uhplc column Articles

  • Tips to maintain your HPLC & UHPLC systems and columns

    Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your ...


    By Waters Corporation

  • What is the pressure limit for my LC column?

    This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ...


    By Restek Corporation

  • USLC columns put the right tools in your LC method development toolbox

    Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection The most significant influence on chromatographic peak separation, or resolution, is column selectivity. Unfortunately, column selectivity is also the least understood and most underutilized parameter. To improve selectivity, method developers often concentrate on ...


    By Restek Corporation

  • Top 16 for 2016 - Restek LC article makes the cut

    If you are an avid chromatographer you likely subscribe to LC/GC magazine or its popular electronic version, E-Separation Solutions. It is definitely the place to go for news and information related to all things Chromatography. Over the years Restek has had many an article published in LC/GC and, full disclosure, we advertise there as well. Who in the business doesn’t? It is the place ...


    By Restek Corporation

  • ACQUITY Arc System: Transferring Validated HPLC Methods within USP Guidelines

    The thought of transferring established high performance liquid chromatography (HPLC) methods onto a another vendor’s LC, or a new platform like UHPLC/UPLC, may cause a bit of anxiety for those that may be unaware of how easy it can be. The reason that the topic of analytical method transfer induces so much angst is that for the transfer of an LC method to succeed, the method has to work as ...


    By Waters Corporation

  • The Clog Blog

    Clogging and over pressuring are definitely problematic for Liquid Chromatography columns. Back in 2005 John Dolan’s sage advice to chromatographers to make an LC column last forever was simple, “Never open the box!” As John pointed out, this is not practical and I have to agree. But as an LC column manufacturer I have seen remarks from customers regarding clogging as though it ...


    By Restek Corporation

  • Binary vs. Quaternary Pumps: High Pressure Mixing vs. Low Pressure Mixing

    Welcome to the next installment in my series on binary vs. quaternary pumps for liquid chromatography! In my previous post, I discussed how gradients are created as well as the importance of having accuracy and precision in your gradient. ...


    By Waters Corporation

  • The Advantages of a Targeted 2D LC Approach

    Webinar Highlights | Focus on the Relevant: The Advantages of a Targeted 2D Chromatography Approach Hi folks, I’m Daniel Root, Senior Product Marketing Manager for Multidimensional LC Systems at Waters. I recently conducted a webinar that addressed multidimensional liquid chromatography – an exceptional tool for the improvement of characterization and separation of ...


    By Waters Corporation

  • Binary vs Quaternary Pumps

    How is a Gradient Created? Any liquid chromatograph is an amalgamation of a certain set of components that delivers to the scientist a monograph of the sample they are analyzing.  Traditionally, an LC system consists of a pump, an injector, a column heater, a detector and even more commonly these days a mass spectrometer or mass detector.  In ...


    By Waters Corporation

  • Changing the Rules with Mass Detection

    How simplified detectors can provide mass data for every analytical run and speed up method development With access to multiple mass spectrometry systems of varying degrees of sophistication, Davy Guillarme (University of Geneva, Switzerland) wasn’t actively looking for new mass detection solutions. Nevertheless, the Waters ACQUITY QDa mass detector caught his eye. Why? Because it opens a ...


    By Waters Corporation

  • High-throughput Microscale Bioanalysis is not a Paradox

    Question: When someone says microscale chromatography, what comes to mind? Increased sensitivity? Reduced sample volume? Enhanced MS sampling efficiency? Yes, yes, and yes! You might also say, “finicky,” “slow,” or “only one person in our group knows how to use it!” Maybe even, “there’s no way I can successfully transfer that method, so why ...


    By Waters Corporation

  • Binary vs. Quaternary: Which Pump is Right for You?

    How to decide which chromatography system pump is right for you Well, you’ve made it this far and I am guessing that you are coming back because I have provided some useful information to help you understand the differences and nuances of a binary versus ...


    By Waters Corporation

  • Webinar Recap: “Developing, Testing, and Troubleshooting Chromatography Method Transfer”

    Hi, I’m Tom Wheat, Principal Scientist at Waters, and I recently hosted a webinar entitled, “Developing, Testing and Troubleshooting Chromatography Method Transfer.” Over the course of the webinar, we discussed the transfer of chromatographic methods that occurs at many stages in the development and production of a product. In its simplest form, the method is moved from one ...


    By Waters Corporation

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