPLC. The most common type of filtration is membrane microfiltration. We sell an extensive group of products for this and they are discussed very well in a blog ...


    By Restek Corporation

  • Advances in Bromine Speciation by HPLC/ICP-MS

    Bromine is a natural component found in waters, most commonly as the bromide ion, Br-. A common procedure for purifying drinking waters is treatment with ozone to kill bacteria. A byproduct of ozonolysis is the conversion of bromide to bromate (BrO3-), a known carcinogen. Therefore, a need exists to measure both bromide and bromate in drinking waters, as opposed to total bromine content. Our ...


    By PerkinElmer, Inc.

  • HPLC separation and detection of bisphenol a (BPA) in plastics

    The BPA or bisphenol A (Figure 1) has become well know over the past year as concerns for its effect on human health and well being have been raised. The concerns over BPA began with baby bottles and spread to include other types of bottles. BPA is used in the production of two very common polymers PVC and Polycarbonate. PVC, Polyvinyl chloride, is used in many different products including ...


    By PerkinElmer, Inc.

  • Tips to maintain your HPLC & UHPLC systems and columns

    Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your ...


    By Waters Corporation

  • Determination of Additives and Preservatives in Beverages by Isocratic HPLC

    The Earth is 75% water, as are most life forms on Earth. People need to drink several quarts of water a day to live well, and most of us prefer to consume beverages other than water to satisfy our thirst. Soda (or Pop depending on your location) is the primary drink of the average American; and all Soda is manufactured (as opposed to coming out of the ground from a spring in Maine). As such, ...


    By Buck Scientific Inc

  • Bromate analysis analysis of bromate in drinking water by hplc and post-column derivatization

    Bromate is a disinfection by-product that is formed when Ozone reacts with naturally occurring Bromide in drinking water. Bromate is a known animal carcinogen and has also been listed as a group 2B toxin: probable human carcinogen. The U.S. EPA Method 300.1 employs conductivity as the means of detection which works well for most anions. However, the method is nonspecific and coeluting ...


    By Pickering Laboratories, Inc.

  • Monitoring Airborne Contaminants in Workplace Atmospheres, Using Sampling Devices and GC or HPLC

    Workers' exposure to hazardous substances must be held within acceptable limits. Therefore, airborne contaminant vapor and particles (including aerosols) in industrial atmospheres must be monitored. In the United States, the National Institute for Occupational Safety and Health (NIOSH) and the Occupational Safety and Health Administration (OSHA) offer, for many such substances, specific ...


    By Sigma-Aldrich Co., LLC

  • Measurement of Explosive Residues from Munitions and Armaments by Isocratic HPLC

    The government and the military have spent millions of dollars on making millions of tons of explosive materials. Most of these materials are warehoused in storage depots and often-present problems with leaking containers and contamination of the surrounding environment. Some are still used for testing and need to be checked for residual contamination and combustion efficiency. The industrial ...


    By Buck Scientific Inc

  • Speciation of five arsenic compounds in urine by HPLC/ICP-MS

    High-performance liquid chromatography (HPLC) coupled to an ICP-MS has become an invaluable analytical tool for the determinations of trace levels of individual arsenic compounds (speciation). Speciation of arsenic is used to improve our knowledge of arsenic’s biochemical interactions and to evaluate toxicity risks. The toxicity and bioavailability of arsenic is highly dependent on form or ...


    By PerkinElmer, Inc.

  • Solid Phase Extraction of Pesticides from Fruits and Vegetables, for Analysis by GC or HPLC

    Recovery rates for polar analytes, including carbamate and thiourea pesticides, are higher and less variable with ENVI-Carb carbon-based solid phase extraction tubes than with C8- or C18- silica-based tubes. Independent investigators found ENVI-Carb tubes to be an effective part of sample cleanup for more than 200 organochlorine, organophosphorus, nitrogen-containing, and carbamate pesticides, ...


