immunoassay Articles

  • Immunoassay specificity

    Specificity is an important characteristic of any laboratory test which describes its ability to distinguish between true (or specific) and non-specific results. With immunoassay methods, interferences which affect specificity can be categorized into two major classes: 1) those which affect the binding event between the antibody and an antigen in a general way, such as pH or ionic strength; or 2) ...


  • Quality control of immunoassays for pesticide residues

    I. Abstract Quality control of any analytical method is integral to its ability to produce reliable results. To adequately control a method, one must first understand the sources of variability. Some sources of error in an immunochemical method are similar to those of a traditional chromatographic technique. Immunoassays also present some unique potential error sources along with novel ...


  • Immunoassays for Environmental Contaminants (Pesticides) in Food and Water

    Immunoassays are one of the most powerful of all immunochemical techniques. They can be employed in a wide range of methods to detect large numbers of different compounds. In this paper, the principles of immunoassay will be explored in general terms. This will be followed by a more specific discussion of the application of these principles to the measurement of pesticide residues in food and ...


  • A case of enzyme immunoassay validation for the determination of clenbuterol residues in bovine urine

    Using the method of standard addition, a commercial EIA for qualitative screening of clenbuterol in bovine urine was validated. The method was robust on the influence of external factors. The recovery was 167.86% and CCβ 618.86 ng/L, and should be considered by presentation of quantitative results. Due to high crossreactivity, high responses are expected in test samples, contaminated with other ...


    By Inderscience Publishers

  • Detection of chlorpyrifos in air using low volume polyurethane foam (PUF) sampling with immunoassay

    MATRIX: Air samples ANALYTE: Chlorpyrifos RANGE OF DETECTION: 0.088 to 1.200 mg/m3 MATERIALS: Chlorpyrifos RaPID AssayÒ Kit and Sample Diluent. Reagents: methanol (pesticide grade). Equipment: 40 mL glass vials with Teflon lined caps, 13 x 100 mm glass test tubes, 5 mL and 20 mL serological pipets, micropipet ...


  • A SERS-based immunoassay for porcine circovirus type 2 using multi-branched gold nanoparticles

    Abstract We report on a facile immunoassay for porcine circovirus type 2 (PCV2) based on surface enhanced Raman scattering (SERS) using multi-branched gold nanoparticles (mb-AuNPs) as substrates. The mb-AuNPs in the immunosensor act as Raman reporters and were prepared via Tris base-induced reduction and subsequent reaction with p-mercaptobenzoic acid (pMBA). They possess good ...


    By AcuTech Scientific Inc.

  • Biomedical applications of organically modified bioconjugated silica nanoparticles

    Nanoparticles are one of the most important prominent areas in the field of nanotechnology, with a broad significant role in current life sciences and human healthcare applications. Among the nanoparticles silica nanoparticles are explored in many biological applications because their size dependent novel properties and functions differ drastically from those of the bulk materials. In biomedical ...


    By Inderscience Publishers

  • Infectious Disease Testing Industry : Strategic Assessments for Technologies & Applications of Leading Suppliers Analyzed in Report

    ReportsnReports.com adds report Infectious Disease Testing Market 2014: Strategic Assessments of Leading Suppliers to its store.This report provides detailed analyses of current and emerging technologies, and their potential applications for the infectious disease testing market, including molecular diagnostics, biochips, monoclonal antibodies, immunoassays, IT, gel microdroplets, ...

  • Matrix solid phase dispersion extraction of triazines from catfish tissues; examination of the effects of temperature and dissolved oxygen on the toxicity of atrazine

    A matrix solid-phase dispersion (MSPD) method to analyse the triazine herbicides atrazine, cyanazine, metribuzin and simazine in catfish muscle and liver is presented. An examination of the extract obtained in an immunoassay for triazines is also reported. The method is applied to the analysis of atrazine levels in catfish exposed to increasing levels of atrazine to determine toxicity to this ...


    By Inderscience Publishers

  • Interpretation of total petroleum hydrocarbon (TPH) results with RaPID assay kits

    SUMMARY The SDI RaPID Assays for petroleum hydrocarbon fuels (Total BTEX, PAHs) have been shown to provide excellent correlation with GC based (8015M) and IR based (418.1) methodologies for testing Total Petroleum Hydrocarbons (TPH). Linear relationships between immunoassay and TPH results with both gasoline and diesel fuels are demonstrated. Fuel reactivities with immunoassay are shown to ...


