uhplc Articles

  • Tips to maintain your HPLC & UHPLC systems and columns

    Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your ...


    By Waters Corporation

  • What can I use to filter my mobile phase?

    If you are using mobile phase that contains salts or, generally, any components that are weighed out for its preparation, you will need to filter the solution before using with HPLC or UHPLC. The most common type of filtration is membrane microfiltration. We sell an extensive group of products for this and they are discussed very well in a blog ...


    By Restek Corporation

  • Increased throughput liquid chromatography and reduced solvent consumption for the determination of isoflavones by UHPLC

    All plant foods are complex mixtures of chemicals including both nutrients and biologically active non-nutrients, referred to as phytochemicals. Soy is known for having high concentrations of several physiologically-active phytochemicals, including isoflavones, phytate (inositol hexaphosphate), saponins, phytosterols and protease inhibitors. The isoflavones are what makes soy unique. Soy ...


    By PerkinElmer, Inc.

  • Quantitative LC-MS/MS Analysis of Microcystin Cyclopeptides: EPA Method 544

    Accurate and sensitive characterization and quantification of a structurally diverse class of microcystin cyclopeptides was accomplished using UHPLC-MS-MS. In this analysis, microcystins-RR, YR, and LR were measured using a Nexera UHPLC with a LCMS-8050 triple quadrupole MS detector with ESI ionization in accordance with EPA Method 544. The results of this study ...

  • High Throughput LC-MS-MS Analysis of Carbendazim in Orange Juice

    Highly sensitive analytical methods are necessary to prevent contaminated produce from entering the food supply. In order to help move fresh imports through customs without delay, methods must be rapid and cost effective, in order to test imports thoroughly. LC-MS offers the highly sensitive, robust measurement needed, while ultra high performance liquid chromatography enables high speed ...

  • What is the pressure limit for my LC column?

    This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ...


    By Restek Corporation

  • Out with the old, in with the new…

    We’re all familiar with the adage, “Out with the old, in with the new,” but we probably tend to think of it as it applies to clothes for donation or past relationships, and maybe not in terms of updating our old laboratory equipment. After attending my first WCBP conference, held in Washington, D.C. during January, and hearing life cycle management and ICH Q12 being discussed ...


    By Waters Corporation

  • ACQUITY Arc System: Transferring Validated HPLC Methods within USP Guidelines

    The thought of transferring established high performance liquid chromatography (HPLC) methods onto a another vendor’s LC, or a new platform like UHPLC/UPLC, may cause a bit of anxiety for those that may be unaware of how easy it can be. The reason that the topic of analytical method transfer induces so much angst is that for the transfer of an LC method to succeed, the method has to work as ...


    By Waters Corporation

  • USLC columns put the right tools in your LC method development toolbox

    Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection The most significant influence on chromatographic peak separation, or resolution, is column selectivity. Unfortunately, column selectivity is also the least understood and most underutilized parameter. To improve selectivity, method developers often concentrate on ...


    By Restek Corporation

  • Binary vs. Quaternary Pumps: High Pressure Mixing vs. Low Pressure Mixing

    Welcome to the next installment in my series on binary vs. quaternary pumps for liquid chromatography! In my previous post, I discussed how gradients are created as well as the importance of having accuracy and precision in your gradient. ...


    By Waters Corporation

  • Binary vs Quaternary Pumps

    How is a Gradient Created? Any liquid chromatograph is an amalgamation of a certain set of components that delivers to the scientist a monograph of the sample they are analyzing.  Traditionally, an LC system consists of a pump, an injector, a column heater, a detector and even more commonly these days a mass spectrometer or mass detector.  In ...


    By Waters Corporation

  • Binary vs. Quaternary Gradients: Precision vs. Accuracy

    In the last blog post I talked about how a gradient is formed by the two different pumps.  Today, we will look at the ability for those pumps to deliver the actual gradient that a scientist requests as well as how precise that delivery is. Before we do that, let’s take a step back and remind everyone the difference ...


    By Waters Corporation

  • High-throughput Microscale Bioanalysis is not a Paradox

    Question: When someone says microscale chromatography, what comes to mind? Increased sensitivity? Reduced sample volume? Enhanced MS sampling efficiency? Yes, yes, and yes! You might also say, “finicky,” “slow,” or “only one person in our group knows how to use it!” Maybe even, “there’s no way I can successfully transfer that method, so why ...


    By Waters Corporation

  • The Advantages of a Targeted 2D LC Approach

    Webinar Highlights | Focus on the Relevant: The Advantages of a Targeted 2D Chromatography Approach Hi folks, I’m Daniel Root, Senior Product Marketing Manager for Multidimensional LC Systems at Waters. I recently conducted a webinar that addressed multidimensional liquid chromatography – an exceptional tool for the improvement of characterization and separation of ...


    By Waters Corporation

  • Binary vs. Quaternary: Which Pump is Right for You?

    How to decide which chromatography system pump is right for you Well, you’ve made it this far and I am guessing that you are coming back because I have provided some useful information to help you understand the differences and nuances of a binary versus ...


    By Waters Corporation

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