hplc instrument Articles

  • Tips to maintain your HPLC & UHPLC systems and columns

    Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your ...


    By Waters Corporation

  • ACQUITY Arc System: Transferring Validated HPLC Methods within USP Guidelines

    The thought of transferring established high performance liquid chromatography (HPLC) methods onto a another vendor’s LC, or a new platform like UHPLC/UPLC, may cause a bit of anxiety for those that may be unaware of how easy it can be. The reason that the topic of analytical method transfer induces so much angst is that for the transfer of an LC method to succeed, the method has to work as ...


    By Waters Corporation

  • High-Performance Liquid Chromatography for Determination of N-Nitrosodimethylamine in Water

    High-performance liquid chromatography (HPLC) was applied for aqueous determination of Nnitrosodimethylamine (NDMA). A fluorescence derivatization technique was adopted to enhance detection using HPLC. Derivatization with dansyl chloride was optimized for excitation and emission wavelengths (340 and 530 nm, respectively), pH (9-12), and concentration of dansyl chloride (500 mg/L). An instrumental ...

  • 70-min amino acid analysis of physiological samples

    Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. Post-column derivatization with Ninhydrin offers unmatched selectivity and reproducibility of the analysis for most challenging matrixes. Pinnacle PCX post-column derivatization system allows analysts to combine eluant gradient capabilities of modern HPLC instruments with column ...


    By Pickering Laboratories, Inc.

  • Low Level Determination of Polynuclear Aromatic Hydrocarbons in Environmental Samples, Using GC/MS

    Low level analysis of PAHs is required to attain various clean-up limits for environmental regulations. The ability to maximize GC/MS instrument conditions to meet lower reporting levels results in a more efficient and reliable determination of these analytes. The following analysis provides the necessary sensitivity and selectivity. The procedure requires that the parameters for GC as well as MS ...


    By Sigma-Aldrich Co., LLC

  • What is the pressure limit for my LC column?

    This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ...


    By Restek Corporation

  • The development of cation-exchange chromatography in free amino acids analysis

    Although it was not called a liquid chromatograph, the first such instrument was introduced in late 1959 by Beckman Instruments and was designated the M.S. Amino Acid Analyzer in honour of Moore and Stein. Inject valves had yet to be invented, the buffer switching valves were glass stopcocks and the column temperature was controlled by circulating water baths. Two positive displacement pumps ...


    By Pickering Laboratories, Inc.

  • 30-minute amino acid analysis of hydrolyzed samples

    Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. No other tech¬nique has been shown to match the reproducibility and selectivity of ion-exchange analysis with post-column Ninhydrin detection. Nor is there a chromatography technique that provides as much information; the 570/440 nm signal ratio for each amino acid is a ...


    By Pickering Laboratories, Inc.

  • Pinnacle PCX Carbamate Testing at Nebraska Health

    Beta Site Evaluation of the EPA Carbamate in Drinking Water Method 531.1 with Pickering Laboratories Pinnacle PCX Integrated LC/Post-Column Derivatization Instrument. The Nebraska DHHS Public Health Environmental Laboratory conducts environmental testing for the state of Nebraska. It employs mandated U.S. Environmental Protection Agency (EPA) methods for the determination of regulated substances ...


    By Pickering Laboratories, Inc.

  • A bridge with our customers - Prominence-i (i-Series) - Case Study

    Anthem Biosciences, which is ranked among the top 5 leading Contract Research Organisations (CROs) in India, is emerging as a global leader in the domain of Integrated Pharmaceuticals business. Anthem, which calls itself a Contract Research and Innovation Service Provider (CRISP), offers a whole gamut of services dedicated to enabling and sustaining global research efforts in the discovery of new ...

  • Chromatography Tips

    No.1 Gas Chromatography Tips  Our Founders and engineers of the company had accumulated more than 60 years experience in the research and development of HPLC instrumentation; HPLC column technology; mass spectrometer installation, training and services; chemical and biochemical separation, detection and quantification, Proteomics and cancer biomarker research and ...


