sample manipulation Articles

  • Dielectrophoresis as a tool for nanoscale DNA manipulation

    The use of the dielectrophoresis as a tool for DNA manipulation is demonstrated experimentally, using both unmodified 48,500 base pairs long bacteriophage lambda dsDNA (λ-DNA), ∼16 μm in length and 414 base pairs long thiol modified natural dsDNA (avDNA), ∼140 nm in length. We show that both the dsDNA types used, are effectively directed between the planar gold electrodes by the positive ...


    By Inderscience Publishers

  • Magnetic nanocarriers: from material design to magnetic manipulation

    Magnetic nanocarriers play an increasing role in various biomedical applications such as the separation of magnetically 'tagged' DNA, drug delivery or identification of biological species. Recent developments in nanotechnology allow the fabrication of both artificial nanocarriers and magnetic separation devices which may achieve great performances at an incredibly small-volume sample handling. ...


    By Inderscience Publishers

  • What is a Spectrometer?

    The characteristics of a white LED light differs greatly, when compared to a yellow CFL. Without any special tools, one can see that the LED emits a clear white color, while the CFL generates yellow light. Going one step further, using a spectrometer, one will be able to “dissect” the light sources and see that they are composed of other colors in the visible light spectrum, such as ...


    By Larson Electronics LLC

  • Case Study: Manipulating Groundwater Redox Conditions for MTBE Remediation

    A pilot study was conducted to investigate the potential to foster anaerobic methyl tert butyl ether (MTBE) biodegradation. The pilot test site, located on Long Island, New York, is characterized as a glacial out-wash aquifer with a high groundwater velocity over 2 ft/day. The MTBE plume is typical of similar plumes that have quickly migrated in this glacial out-wash aquifer. It is ...


    By REGENESIS

  • Sample Handling Strategies for Accurate Lead-In-Soil Measurements in the Field and Laboratory

    ABSTRACT The inhomogenous lead-in-soil matrix can present serious obstacles to accurate sample collection and handling. In typical lead-in-soil measurement, particle size related errors in sampling and sample handling often exceed all other sources of error. The magnitude of error can vary widely depending on the particulate nature of the lead contaminant and the effectiveness of control ...

  • Unraveling a municipal effluent's toxicity to Tripneustes gratilla sperm fertilization

    A tiered investigative approach was employed to characterize and identify substances responsible for the persistent toxicity of a municipal primary‐treated effluent to gametes of the Hawaiian sea urchin, Tripneustes gratilla. EPA Toxicity Identification Evaluation (TIE) procedures were modified to account for the tolerance of T.gratilla gametes to various sample manipulations. Microtox rapid ...


    By John Wiley & Sons, Ltd.

  • Novel carotenoid-based biosensor for simple visual detection of arsenite: characterization and preliminary evaluation for environmental application

    A novel whole-cell arsenite biosensor was developed using the photosynthetic bacterium Rhodopseudomonas palustris no. 7 and characterized. A sensor plasmid containing the operator-promoter region of the ars operon and arsR gene from Escherichia coli and the crtI gene from R. palustris no. 7 was introduced into a blue-green mutant with crtI deleted, R. palustris no. 711. The biosensor changed ...

  • The effects of the ph-influenced structure on the dielectric properties of Kaolinite–water mixtures

    The properties of clay–water mixtures are reflected in their wide-frequency dielectric responses that result from different polarization mechanisms. This feature is useful in various soil science applications but has not been systematically investigated. The objective of this study was to characterize the broadband dielectric spectrum of kaolinite sediments with different charge properties and ...

  • Software review: dips v.5.0

    Dips (5.0) for WINDOWS is the latest version of the brawny steregraphic projection program from Roescience'. It comes packed with enoough functionality to provide solutions to the most complex problems in the fields of Earth Sciences or Engineering requiring the analysis of orientation based data. Dip has obviou applications in the fields of: Stability/instability investigations in mining or ...


    By Rocscience Inc.

  • Ratios of microbial biomass estimates to evaluate microbial physiology in soil

    Abstract  Soil microbial biomass data derived from fumigation–extraction (FE), substrate-induced respiration (SIR) and ATP estimations differed significantly and were significantly correlated, which agrees to previous studies. In a second step, the SIR/FE, ATP/FE and SIR/ATP ratios were calculated to evaluate the glucose-responsive and active component of the microbial (active and resting) ...


