laboratory scale sequencing batch reactor Articles
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The kinetics for ammonium and nitrite oxidation under the effect of hydroxylamine
The kinetics for ammonium (NH4+) oxidation and nitrite (NO2−) oxidation under the effect of hydroxylamine (NH2OH) were studied by respirometry using the nitrifying sludge from a laboratory-scale sequencing batch reactor. Modified models were used to estimate kinetics parameters of ammonia and nitrite oxidation under the effect of hydroxylamine. An inhibition effect of hydroxylamine on the ...
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Effect of cycle period and substrate composition on phosphorous removal potential of a laboratory-scale sequencing batch reactor
The performance of a laboratory-scale sequencing batch reactor (SBR) of 20-litre capacity was evaluated for simultaneous removal of soluble COD (SCOD), NH4+?N and phosphorus by changing the compositions and concentrations of substrates under different combinations of REACT period. The total cycle period of 10 h comprised 0.5 h FILL period, 8 h REACT period, 1 h SETTLE period and 0.5 h IDLE/DRAW ...
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Organic carbon effect on nitrifying bacteria in a mixed culture
This study investigated the effect of organic carbon source on ammonia oxidizing community in single sludge laboratory scale sequencing batch reactors (SBR). Two sequencing batch reactors performing simultaneous carbon oxidation and nitrification were operated. Operationally and functionally, these two reactors were identical, except that one reactor was fed peptone and sodium acetate, and the ...
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The sequencing batch reactor as an excellent configuration to treat wastewater from the petrochemical industry
In the present study, the influence of a changing feeding pattern from continuous to pulse feeding on the characteristics of activated sludge was investigated with a wastewater from the petrochemical industry from the harbour of Antwerp. Continuous seed sludge, adapted to the industrial wastewater, was used to start up three laboratory-scale sequencing batch reactors. After an adaptation ...
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Partial nitrification of sludge reject water by means of aerobic granulation
Granular sludge formation was performed in a laboratory scale Sequencing Batch Reactor (SBR) fed with supernatant of anaerobic digestion of sewage sludge. This effluent was concentrated progressively in order to enhance biomass capacity without inhibiting it. During the first part of the study, ammonium nitrogen was converted to nitrate, so conventional nitrification took place. When a nitrogen ...
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Continuous flow aerobic granular sludge reactor for dairy wastewater treatment
The focus of this study was to assess the treatment performance and granule progression over time within a continuous flow reactor. A continuous flow airlift reactor was seeded with aerobic granules from a laboratory scale sequencing batch reactor (SBR) and fed with dairy wastewater. Stereomicroscopic investigations showed that the granules maintained their integrity during the experimental ...
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Rapid quantification of polyhydroxyalkanoates (PHA) concentration in activated sludge with the fluorescent dye Nile blue A
The present study was conducted (1) to develop a rapid quantification method of polyhydroxyalkanoates (PHA) concentration in activated sludge by Nile blue A staining and fluorescence measurement and (2) to perform on-line monitoring of PHA concentrations in activated sludge. Activated sludge samples collected from laboratory scale sequencing batch reactors and full-scale wastewater treatment ...
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Exploration of rapid start-up of the CANON process from activated sludge inoculum in a sequencing biofilm batch reactor (SBBR)
In this study, a laboratory-scale sequencing biofilm batch reactor (SBBR) was employed to explore a fast start-up of completely autotrophic nitrogen removal over nitrite (CANON) process. Partial nitrification was achieved by controlling free ammonia concentration and operating at above 30 °C; then the reactor was immediately operated with alternating periods of aerobiosis and anaerobiosis to ...
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Influence of seasonal temperature fluctuations on two different partial nitritation-anammox reactors treating mainstream municipal wastewater
Partial nitritation-anammox (PN-A) has gained increasing interest for municipal wastewater treatment in recent years due to its high energy-saving potential. Moving the PN-A technology from side- to mainstream exhibited a set of challenges. Conditions are quite different, with much lower ammonium concentrations and temperatures. Biomass retention becomes highly important due to the even lower ...
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Co-treatment of landfill leachate in laboratory-scale sequencing batch reactors: analysis of system performance and biomass activity by means of respirometric techniques
Aged or mature leachate, produced by old landfills, can be very refractory; for this reason mature leachate is difficult to treat alone, but it can be co-treated with sewage or domestic wastewater. The aim of the study was to investigate the feasibility of leachate co-treatment with synthetic wastewater, in terms of process performance and biomass activity, by means of respirometric techniques. ...
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Laboratory-scale anaerobic sequencing batch reactor for treatment of stillage from fruit distillation
This work describes batch anaerobic digestion tests carried out on stillages, the residue of the distillation process on fruit, in order to contribute to the setting of design parameters for a planned plant. The experimental apparatus was characterized by three reactors, each with a useful volume of 5 L. The different phases of the work carried out were: determining the basic components of the ...
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Role of ammonia-oxidizing bacteria in micropollutant removal from wastewater with aerobic granular sludge
Nitrifying wastewater treatment plants (WWTPs) are more efficient than non-nitrifying WWTPs to remove several micropollutants such as pharmaceuticals and pesticides. This may be related to the activity of nitrifying organisms, such as ammonia-oxidizing bacteria (AOBs), which could possibly co-metabolically oxidize micropollutants with their ammonia monooxygenase (AMO). The role of AOBs in ...
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Nutrient removal, microbial community and sludge settlement in anaerobic/aerobic sequencing batch reactors without enhanced biological phosphorus removal
Nutrient removal, microbial community and sludge settlement were examined in two 3-litre laboratory-scale anaerobic/aerobic sequencing batch reactors (SBRs). One SBR was operated at 10°C and the other SBR at 20°C. Different from conventional enhanced biological phosphorus removal, most of the soluble sodium acetate was removed in the aerobic phase and no organic carbon uptake or biological ...
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Long-term operation of a reactor enriched in
Accumulibacter clade I DPAOs: performance with nitrate, nitrite and oxygenThe microbiology of denitrifying enhanced biological phosphorus removal systems has been a subject of much debate. The question has centred on the affinities of different types of Candidatus Accumulibacter PAOs, type I and type II, towards different electron acceptors such as oxygen, nitrate and nitrite. This study used a propionate anaerobic/anoxic/aerobic lab-scale sequencing batch reactor ...
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A distillery by-product as an external carbon source for enhancing denitrification in mainstream and sidestream treatment processes
The use of fusel oil as an ‘alternative’ carbon source for denitrification in the mainstream and sidestream treatment processes was studied. Research comprised two kinds of batch experiments as well as acclimation of process biomass to external carbon sources. In the conventional nitrate utilization rate (NUR) measurements (one-phase experiments with non-acclimated biomass), the NUR with fusel ...
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Formation of granules with filamentous microorganisms abundant in anaerobic sequencing batch reactor
The start-up and granulation in a lab-scale Anaerobic Sequencing Batch Reactor (ASBR) fed with synthetic glucose wastewater were investigated. Sludge granulation proceeded after 110 days operation and was completed in approximately 140 days. Scanning electron microscope images showed that filamentous bacteria like Methanosaeta are abundant in the granule and exhibit in a uniform structure. The ...
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