    By AcuTech Scientific Inc.

  • Rapid raw material identification for formulation compounds using handheld Raman technology

    Ever since the European Medicines Agency (EMA) recommended that all pharmaceutical companies test every raw material that passes through their manufacturing facilities, this practice has attracted great interest in the nutraceuticals, food manufacturing, cosmetics, and agricultural industries, pushing them to strive for more rigorous and technology-oriented quality control standards. In recent ...


    By B&W Tek

  • Materials Testing Laboratory Provides Cutting Edge Contamination Testing Solutions

    Many industries have extremely tight parameters for any contamination of parts that are used in their manufacturing process.  Testing for contamination and quality control is an integral part of these companies’ final products. At EMSL Analytical, the Materials Testing Laboratory has extensive experience in providing highly detailed analytical services to assist manufacturers in ...


    By EMSL Analytical, Inc.

  • Simon Fraser University Opens Suite of Mass Spectrometers for Natural Products Discovery Research

    As a graduate student at the University of British Columbia, Dr. Roger Linington discovered his future life’s work serendipitously. While studying sponges, it became clear to him that one of the challenges in studying macroorganisms is their limited or slowly replenishing supply. To perform mechanism and mode-of-action studies, he needed a larger, more accessible source of ...


    By Waters Corporation

  • Matrix LIMS configured and implemented within 3 months case study

    Laboratory staff at Glatfelter have established an extremely close, ongoing working relationship with Autoscribe. This has allowed the design and implementation in just 3 months of a configured Matrix Gemini LIMS for the company's corporate analytical services group. Organisation Established since 1864, global paper supplier Glatfelter has expanded its product range from high-quality ...


    By Autoscribe Informatics

  • USLC columns put the right tools in your LC method development toolbox

    Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection The most significant influence on chromatographic peak separation, or resolution, is column selectivity. Unfortunately, column selectivity is also the least understood and most underutilized parameter. To improve selectivity, method developers often concentrate on ...


    By Restek Corporation

  • Glyphosate and AMPA in drinking water

    Carcinogenic or not? The broad-spectrum herbicide glyphosate is used all over the world in agriculture. Alongside farming, the chemical is also used for weed-killing in domestic gardens and in public and private spaces kept free from «vegetal invasion», such as railway tracks. Glyphosate has been used since the 1970s in pesticides and was hitherto thought to be harmless at ...


    By Metrohm AG

  • LC/MS/MS analysis of metabolites of synthetic Cannabinoids JWH-018 and JWH-073 in Urine

    Abstract A liquid chromatographic method was developed to resolve a comprehensive set of metabolites of JWH-018 and JWH-073. In addition to the chromatographic analysis method, an extraction method was developed to recover a broad range of synthetic cannabinoid metabolites, including carboxylic acid metabolites that are not traditionally recovered using a high pH liquid/liquid extraction. ...


    By Restek Corporation

  • High-throughput Microscale Bioanalysis is not a Paradox

    Question: When someone says microscale chromatography, what comes to mind? Increased sensitivity? Reduced sample volume? Enhanced MS sampling efficiency? Yes, yes, and yes! You might also say, “finicky,” “slow,” or “only one person in our group knows how to use it!” Maybe even, “there’s no way I can successfully transfer that method, so why ...


    By Waters Corporation

  • Thermo Scientific connects at GPSG Brazil delivers enterprise-level productivity linking laboratories to ERP - case study

    The Global Pharmaceutical Supply Group (GPSG) Brazil is an APILA unit of Janssen-Cilag Farmacêutica Ltda., (www.janssen-cilag.com.br) located in São Paulo, Brazil. Janssen-Cilag is one of the world’s leading research-based pharmaceutical companies specializing in pain management and fungal disease. Janssen-Cilag is a member of the Johnson & Johnson family of companies; the ...

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