hplc flow Articles

  • Tips to maintain your HPLC & UHPLC systems and columns

    Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your ...


    By Waters Corporation

  • Reverse-phase HPLC method for the simultaneous analysis of triclosan and triclocarban in surface waters

    A validated reverse-phase HPLC-DAD for the simultaneous determination of triclosan and triclocarban in surface water has been developed, because this method has not been used for determination of these disinfectant agents so far. An isocratic elution was achieved using a Develosil RP Aqueous AR-5 RP-30 column with a flow rate of 1.0 mL min−1. The mobile phase consisted of mixed methanol and water ...


    By IWA Publishing

  • ACQUITY Arc System: Transferring Validated HPLC Methods within USP Guidelines

    The thought of transferring established high performance liquid chromatography (HPLC) methods onto a another vendor’s LC, or a new platform like UHPLC/UPLC, may cause a bit of anxiety for those that may be unaware of how easy it can be. The reason that the topic of analytical method transfer induces so much angst is that for the transfer of an LC method to succeed, the method has to work as ...


    By Waters Corporation

  • SPE Cleanup of Wheat Extracts Prior to the HPLC Analysis of the Mycotoxin Deoxynivalenol

    Deoxynivalenol (DON) is a mycotoxin produced by the Fusarium fungal species graminearum and culmorum. These Fusariumfungi infect cereal crops primarily in wet conditions when temperatures range from 15–30 °C, resulting in grain head damage and DON contamination ...


    By Sigma-Aldrich Co., LLC

  • Method Scaling in Preparative HPLC: Analytical Method & Preparative Method

    VOC:  “I tried to scale up my analytical HPLC method to prep and it didn’t work.” Andy:  This is one of the most common things we hear from chemists wanting to do preparative chromatography. We also hear the following question pretty often too: VOC:  “I have a good analytical HPLC method, how do I scale it to ...


    By Waters Corporation

  • What is the pressure limit for my LC column?

    This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ...


    By Restek Corporation

  • Chromatography Tips

    No.1 Gas Chromatography Tips  Our Founders and engineers of the company had accumulated more than 60 years experience in the research and development of HPLC instrumentation; HPLC column technology; mass spectrometer installation, training and services; chemical and biochemical separation, detection and quantification, Proteomics and cancer biomarker research and ...


    By AcuTech Scientific Inc.

  • Electrochemical degradation of methyl parathion

    This paper describes the degradation of commercially available methyl parathion by electro oxidation using Ti/RuO2 as anode, stainless steel as cathode and sodium chloride as a supporting electrolyte. Experiments were carried out at constant flow rate and various current densities such as 10, 12 and 15 A/dm² and various concentrations of sodium chloride. At regular time ...


    By Inderscience Publishers

  • Determination of chlorpyrifos residues in buffalo meat samples using high performance liquid chromatography

    Chlorpyrifos, an organophosphorous compound, is widely used to improve the yield of agricultural produce in India. Public concern over pesticide residues in foods has been increasing such that it has become a significant food safety issue. The present study was conducted to screen 254 buffalo meat samples collected from different slaughter houses for chlorpyrifos residues. The putative ...


    By Inderscience Publishers

  • Out with the old, in with the new…

    We’re all familiar with the adage, “Out with the old, in with the new,” but we probably tend to think of it as it applies to clothes for donation or past relationships, and maybe not in terms of updating our old laboratory equipment. After attending my first WCBP conference, held in Washington, D.C. during January, and hearing life cycle management and ICH Q12 being discussed ...


    By Waters Corporation

  • Calibration of a passive, in situ, integrative sampler for monitoring of microbial biotoxins in aquatic environments

    In this work, an integrative passive sampler based on a silicone membrane filled with a suspension of γ-Fe2O3 at pH 3.5 was developed. The novel device was calibrated for the measurement of microcystin concentrations in water. Laboratory calibration studies of the passive sampling devises under controlled conditions of temperature, water turbulence, and analyte concentration were ...


    By IWA Publishing

  • Comparison of different anion-exchange chromatography resins for the purification of cyanobacterial microcystins

    For the first time, different types of diethylaminoethyl (DEAE) anion-exchange resins, widely used in previous studies, were investigated to determine the most effective resin for the purification of microcystins (MCs). MCs were extracted from freeze-dried Microcystis aeruginosa cells that had been harvested from the Bueng Nong Khot reservoir, Khon Kaen, Thailand. The toxins were precipitated ...


    By IWA Publishing

  • The Advantages of a Targeted 2D LC Approach

    Webinar Highlights | Focus on the Relevant: The Advantages of a Targeted 2D Chromatography Approach Hi folks, I’m Daniel Root, Senior Product Marketing Manager for Multidimensional LC Systems at Waters. I recently conducted a webinar that addressed multidimensional liquid chromatography – an exceptional tool for the improvement of characterization and separation of ...


    By Waters Corporation

  • Glyphosate and AMPA in drinking water

    Carcinogenic or not? The broad-spectrum herbicide glyphosate is used all over the world in agriculture. Alongside farming, the chemical is also used for weed-killing in domestic gardens and in public and private spaces kept free from «vegetal invasion», such as railway tracks. Glyphosate has been used since the 1970s in pesticides and was hitherto thought to be harmless at ...


    By Metrohm AG

  • High-throughput Microscale Bioanalysis is not a Paradox

    Question: When someone says microscale chromatography, what comes to mind? Increased sensitivity? Reduced sample volume? Enhanced MS sampling efficiency? Yes, yes, and yes! You might also say, “finicky,” “slow,” or “only one person in our group knows how to use it!” Maybe even, “there’s no way I can successfully transfer that method, so why ...


    By Waters Corporation

  • Thermo Scientific connects at GPSG Brazil delivers enterprise-level productivity linking laboratories to ERP - case study

    The Global Pharmaceutical Supply Group (GPSG) Brazil is an APILA unit of Janssen-Cilag Farmacêutica Ltda., (www.janssen-cilag.com.br) located in São Paulo, Brazil. Janssen-Cilag is one of the world’s leading research-based pharmaceutical companies specializing in pain management and fungal disease. Janssen-Cilag is a member of the Johnson & Johnson family of companies; the ...

  • Better tools for fast and convenient Patulin analysis

    Analytical Standards and New SupelMIP SPE Cartridges The mycotoxin patulin is often found in rotten apples. It is mostly produced by Aspergillus, Penicillium and Byssochlamys and shows a variety of short-term and long-term effects in animal studies, ranging from gastrointestinal problems and neurotoxicity to genotoxicity and teratogenicity. Therefore, many countries set limits for patulin ...


    By Sigma-Aldrich Co., LLC

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