  • Use of PAHs RaPID ASSAY kits for semi-quantitative screening consistent with SW-846 Methood 4035

    BACKGROUND The Methods Section of the EPA Office of Solid Waste (OSW) has stated a need for more rapid, less expensive field screening procedures that do not compromise the accuracy of contaminant evaluation. The OSW screening approach incorporates a single point threshold or multiple individual point discriminators giving results in semiquantitative ranges lying between these points. SDI ...


  • Sample extraction kit user’s guide

    Intended Use This extraction kit is for use with the appropriate immunoassay test kit. Each Sample Extraction kit contains the materials necessary to process twelve (12) soil or wipe samples. Test Principles The reagents contained in the Sample Extraction kit have been optimized for fast, efficient removal of compounds from soil or surfaces and convenient ...


  • Use of SDI carcinogenic PAHs RaPID assay kits for semi-quantitative screening consistent with SW-846 method 4035

    BACKGROUND The Methods Section of the EPA Office of Solid Waste (OSW) has stated a need for more rapid, less expensive field screening procedures that do not compromise the accuracy of contaminant evaluation. The OSW screening approach incorporates a single point threshold or multiple individual point discriminators giving results in semiquantitative ranges lying between these points. SDI ...


  • Use of SDI total btex/tph rapid assay kits for semi-quantitative screening consistent with sw-846 method 4030

    BACKGROUND The Methods Section of the EPA Office of Solid Waste (OSW) has stated a need for more rapid, less expensive field screening procedures that do not compromise the accuracy of contaminant evaluation. The OSW screening approach incorporates a single point threshold or multiple individual point discriminators giving results in semiquantitative ranges lying between these points. SDI ...


  • Use of TNT RaPID ASSAY kits for semi-quantitative screening consistent with SW-846 method 4050

    BACKGROUND The Methods Section of the EPA Office of Solid Waste (OSW) has stated a need for more rapid, less expensive field screening procedures that do not compromise the accuracy of contaminant evaluation. The OSW screening approach incorporates a single point threshold or multiple individual point discriminators giving results in semiquantitative ranges lying between these points. SDI ...


  • Use of SDI 2,4-d rapid assay kits for semi-quantitative screening consistent with SW-846 METHOD 4015

    BACKGROUND The Methods Section of the EPA Office of Solid Waste (OSW) has stated a need for more rapid, less expensive field screening procedures that do not compromise the accuracy of contaminant evaluation. The OSW screening approach incorporates a single point threshold or multiple individual point discriminators and reports results in semi-quantitative ranges lying above, below or ...


  • Use of SDI pentachlorophenol rapid assay kits for semi-quantitative screening consistent with sw-846 method 4010

    BACKGROUND The Methods Section of the EPA Office of Solid Waste (OSW) has stated a need for more rapid, less expensive field screening procedures that do not compromise the accuracy of contaminant evaluation. The OSW screening approach incorporates a single point threshold or multiple individual point discriminators and reports results in semi-quantitative ranges lying above, below or ...


  • Use of PCB RaPID ASSAY kits for semi-quantitative screening consistent with SW-846 method 4020

    BACKGROUND The Methods Section of the EPA Office of Solid Waste (OSW) has stated a need for more rapid, less expensive field screening procedures that do not compromise the accuracy of contaminant evaluation. The OSW screening approach incorporates a single point threshold or multiple individual point discriminators giving results in semiquantitative ranges lying between these points. SDI ...


  • AuNP‐based colorimetric aptasensor for rapid detection of six organophosphorus pesticides

    Fast immunoassay‐based screening methods are unavailable for most of small‐molecule pesticides due to lack of immunogenicity and the difficulty in obtaining antibodies by animal immunization. Aptamers are single‐stranded DNAs selected through an in vitro process, which can bind to any target including non‐immunogenic small molecules with high affinity and specificity. Though various ...


    By John Wiley & Sons, Ltd.

  • Correction of non-specific binding in RaPID assays

    Heterogeneous immunoassays are typically subject to a low level of direct binding of enzyme-antigen conjugate to the solid support surface. This binding results in a very small amount of positive bias. Users can correct for this bias by using the procedure presented below. Standard curves which serve to estimate pesticide (antigen) concentration in immunoassays are usually sigmodial. By using ...


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