hplc system Articles

  • Tips to maintain your HPLC & UHPLC systems and columns

    Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your ...


    By Waters Corporation

  • Advances in Bromine Speciation by HPLC/ICP-MS

    Bromine is a natural component found in waters, most commonly as the bromide ion, Br-. A common procedure for purifying drinking waters is treatment with ozone to kill bacteria. A byproduct of ozonolysis is the conversion of bromide to bromate (BrO3-), a known carcinogen. Therefore, a need exists to measure both bromide and bromate in drinking waters, as opposed to total bromine content. Our ...


    By PerkinElmer, Inc.

  • ACQUITY Arc System: Transferring Validated HPLC Methods within USP Guidelines

    The thought of transferring established high performance liquid chromatography (HPLC) methods onto a another vendor’s LC, or a new platform like UHPLC/UPLC, may cause a bit of anxiety for those that may be unaware of how easy it can be. The reason that the topic of analytical method transfer induces so much angst is that for the transfer of an LC method to succeed, the method has to work as ...


    By Waters Corporation

  • Speciation of five arsenic compounds in urine by HPLC/ICP-MS

    High-performance liquid chromatography (HPLC) coupled to an ICP-MS has become an invaluable analytical tool for the determinations of trace levels of individual arsenic compounds (speciation). Speciation of arsenic is used to improve our knowledge of arsenic’s biochemical interactions and to evaluate toxicity risks. The toxicity and bioavailability of arsenic is highly dependent on form or ...


    By PerkinElmer, Inc.

  • Method Scaling in Preparative HPLC: Analytical Method & Preparative Method

    VOC:  “I tried to scale up my analytical HPLC method to prep and it didn’t work.” Andy:  This is one of the most common things we hear from chemists wanting to do preparative chromatography. We also hear the following question pretty often too: VOC:  “I have a good analytical HPLC method, how do I scale it to ...


    By Waters Corporation

  • Extraction of trace tilmicosin in real water samples using ionic liquid-based aqueous two-phase systems

    The effective method of ionic liquid-based aqueous two-phase extraction, which involves ionic liquid (IL) (1-butyl-3-methyllimidazolium chloride, [C4mim]Cl) and inorganic salt (K2HPO4) coupled with high-performance liquid chromatography (HPLC), has been used to extract trace tilmicosin in real water samples which were passed through a 0.45 μm filter. The effects of the ...


    By IWA Publishing

  • 70-min amino acid analysis of physiological samples

    Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. Post-column derivatization with Ninhydrin offers unmatched selectivity and reproducibility of the analysis for most challenging matrixes. Pinnacle PCX post-column derivatization system allows analysts to combine eluant gradient capabilities of modern HPLC instruments with column ...


    By Pickering Laboratories, Inc.

  • Determination of Perfluorinated Compounds (PFCs) in Soil and Sewage Sludge using the SpeedExtractor E-916

    Perfluorinated Compounds (PFCs) are fluorocarbons used in many commercial and domestic applications. They make materials stain, oil, and water resistant. They are persitent pollutants and are therefore monitored worldwide. Twelve PFC congeners were extracted from soil and sewage sludge samples using the SpeedExtractor E-916 and an ASE 200 system (Dionex). The PFC congeners were analyzed by ...


    By BÜCHI Labortechnik AG

  • What is the pressure limit for my LC column?

    This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ...


    By Restek Corporation

  • 30-minute amino acid analysis of hydrolyzed samples

    Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. No other tech¬nique has been shown to match the reproducibility and selectivity of ion-exchange analysis with post-column Ninhydrin detection. Nor is there a chromatography technique that provides as much information; the 570/440 nm signal ratio for each amino acid is a ...


    By Pickering Laboratories, Inc.

  • Paralytic shellfish toxins dinoflagelate-derived neurotoxins in bivalve mollusks

    Analysis Of Dinoflagelate Derived Neurotoxins In Bivalve Mullusks Using Hplc Postcolumn Fluorescence Method The paralytic shellfish toxins are a group of 18 secondary metabolites deposited in bivalve mollusks by dinoflagelates. Dinoflagelates blooms are seasonal, occurring during warm months. Since it is unpredictable whether an infestation will occur, the shellfish popula¬tion should be ...