  • Projected leaf area and flow rate - options and observed effects on gas exchange measurements with CIRAS-2

    From a practical and theoretical standpoint, the objective in using gas exchange technology is to capture representative plant/soil physiological data, holding instrument error to a minimum. A basic goal in this is to ensure that sampling reflects real responses to natural or imposed environmental variability (treatments). Whether from an observational or experimental approach, the physiological ...


    By PP Systems

  • Site selection of municipal solid waste landfills using analytical hierarchy process method in a geographical information technology environment in giroft

    As an outstanding sample of being contaminated with different kinds of anthropogenic manipulations, environment is successively encountered with ever increasing rate of manmade waste generation. Municipal solid waste generation is among the most significant sources that threaten the global environmental health. Accordingly, it is essential that integrated systems of waste management be considered ...


    By University of Tehran

  • A molecular‐based approach for examining responses of eukaryotes in microcosms to contaminant‐spiked estuarine sediments

    Ecotoxicological information for most contaminants is limited to a small number of taxa, and these are generally restricted to comparatively hardy organisms that are readily extractable from test media and easily identifiable. Advances in DNA sequencing can now provide a comprehensive view of benthic invertebrate diversity. The authors applied 454 pyrosequencing to examine the responses of ...


    By John Wiley & Sons, Ltd.

  • QMax - stand-alone quantification analysis application based on a weighted multi-band CLS Algorithm

    What does QMax do? Allows the user to create an SPGAS Quantification set based on pre-collected or generated standard calibrated spectra. Quantification analysis based on the information provided by the quantification set. On screen display of the quantification analysis, which includes: concentration estimation, standard error of the estimation, and residual ...


    By CIC Photonics, Inc.

  • Biotreatment of Odors from the Zimpro Sludge Conditioning Process

    Management of odor emissions generated during thermal sludge conditioning is a well known disadvantage of the “Zimpro” process. Traditional methods of using chemical oxidation and high temperature destruction result in the formation of secondary products, such as chlorine gas, organic halocarbons or nitrogen oxides. In this study, biotrickling filter technology was evaluated at the Villa Hills ...


    By PRD Tech, Inc.

  • Toxicity Identification Evaluation (TIE) as a Tool for Water Quality Management

    Whole effluent toxicity (WET) testing has evolved into a critical element of many National Pollutant Discharge Elimination System (NPDES) permits issued to both industrial and municipal dischargers. Over 6,500 dischargers to both fresh and salt waters are required to conduct toxicity tests to determine if their effluent might be potentially toxic to organisms in the receiving system. While ...


    By AECOM

  • Mint Miner Case Study: Quality Assurance Solutions, LLC

    Quality Assurance Solutions, LLC (QAS), has been using Mint Miner to review environmental testing data since 2003. “I believe strongly in this product and give out demo copies to every client I meet,” says Diane Lawver, President of QAS. “The product is well-designed and focused on the issues around assuring the integrity of organics data used for decision-making.” QAS uses Mint Miner to ...


    By Promium LLC

  • On-Line Nitrate/Nitrite Probes for BNR Process Control

    ABSTRACTReal time nitrogen monitoring plays an increasingly important role for the successful biological nutrient removal at wastewater treatment plants. Though traditionally dissolved oxygen has been the main process control parameter a greater understanding of the biological mechanisms and process conditions like nitrite/nitrate concentrations to achieve lower effluent values becomes necessary, ...

  • Case Study: Wheeling Water Department, West Virginia

    Wheeling Water Department in Wheeling, West Virginia, is an independent, municipally owned water utility, serving a population of approximately 50,000 people, including all of Ohio County. Consisting of three divisions, Wheeling Water is responsible for all treatment and testing of the source, finished water and for pumping it to the customers. The utility treats approximately 7.5 million gallons ...


    By AllMax Software, Inc.

  • Coagulation, flocculation and agglomeration

    Agglomeration Agglomeration refers to the formation of more or less firmly bound primary particles by collision processes between primary particles, primary particles and agglomerates, as well as pre-existing agglomerates. The agglomeration consists of a destabilization and a transport process. The destabilization is divided into coagulation and flocculation. In general, both ...

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