Microcystins plate statfax 303+ program set-up


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General Comments:
This technical bulletin is a guide to the installation of Microcystins Plate Kit Protocol into the StatFax 303 Plus. Please refer to the StatFax 303 Plus Manual for detailed instructions. Bold lettering indicates the StatFax screen display.

1. Turn on the StatFax 303 Plus.
2. Press the [MULT] key on the keypad.
3. Regression Y/N: Press the [Yes} key.
4. Filter Selection: Press [2] key for 450nm, then [Enter]
5. Diff Filter Selection: Press [0] key for no filter, then [Enter]
6. Offset Abs Y/N: Press the [No] key.
7. Linear Y/N: Press [No] key until Log/Logit Y/N appears. Press [Yes] key.
8. Blank Y/N: Press [No] key.
9. Duplicates Y/N: Press [Yes] key.
10. Dup Calibrtr Y/N: Press [Yes] key.
11. Dup Sample Y/N: Press [Yes] key.
12. Done Y/N: Press [Yes] key.
13. # of Cals: Press [4] key, then [Enter].
14. Select Unit Y/N: Press [Yes] key.
15. Key Unit Code Number: Press [12] for ppb, then [Enter].
16. PPB Y/N: Press [Yes] key.
17. CAL 2: Press [0.1], then [Enter].
18. CAL 3: Press [0.4], then [Enter].
19. CAL 4: Press [1.6], then [Enter].
20. Off Curve Ok Y/N: Press [No] key.
21. Set Carrier (Strip A), Then Press Enter: Place the developed strip in the reader with the 0 ppb negative control facing into the instrument. This should line up with second grove on left. Wells will be read and results printed.
22. Plot Curve Y/N: Press [Yes] key.
23. Accept Curve Y/N: Press [Yes] key.
24. Set Carrier (Strip B), Then Press Enter
25. When all strips are read: Press the [ALT] key to save the protocol.

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