    By Pickering Laboratories, Inc.

  • Pinnacle PCX Carbamate Testing at Nebraska Health

    Beta Site Evaluation of the EPA Carbamate in Drinking Water Method 531.1 with Pickering Laboratories Pinnacle PCX Integrated LC/Post-Column Derivatization Instrument. The Nebraska DHHS Public Health Environmental Laboratory conducts environmental testing for the state of Nebraska. It employs mandated U.S. Environmental Protection Agency (EPA) methods for the determination of regulated substances ...


    By Pickering Laboratories, Inc.

  • The development of cation-exchange chromatography in free amino acids analysis

    Although it was not called a liquid chromatograph, the first such instrument was introduced in late 1959 by Beckman Instruments and was designated the M.S. Amino Acid Analyzer in honour of Moore and Stein. Inject valves had yet to be invented, the buffer switching valves were glass stopcocks and the column temperature was controlled by circulating water baths. Two positive displacement pumps ...


    By Pickering Laboratories, Inc.

  • Chromatography Tips

    No.1 Gas Chromatography Tips  Our Founders and engineers of the company had accumulated more than 60 years experience in the research and development of HPLC instrumentation; HPLC column technology; mass spectrometer installation, training and services; chemical and biochemical separation, detection and quantification, Proteomics and cancer biomarker research and ...


    By AcuTech Scientific Inc.

  • o-Phthalaldehyde Reagent

    Introduction Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan under basic conditions. At a pH>9 and ambient temperature, reaction is generally complete within 30 seconds. The products of this reaction, 1-alkyl-2-alkylthio-substituted isoindoles, exhibit optimal excitation at 330 nm and maximal emission at 465 nm. The reagents ...


    By Pickering Laboratories, Inc.

  • Out with the old, in with the new…

    We’re all familiar with the adage, “Out with the old, in with the new,” but we probably tend to think of it as it applies to clothes for donation or past relationships, and maybe not in terms of updating our old laboratory equipment. After attending my first WCBP conference, held in Washington, D.C. during January, and hearing life cycle management and ICH Q12 being discussed ...


    By Waters Corporation

  • The fate of 14C-radiolabelled diclofenac and 4′-hydroxydiclofenac in membrane bioreactor treatment of wastewater

    This study aimed at enhancing knowledge on the fate of diclofenac (DF), together with its main human metabolite 4′-hydroxydiclofenac (4′OHDF), during wastewater treatment by using a laboratory-scale membrane bioreactor (MBR). The reactor was fed continuously with non-radiolabelled diclofenac for a one month period prior to a single pulse of a 14C-radiolabelled solution of DF and 4′OHDF. The ...


    By IWA Publishing

  • Comparison of simple ozonation and direct hydrogen peroxide processes in TNT removal from aqueous solution

    Oxidation of 2,4,6-trinitrotoluene (TNT) using simple ozonation and direct hydrogen peroxide was studied. An analytical method based on the HPLC (high performance liquid chromatography) system was used for TNT detection. The influence of parameters such as pH of the solution (3–10), initial TNT concentration (10–100 mg/L), dosage of ozone (0.2–1 g/hr), H2O2/TNT molar ratio (250–1,000), ...


    By IWA Publishing

  • Evaluation of removal efficiency of human antibiotics in wastewater treatment plants in Bangkok, Thailand

    This study aimed to investigate the antibiotic concentration at each stage of treatment and to evaluate the removal efficiency of antibiotics in different types of secondary and advanced treatment, as well as the effects of the location of their discharge points on the occurrence of antibiotics in surface water. Eight target antibiotics and four hospital wastewater treatment plants in Bangkok ...


    By IWA Publishing

  • Recycling of macronutrients from sea to land using mussel cultivation

    The presence of diarrheic shellfish toxins (DST) has been the main obstacle to mussel cultivation in Sweden. Monitoring of DST concentrations in mussels by HPLC for 12 years has shown great geographical and seasonal differences. Furthermore, individual mussels on the same farming strip may differ by a factor as great as 16. DST levels are usually low in summer, rise in September, remain high in ...


    By Inderscience Publishers

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