    By Sigma-Aldrich Co., LLC

  • SPE Cleanup of Wheat Extracts Prior to the HPLC Analysis of the Mycotoxin Deoxynivalenol

    Deoxynivalenol (DON) is a mycotoxin produced by the Fusarium fungal species graminearum and culmorum. These Fusariumfungi infect cereal crops primarily in wet conditions when temperatures range from 15–30 °C, resulting in grain head damage and DON contamination ...


    By Sigma-Aldrich Co., LLC

  • Reverse-phase HPLC method for the simultaneous analysis of triclosan and triclocarban in surface waters

    A validated reverse-phase HPLC-DAD for the simultaneous determination of triclosan and triclocarban in surface water has been developed, because this method has not been used for determination of these disinfectant agents so far. An isocratic elution was achieved using a Develosil RP Aqueous AR-5 RP-30 column with a flow rate of 1.0 mL min−1. The mobile phase consisted of mixed methanol and water ...


    By IWA Publishing

  • Analysis of Organic plating Bath Additives and Chemicals by Isocratic HPLC

    In today’s world, over 75% of all metal surfaces on manufactured metal “items” have some sort of plated finish on them. From highchromium steel car bumpers to zinc diecast toys to Silver-contact electronic components. While the primary composition of these plating baths are inorganic (the metals and salts that are used to make up the desired metal finish), almost all types of plating baths; from ...


    By Buck Scientific Inc

  • Analysis of atrazine and its degradation products in loamy soil by SPE and HPLC

    The degradation and leaching of atrazine and its degradation products (hydroxyatrazine, desethylatrazine, deisopropylatarzine and didealkylatrazine) were studied into loamy soil. An agriculture soil field (50 × 50 sq. feet with brinjal plants) was selected and atrazine spray was carried out once in a year. The irrigation of this plot was carried out once in a month. The sampling of soil was ...


    By Inderscience Publishers

  • Method Scaling in Preparative HPLC: Analytical Method & Preparative Method

    VOC:  “I tried to scale up my analytical HPLC method to prep and it didn’t work.” Andy:  This is one of the most common things we hear from chemists wanting to do preparative chromatography. We also hear the following question pretty often too: VOC:  “I have a good analytical HPLC method, how do I scale it to ...


    By Waters Corporation

  • ACQUITY Arc System: Transferring Validated HPLC Methods within USP Guidelines

    The thought of transferring established high performance liquid chromatography (HPLC) methods onto a another vendor’s LC, or a new platform like UHPLC/UPLC, may cause a bit of anxiety for those that may be unaware of how easy it can be. The reason that the topic of analytical method transfer induces so much angst is that for the transfer of an LC method to succeed, the method has to work as ...


    By Waters Corporation

  • Rapid HPLC Analysis of PAH Compounds, Using Porous 3µm Particles

    Polynuclear aromatic hydrocarbons (PAHs) are a severe environmental hazard with a variety of sources. Airborne particles, combustion products, and fossil fuels all contain these toxic aromatic substances. Analysis by gas chromatography (GC) has been problematic because GC phases do not meet the need for shape selectivity in separating PAHs. Conversely, high-density C18 octadecylsilane (ODS) ...


    By Sigma-Aldrich Co., LLC

  • Development of a Cleaning Validation Method for MICRO® A07 Citric Acid Cleaner Using HPLC-CAD

    Abstract A high performance liquid chromatography (HPLC) method with a Corona Charged Aerosol detector for the cleaning validation of MICRO* A07 Citric Acid Cleaner residue has been developed. The pooled average repeatability of 3 replicates at 6 concentration levels expressed as RSD was 1.3 % while the pooled average intermediate precision (intra-day repeatability in 3 days) of 3 replicates ...

  • Sulfonamide artificial sweeteners in food products analysis of acesulfame, saccharin and cyclamate by HPLC with post-column derivatization

    Nonnutritive sweeteners are widely used in foods and beverages. Since some studies raised questions about long-term safety of these compounds, their concentration in food products is regulated. Among the artificial sweeteners used worldwide Saccharin and Cyclamate cause most controversy and their use is restricted in many countries, including the US. Liquid chromatography is a method of choice ...


    By Pickering Laboratories, Inc.

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