Pseudomonas Articles
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Decolorization of industrial azo dye in an anoxic reactor by PUF immobilized
Pseudomonas oleovorans This work reported the full degradation of an azo dye in a synthetic effluent by Pseudomonas oleovorans immobilized in polyurethane foam (PUF). For each fed-batch experiment, a screw-top vessel containing 160 mL of nutrient broth was inoculated with 0.16 g L−1 of fresh culture, incubated at 32 °C and supplemented with 50 mg L−1 of dye every 24 hours. Afterwards, the P. oleovorans were immobilized ...
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Optimisation of process variables for microbial degradation of phenol by binary mixed culture of Pseudomonas aeruginosa and Pseudomonas fluorescens using response surface methodology
The removal efficiency of phenol from aqueous solutions was measured using an indigenous freely suspended binary mixed culture of Pseudomonas aeruginosa and Pseudomonas fluorescens. Experiments were performed as a function of temperature (25?45°C), aeration (1.0?3.5 vvm) and agitation (200?600 rpm). Optimisation of these three process parameters for phenol biodegradation was studied. ...
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Estimation of the energetic parameters associated with the continuous aerobic biodegradation of phenol by monoculture of Pseudomonas aeruginosa and Pseudomonas fluorescence
The objective of this study was to estimate and analyse the growth parameters of indigenous monoculture of Pseudomonas aeruginosa and Pseudomonas fluorescence on phenol in an aerobic chemostat culture. The biodegradation data were checked for consistency using carbon and available electron balances and growth parameters were estimated using two forms of growth models: reparameterised Pirt's model ...
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Biosorption of copper(II) by Pseudomonas aeruginosa
The mechanism of accumulation of copper(II) by Pseudomonas aeruginosa was investigated. Uptake consisted of a rapid process (likely to be extracellular binding) followed by a slow phase (possibly cellular uptake). The sorption capacity of the microbe was found to be 50 mg/g, and sorption followed the Langmuir isotherm. The presence of mild mineral acids (0.1 N HCl) led to destructive ...
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Immobilised microbial reactor for heavy metal pollution control
The copper removal potential of Pseudomonas aeruginosa immobilised on different adsorbent resins has been studied. Duolite S-761 was found to be the most suitable matrix for bacterial immobilisation. Column studies were conducted using virgin as well as bacteria-immobilised resin. The experimental results reveal that resin with immobilised cells as sorbent increased the column capacity by more ...
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Phenanthrene removal from liquid medium with emphasis on production of biosurfactant
In the current study, there are some considerable issues which focused on: (1) the production of biosurfactant, (2) its correlation with the initial bacterial inoculum in the liquid phase and (3) its effect on polycyclic hydrocarbon (PAH) bioremediation performance. Therefore, two strains Pseudomonas facilis and Pseudomonas spp. were able to form a large clear zone diameter on the oil ...
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Nitrate removal by nitrate-dependent Fe(II) oxidation in an upflow denitrifying biofilm reactor
A continuous upflow biofilm reactor packed with ceramsite was constructed for nitrate removal under an anaerobic atmosphere without an organic carbon source. Denitrifying bacteria, Pseudomonas sp. W1, Pseudomonas sp. W2 and Microbacterium sp. W5, were added to the bioreactor as inocula. Nitrate concentration, nitrite accumulation and nitrogen removal efficiency in the effluent were investigated ...
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Type ii hydride transferases from different microorganisms yield nitrite and diarylamines from polynitroaromatic compounds
Homogenous preparations of XenB of Pseudomonas putida, pentaerythritol tetranitrate reductase of Enterobacter cloacae, and N-ethylmaleimide reductase of Escherichia coli, all type II hydride transferases of the Old Yellow Enzyme family of flavoproteins, are shown to reduce the polynitroaromatic compound 2,4,6-trinitrotoluene (TNT). The reduction of this compound yields ...
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Effect of silver-doped phosphate-based glasses on bacterial biofilm growth
Silver-containing phosphate-based glasses were found to reduce the growth of Pseudomonas aeruginosa and Staphylococcus aureus biofilms, which are leading causes of nosocomial infections. The rates of glass degradation (1.27 to 1.41 µg·mm–2·h–1) and the corresponding silver release were found to account for the variation in biofilm growth inhibitory ...
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An assessment of environmental conditions for control of downy brome by Pseudomonas fluorescens D7
Purpose: We evaluated the conditions that favoured Pseudomonas fluorescens strain D7 (P.f. D7) growth and inhibition of downy brome. Design/methodology/approach: Tn5 mutagenesis and a competitive assay were used to isolate mutants of P.f. D7. Isolates were screened for polysaccharide production and toxin response. Seven mutants were tested under varying pH, temperature and water potential and ...
