tissue sample preparation Articles

  • Best practices in mouse tissue sample preparation for RNA extraction with Precellys® evolution

    The lab mouse is the most commonly used mammalian model system for genetic research. Scientists from a wide range of biomedical field shave used the mouse because of its close genetic and physiological similarities to humans, as well as the ease with which its genome can be manipulated and analyzed.Sample preparation is a critical step before proceeding to molecular down-stream analysis. ...


    By Bertin Instruments SAS

  • Analysis of biogenic amines food and physiological samples

    Histamine and other polyamines such as Putrescine, Cadaverine, Spermidine and Spermine are products of decomposition. The level of histamine, in particular, has been used as a regulatory guideline for degree of decomposition in fish. Also, Histamine and Tyramine levels are of interest to producers of wine and cheese. The polyamines, Spermidine, Spermine and Putrescine are elevated in neoplastic ...

  • Recycling Solvents in Histology Department of Hospitals

    Industrial rotary evaporator: eco-friendly and cost-saving Solvents (ethanol, xylene and water) are used in histology for dewatering and destaining during the preparation of tissue sections. Here we show how these solvents are recycled in an automated process using an industrial rotary evaporator. We offer safe and efficient industrial distillation by intuitive ...


    By BÜCHI Labortechnik AG

  • Development and comparison of four methods for the extraction of antibiotics from a vegetative matrix

    Studies have shown the potential for antibiotic uptake into food crops from irrigation water and soils containing pharmaceuticals. The objective of this study was to develop and compare methods quantifying uptake of antibiotics in food crops. Four methods were evaluated utilizing freeze and thaw cell lysing (FT), mechanical maceration (MAC), tissue sonication (SON), and microwave assisted ...


    By John Wiley & Sons, Ltd.

  • Prevalence of Community-Associated Methicillin-Resistant Staphylococcus Aureus in High School Wrestling Environments

    Methicillin-resistant Staphylococcus Aureus (MRSA) was predominantly a hospital-acquired organism; recently, however, community-associated MRSA (CA-MRSA) has been causing outbreaks in otherwise healthy individuals involved in athletics. As such, CA-MRSA is of emerging concern to sanitarians and public health officials. Secondary school athletic trainers and student athletes may be at elevated ...

  • Analysis of Na, K, Ca and Mg in Mineralwater using the Microsampling Method

    Sodium, Potassium, Calcium, and Magnesium belong to the essential mineral substances in the human organism. These elements take influence in the generation of enzymes and hormones, control the osmotic pressure in tissues and body fluids and are important for the exchange procedures in the cell membranes. In this application, the control of Na, K, Ca and Mg in a variety of mineral waters has been ...

  • Single-Cell RNA Sequencing in a fast and inexpensive format

    Sterlitech Corporation’s unique track-etch polycarbonate membrane filters are helping MIT Researchers pioneer a faster and less expensive means of identifying key genes turned on in response to infection or diseases, as featured in the February 13 issue of Nature Methods from the Shalek and Love Labs at ...


    By Sterlitech Corporation

  • Microflow LC offers better sensitivity due to reduced matrix effects!

    What is matrix effect? In my last post, What is Microflow,  I talked about two main parameters,  better sampling efficiency and reduced matrix effect, for microflow LC/MS offering higher sensitivity than its conventional counterpart. Let’s take a close look at the matrix effect. It is a result of ...


    By Waters Corporation

  • Surface Decontamination of Organic Acids (Benzoic Acid) by DeconGel 1101

    Abstract Surface decontamination efficacy determination of DeconGel™ 1101 on stainless steel, aluminum, and concrete surfaces contaminated with Organic Acids (benzoic acid (BA)) was performed with LC/MS (Liquid Chromatography/Mass Spectrometry) according to Environmental Protection Agency (EPA) SW-846 Methods: 3500C (sampling) and 8321B (analysis). Hazardous ...


    By KT Chemicals, Inc.

  • How can I measure Salt in food?

    Sodium is a mineral required by the body for normal functions, but excessive intake can cause high blood pressure and hypertension. This has lead governments and regulatory bodies to focus on monitoring the levels of salt in foods in order to reduce the risk of these health affects. Canned foods are of particular concern as these often contain relatively high levels of salt. ...


    By Camlab Limited

  • o-Phthalaldehyde Reagent

    Introduction Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan under basic conditions. At a pH>9 and ambient temperature, reaction is generally complete within 30 seconds. The products of this reaction, 1-alkyl-2-alkylthio-substituted isoindoles, exhibit optimal excitation at 330 nm and maximal emission at 465 nm. The reagents ...

  • Legal high, cocaine and ketamine application note | Spectroscopy Solutions

    Our Education Business Partnership collaboration with Mia Abbott and Staffordshire University has yielded a new application note on the confirmatory testing of the legal high mephedrone along with illegal drugs ketamine and cocaine and other cutting agents. Read the application note to see the actual results ...


    By Specac Limited

  • BUCHI NIR is Pro-Food Quality at ProFood Tech

    The BUCHI wagon got put back on the road for the ProFood Techconference in Chicago this week. Hopefully you’ll catch us at our booth at Lakeside Upper Hall #3113 (vs. catching our booth attendants just lurking the show floor devouring free samples all day). ProFood Tech is an event, ...


    By BÜCHI Labortechnik AG

  • The convenient and reliable alternative to chemical-based gas measurement within your incubators - Case Study

    With a typical laboratory having many incubators of different types and ages, checking CO2 and O2 levels is a challenge.  Even the most modern incubators need to be checked.  An electronic gas analyser offers a faster and more convenient method than historically popular chemical analysis.  Geotech looks at why their digital gas analyser, the G100, is fast becoming the go to product ...

  • Asbestos in cigarettes

    The original version of the Kent Micronite cigarette filter used crocidolite, a form of asbestos, from 1952 until at least mid-1956. Cigarettes from intact, unopened packs of the brand from this period were examined. One filter contained approximately 10 mg of crocidolite. Crocidolite structures were found in the mainstream smoke from the first two puffs of each cigarette smoked. At the observed ...


    By Queensland Laboratory

  • Some notes about Fats and Oils in Food and about FAME and FAMEWAX

    In Europe, the last transition periods named in REGULATION (EU) No 1169/2011 on the provision of food information to consumers are running out on Dec. 13th, 2016. Since then, a nutrition declaration is mandatory for nearly all packed food in Europe, wherein the amounts of fat, saturates, carbohydrate, sugars, protein and salt have to be included. The content of the mandatory nutrition ...


    By Restek Corporation

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