RNA detection Articles
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Rna silencing gene truncation in the filamentous fungus aspergillus nidulans
The genus Aspergillus is ideally suited for the investigation of RNA silencing evolution because it includes species that have experienced a variety of RNA silencing gene changes. Our work on this subject begins here with the model species Aspergillus nidulans. Filamentous ascomycete fungi generally each encode two of the core RNA silencing proteins, Dicer and Argonaute, but A. nidulans appears ...
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Development of a real-time RT-PCR assay combined with ethidium monoazide treatment for RNA viruses and its application to detect viral RNA after heat exposure
A method was developed for discriminating damaged viruses or naked viral RNA from intact viruses by ethidium monoazide (EMA) treatment before RT-PCR. The applied EMA treatment consisted of three steps: (1) EMA dose, (2) exposure to light, and (3) additional purification by spin-column gel filtration. Approximately 4-log reduction in viral RNA concentration was observed by adding a dose of 10 ...
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Tracking Omicron With Wastewater Data
Testing wastewater for viruses can yield an unbiased epidemiological snapshot of an entire community Since early in the pandemic, wastewater-based epidemiology (WBE), also known as sewershed surveillance, has been used to track COVID-19. It’s a technique that detects chemicals or pathogens in a population by monitoring municipal wastewater, and it has a distinct advantage over individual ...
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Human norovirus infection of Caco-2 cells grown as a three-dimensional tissue structure
Human norovirus (hNoV) infectivity was studied using a three-dimensional model of large intestinal epithelium. Large intestine Caco-2 cells were grown in rotating wall vessel bioreactors for 18–21 days at 37°C and then transferred to 24-well tissue culture plates where they were infected with GI.1 and GII.4 human noroviruses collected from human challenge trials and various outbreak settings, ...
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Discrimination of gastric cancer from normal by serum RNA based on surface-enhanced Raman spectroscopy (SERS) and multivariate analysis
I. INTRODUCTION Currently gastric cancer is the second leading cause of cancerassociated death; accounting for approximately 600 000 annual deaths worldwide.1 The patients with gastric cancer have poor survival rates mainly due to the advanced stages upon the initial time of diagnosis when cancer cells have metastasized into other parts of the body. Early diagnosis and localization of malignant ...
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What is High-Throughput RNA-Sequencing?
High-throughput RNA-sequencing (RNA-seq) is the use of next generation sequencing technology to explore the transcriptome of a genome. It allows researchers to characterize and compare gene expression profiles of biological samples. RNA-seq is now one of the most common tools in molecular biology. In this article, we discover the RNA-seq technique, how it works, and how new RNA-seq ...
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Covid in Wastewater
Sewage and wastewater help in the fight against COVID-19 The World Health Organization declared SARS-CoV-2, known as COVID-19, a worldwide pandemic in March 2020. The world health organization has since reported 35.3 million cases and over 1 million deaths. Despite widespread efforts to social distance, wear masks, and reduce travel, infection rates keep climbing. Infectious disease researchers ...
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How to Improve Your Viral Nucleic Acid Extraction Processes
The extraction of DNA or RNA is a crucial step for the successful detection of viruses. Due to numerous epidemics in recent decades, the demands on diagnostics have increased worldwide. Laboratories are confronted with more and more samples that have to be processed in the same time. To speed up your virus diagnostics, Analytik Jena has developed a new solution for high-throughput extraction ...
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Microbial Detection: A Significant Pillar in Microbiology and Public Health
Microbiology, the study of the smallest and most abundant creatures, is a scientific discipline that transcends the imagination. Among its myriad fields of applications is the subject of microbial detection. This subject holds the utmost importance for public health, food safety, pharmaceuticals, environmental screening, and beyond. This article aims to elucidate on the significant realm of ...
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A new method for efficient detection of Cryptosporidium RNA by real-time reverse transcription-PCR with surfactants
Cryptosporidium is one of the most common causes of waterborne diseases worldwide. Its oocysts possess a robust wall that is extremely resistant to the chlorine used for potable water disinfection. The current procedures of nucleic acid extraction and purification, such as the freeze–thaw (F/T) method and the commercial kits, are time consuming and expensive. To this end, a surfactant ...
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A new approach for detecting adventitious viruses shows Sf-rhabdovirus-negative Sf-RVN cells are suitable for safe biologicals production
Abstract Background Adventitious viral contamination in cell substrates used for biologicals production is a major safety concern. A powerful new approach that can be used to identify adventitious viruses is a combination of bioinformatics tools with massively parallel sequencing technology. Typically, this involves mapping or BLASTN searching individual reads against viral nucleotide ...
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