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Biosorption of Pb(II) from synthetic wastewater onto Pseudomonas aeruginosa
The biosorption property onto Pseudomonas aeruginosa was represented by the Langmuir isotherm, and the maximum capacity of Pb(II) was 45 mg g−1 dry weight of bacteria at pH 5. The biosorption capacity of Pb(II) in a binary system increased with increasing Pb(II) concentration, from 0 to 50 mg l−1 in which Cr(III) existed at a constant concentration of 50 mg l−1. This result indicates that there ...
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Energetic analysis of Pseudomonas fluorescens growth in aerobic chemostat culture
Bioenergetic analysis of the growth of Pseudomonas fluorescens on phenol chemostat culture was carried out. The data were checked for consistency using carbon and available electron balances. Similar estimates were obtained using Pirt's model based on Monod approach and a modified model based on substrate consumption rate being rate limiting. Coupled with the covariate adjustment estimation ...
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N -methyl-2-pyrrolidone-degrading bacteria from activated sludgeN-methyl-2-pyrrolidone (NMP) is a widely used solvent for many organic compounds and a component found in a vast array of chemical preparations. For this research paper, NMP degrading bacteria were isolated from two samples of activated sludge. They pertained to both Gram-negative and Gram-positive members, and belong to the Pseudomonas, Paracoccus, Acinetobacter and Rhodococcus genera. All the ...
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Reduction of start-up time through bioaugmentation process in microbial fuel cells using an isolate from dark fermentative spent media fed anode
An electrochemically active bacteria Pseudomonas aeruginosa IIT BT SS1 was isolated from a dark fermentative spent media fed anode, and a bioaugmentation technique using the isolated strain was used to improve the start-up time of a microbial fuel cell (MFC). Higher volumetric current density and lower start-up time were observed with the augmented system MFC-PM (13.7 A/m3) when compared with ...
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Response surface methodology for cadmium biosorption on Pseudomonas aeruginosa
In this research the effects of various physicochemical factors on Cd2+ biosorption such as initial metal concentration, pH and contact exposure time were studied. This study has shown a Cd2+ biosorption, equilibrium time of about 5 min for Pseudomonas aeruginosa and the adsorption equilibrium data were well described by Langmuir equation. The maximum capacity for biosorption has been ...
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The stress response of Pseudomonas species to silver nanoparticles at molecular level
In recent years silver nanoparticles (AgNPs) have been shown to possess broad antibacterial activity. The present study investigated the cytotoxicity of AgNPs on a common soil bacterium Pseudomonas sp. Further the molecular mechanism involved in its stress response to AgNPs was studied. The minimum inhibitory concentration (MIC) of AgNPs was found to be 0.2 mg/L. At sublethal concentration of ...
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Consumption of edible ice contaminated with Acinetobacter, Pseudomonas, and Stenotrophomonas is a risk factor for fecal colonization with extended-spectrum β-lactamase-producing Escherichia coli in Vietnam
Although Vietnamese residents frequently harbor extended-spectrum β-lactamase-producing Escherichia coli (ESBL-E), it is unclear which foods/beverages are risk factors for acquiring these bacteria. The aim of this study was to evaluate the frequency with which edible ice served in restaurants is contaminated with antibiotic-resistant bacteria, and thereby clarify whether this product poses a ...
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Isolation and characterization of some polycyclic aromatic hydrocarbon degrading bacteria from Nsukka soils in Nigeria
Abstract: Twenty-four bacteria capable of utilizing naphthalene, as their sole source of carbon and energy for growth were isolated from three different sites in Nsukka, Nigeria. By standard bacteriological methods, these bacteria were characterized taxonomically as belonging to the genus Pseudomonas, Burkholderia or Actinomycetes. Two of the isolates, which showed the highest growth during ...
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Phenotypic heterogeneity of Pseudomonas aeruginosa isolates in the protected nature park ‘Palić’ (Serbia)
Pseudomonas aeruginosa is a globally distributed environmental bacterium, which is also a significant opportunistic pathogen of humans, animals and plants. It is considered that wide distribution of this bacterium is connected with its most significant constitutive property to form biofilms, and that this multicellular mode of growth, predominant in nature, serves as a protective ...
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Vermont Dry Cleaner Remediation Using CL-Out
Cl-Out bioremediation was used to remove PCE from ground water at a dry cleaners in Vermont. CL-Out micorbes were selected for bioremediation of the site because they are compatible with the natural aerobic aquifer conditions. One application of CL-Out bioaugmentation reduced the PCE concentration by 90% in less than 6 months without increasing the vinyl chloride concentrations. The following ...
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