UPLC column Articles

  • Tips to maintain your HPLC & UHPLC systems and columns

    Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your ...


    By Waters Corporation

  • Occurrence and removal efficiency of six polycyclic aromatic hydrocarbons in different wastewater treatment plants

    Occurrence and removal efficiency of six polycyclic aromatic hydrocarbons (PAHs) (fluoranthene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, benzo[ghi]perylene and indeno[1,2,3-cd]pyrene) in wastewater were monitored at different treatment processes in three wastewater treatment plants (WWTPs) by using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). ...


    By IWA Publishing

  • ACQUITY Arc System: Transferring Validated HPLC Methods within USP Guidelines

    The thought of transferring established high performance liquid chromatography (HPLC) methods onto a another vendor’s LC, or a new platform like UHPLC/UPLC, may cause a bit of anxiety for those that may be unaware of how easy it can be. The reason that the topic of analytical method transfer induces so much angst is that for the transfer of an LC method to succeed, the method has to work as ...


    By Waters Corporation

  • High performance liquid chromatography–tandem mass spectrometry for rapid and sensitive analysis of six polycyclic aromatic hydrocarbons in wastewater

    A sensitive and fast method was developed to quantitatively analyse the six polycyclic aromatic hydrocarbons (fluoranthene (FLT), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), benzo[a]pyrene (Bap), benzo[ghi]perylene (BghiP) and indeno[1,2,3-cd]pyrene (INPY)) by high performance liquid chromatography (UPLC) coupling with tandem mass spectrometry (MS/MS). Chromatographic separation was ...


    By IWA Publishing

  • Binary vs. Quaternary Pumps: High Pressure Mixing vs. Low Pressure Mixing

    Welcome to the next installment in my series on binary vs. quaternary pumps for liquid chromatography! In my previous post, I discussed how gradients are created as well as the importance of having accuracy and precision in your gradient. ...


    By Waters Corporation

  • The Advantages of a Targeted 2D LC Approach

    Webinar Highlights | Focus on the Relevant: The Advantages of a Targeted 2D Chromatography Approach Hi folks, I’m Daniel Root, Senior Product Marketing Manager for Multidimensional LC Systems at Waters. I recently conducted a webinar that addressed multidimensional liquid chromatography – an exceptional tool for the improvement of characterization and separation of ...


    By Waters Corporation

  • High-throughput Microscale Bioanalysis is not a Paradox

    Question: When someone says microscale chromatography, what comes to mind? Increased sensitivity? Reduced sample volume? Enhanced MS sampling efficiency? Yes, yes, and yes! You might also say, “finicky,” “slow,” or “only one person in our group knows how to use it!” Maybe even, “there’s no way I can successfully transfer that method, so why ...


    By Waters Corporation

  • Binary vs Quaternary Pumps

    How is a Gradient Created? Any liquid chromatograph is an amalgamation of a certain set of components that delivers to the scientist a monograph of the sample they are analyzing.  Traditionally, an LC system consists of a pump, an injector, a column heater, a detector and even more commonly these days a mass spectrometer or mass detector.  In ...


    By Waters Corporation

  • Is Microflow LC a Reality or Perception?

    Microflow or nanoflow chromatography has been used mostly in the academic and fundamental research environment in the past for its various advantages over analytical liquid chromatography, but rarely in the industrial settings. This is most likely because many of us believe it is a pain-in-the-neck technique to perform! But is this a reality or just a ...


    By Waters Corporation

  • Binary vs. Quaternary: Which Pump is Right for You?

    How to decide which chromatography system pump is right for you Well, you’ve made it this far and I am guessing that you are coming back because I have provided some useful information to help you understand the differences and nuances of a binary versus ...


    By Waters Corporation

  • Webinar Highlights: Microfluidics for macromolecules

    Hi, I’m Jim Murphy, Principal Consulting Chemist at Waters, and I recently collaborated with Lucinda (Cohen) Hittle to present an exciting webinar on the use of microflow chromatography for macromolecules.  Lucinda, a Director of Discovery Bioanalytics at Merck, presented an overview of the use of LC-MS for peptide analysis along with various case studies for in vitro and in vivo ...


    By Waters Corporation

  • Webinar Recap: “Developing, Testing, and Troubleshooting Chromatography Method Transfer”

    Hi, I’m Tom Wheat, Principal Scientist at Waters, and I recently hosted a webinar entitled, “Developing, Testing and Troubleshooting Chromatography Method Transfer.” Over the course of the webinar, we discussed the transfer of chromatographic methods that occurs at many stages in the development and production of a product. In its simplest form, the method is moved from one ...


    By Waters Corporation

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