HPLC solvent Articles

  • Tips to maintain your HPLC & UHPLC systems and columns

    Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your ...


    By Waters Corporation

  • Microfiltration: Rocker VF12 Kit Saves Time and Money

    Filtering organic solvents for applications such as HPLC buffer prep can be challenging in order to ensure chemical compatibility with filters (PVDF, Nylon, or PTFE), preserving longevity of an HPLC ...


    By Sterlitech Corporation

  • Indirectly suspended droplet microextraction of water‐miscible organic solvents by salting‐out effect for the determination of polycyclic aromatic hydrocarbons

    A simple and low cost method “indirectly suspended droplet microextraction of water‐miscible organic solvents (ISDME)” by salting‐out effect prior to high performance liquid chromatography and UV detection (HPLC‐UV) was used for the determination of polycyclic aromatic hydrocarbons (PAHs) in different samples. ISDME is a combination of salting‐out extraction of water‐miscible organic solvent ...


    By John Wiley & Sons, Ltd.

  • Chromatography Tips

    No.1 Gas Chromatography Tips  Our Founders and engineers of the company had accumulated more than 60 years experience in the research and development of HPLC instrumentation; HPLC column technology; mass spectrometer installation, training and services; chemical and biochemical separation, detection and quantification, Proteomics and cancer biomarker research and ...


    By AcuTech Scientific Inc.

  • What is the pressure limit for my LC column?

    This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ...


    By Restek Corporation

  • News on Air 2013, Volume 4: Labware and Reagents

    Welcome to volume 4 of our News on Air bulletin. This edition focusses on some of the labware and reagents that are used in this challenging area of analysis. Success in air monitoring quantitation requires that all the equipment and reagents used are inherently low in background concentrations, to prevent false positives. Commonly used materials may include: Filters, membranes, and ...


    By MilliporeSigma

  • Solid-phase extraction of room-temperature imidazolium ionic liquids

    Abstract Owing to their favorable properties, ionic liquids have recently gained recognition as possibly environmentally benign solvents. Now among the most promising industrial chemicals, they have already been labeled 'green' but this appellation seems due entirely to their very low vapor pressure. This growing interest in the various applications of ionic liquids will soon result in ...


    By Springer

  • News on Air 2013, Volume 1: Products for Sampling

    For decades, Sigma-Aldrich has been a key supplier of innovative & high technology air monitoring products for various areas of gas/air sampling. Enclosed, you will find an overview on new and interesting devices to improve or simplify your air sampling. Supelco and Sigma-Aldrich offer a range of active and passive air sampling products, in addition to various analytical chemistry tools ...


    By MilliporeSigma

  • Clean your test sieves to maintain accurate results

    A standard process in analytical laboratories is sieve analysis, the practice of accessing the particle size distribution of a granular material. The trouble is, sieves prove quite the challenge to clean thoroughly enough to get all the contaminants out of the mesh. Every last grain stuck in the sieve must be removed to gain accurate analysis. A solution has been found in the use ...


    By Camlab Limited

  • Hollow-fiber-supported liquid-phase microextraction using an ionic liquid as the extractant for the pre-concentration of bisphenol A, 17-β-estradiol, estrone and diethylstilbestrol from water samples with HPLC detection

    A new method for the determination of four endocrine disrupting compounds (EDCs) (bisphenol A, 17-β-estradiol, estrone and diethylstilbestrol) in water samples has been developed using polypropylene hollow-fiber-supported ionic liquid (IL, 1-Octyl-3-methylimidazolium hexafluorophosphate, [C8MIM][PF6]) microextraction [HF-liquid-phase microextraction (LPME)] combined with ...


    By IWA Publishing

  • What can I use to filter my mobile phase?

    If you are using mobile phase that contains salts or, generally, any components that are weighed out for its preparation, you will need to filter the solution before using with HPLC or UHPLC. The most common type of filtration is membrane microfiltration. We sell an extensive group of products for this and they are discussed very well in a blog ...


    By Restek Corporation

  • The Clog Blog

    Clogging and over pressuring are definitely problematic for Liquid Chromatography columns. Back in 2005 John Dolan’s sage advice to chromatographers to make an LC column last forever was simple, “Never open the box!” As John pointed out, this is not practical and I have to agree. But as an LC column manufacturer I have seen remarks from customers regarding clogging as though it ...


    By Restek Corporation

  • High-throughput Microscale Bioanalysis is not a Paradox

    Question: When someone says microscale chromatography, what comes to mind? Increased sensitivity? Reduced sample volume? Enhanced MS sampling efficiency? Yes, yes, and yes! You might also say, “finicky,” “slow,” or “only one person in our group knows how to use it!” Maybe even, “there’s no way I can successfully transfer that method, so why ...


    By Waters Corporation

  • SPE Cleanup of Wheat Extracts Prior to the HPLC Analysis of the Mycotoxin Deoxynivalenol

    Deoxynivalenol (DON) is a mycotoxin produced by the Fusarium fungal species graminearum and culmorum. These Fusariumfungi infect cereal crops primarily in wet conditions when temperatures range from 15–30 °C, resulting in grain head damage and DON contamination ...


    By MilliporeSigma

  • Webinar Recap: “Developing, Testing, and Troubleshooting Chromatography Method Transfer”

    Hi, I’m Tom Wheat, Principal Scientist at Waters, and I recently hosted a webinar entitled, “Developing, Testing and Troubleshooting Chromatography Method Transfer.” Over the course of the webinar, we discussed the transfer of chromatographic methods that occurs at many stages in the development and production of a product. In its simplest form, the method is moved from one ...


    By Waters Corporation

  • LC/MS/MS analysis of metabolites of synthetic Cannabinoids JWH-018 and JWH-073 in Urine

    Abstract A liquid chromatographic method was developed to resolve a comprehensive set of metabolites of JWH-018 and JWH-073. In addition to the chromatographic analysis method, an extraction method was developed to recover a broad range of synthetic cannabinoid metabolites, including carboxylic acid metabolites that are not traditionally recovered using a high pH liquid/liquid extraction. ...


    By Restek Corporation

  • USLC columns put the right tools in your LC method development toolbox

    Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection The most significant influence on chromatographic peak separation, or resolution, is column selectivity. Unfortunately, column selectivity is also the least understood and most underutilized parameter. To improve selectivity, method developers often concentrate on ...


    By Restek Corporation

  • Analyze wine using ATR-FTIR spectroscopy | Spectroscopy Solutions

    Wine analysis using spectroscopy Within quality assurance and process monitoring practices, spectroscopy has been widely used for many years1. Increasingly spectroscopic techniques and advanced data processing are being adopted for authenticity testing.2 Such verification is particularly important in the wine industry, which provides a premium-priced ...


    By Specac Limited

  • Building a Perfect Purification Platform

    Dart NeuroScience is one of the largest privately owned biotech companies in San Diego with around 250 employees – and we have an interesting focus: memory. More specifically, our aim is to develop new technologies and therapies that can maintain or improve memory function and cognitive vitality. Much of our research is built on the work of ...


    By Waters Corporation

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