The PicTar program predicted that microRNA-126 (miR-126), miR-145, and let-7s target highly conserved sites within the Hoxa9 homeobox. There are increased nucleotide constraints in the three microRNA seed sites among Hoxa9 genes beyond that required to maintain protein identity, suggesting additional functional conservation. In preliminary experiments, forced expression of these microRNAs in Hoxa9-immortalized bone marrow cells downregulated the HOXA9 protein and caused loss of biological activity. The microRNAs were shown to target their predicted sites within the homeobox. miR-126 and Hoxa9 mRNA are coexpressed in hematopoietic stem cells and downregulated in parallel during progenitor cell differentiation; however, miR-145 is barely detectable in hematopoietic cells, and let-7s are highly expressed in bone marrow progenitors, suggesting that miR-126 may function in normal hematopoietic cells to modulate HOXA9 protein. In support of this hypothesis, expression of miR-126 alone in MLL-ENL-immortalized bone marrow cells decreased endogenous HOXA9 protein, while inhibition of endogenous miR-126 increased expression of HOXA9 in F9 cells.
- American Society for Microbiology
- MicroRNA-126 regulates HOXA9 by binding to the homeobox
MBBR Wastewater Treatment - 5 Top Advantages
Modular wastewater treatment solutions have become very important to treat waste water. Water scarcity has become an important issue worldwide, requiring strategies for both water conservation and effective water and wastewater treatment. Several technologies have been in practice for generations including the conventional activated sludge process.MBBR wastewater treatmentis a relatively new water treatment process being used for filtering waste water in both industrial and domestic sectors with excellent performan...
RCBWG Releases Summary of Best Practices Survey on Engaging Faith Communities
SCB`s Religion and Conservation Biology Working Group (RCBWG) released asummary of the its Best Practices Survey of SCB members on engaging faith communities. Conducted on behalf of RCBWG from May 31-September 10, the results of this survey underscore the benefits to conserving biological diversity when researchers and practitioners relate positively to faith leaders and communities. Respondents to the survey also shared their approaches to engaging leaders and members of faith communities in ways that might be...
Why degraded reefs could be the future of ocean conservation
When there’s not enough pristine habitat left to save an ecosystem, it’s time to take a new look at less-than-perfect places. When you think of a nature preserve, chances are you picture a tract of pristine, healthy wilderness, set aside in order to protect a functioning ecosystem. But what if not much remains of the ecosystem you want to protect? A new proposal by an international group of scientists and conservationists is challenging traditional ideas about what’s worth saving when it comes...
A call to conserve crops’ wild cousins
Wild cousins aren’t always appreciated at family gatherings. But when it comes to crops, the opposite is often true: Plant breeding has historically relied on genes from plants growing in the wild as a source of diversity that can be introduced into crop plants to produce new crop varieties that are more resilient, nutritious and productive than those currently cultivated. As human populations increase and shift away from traditional diets, demand for food is expected to increase dramatically — and...
Action plan for peatland conservation and wise use in Russia. Moscow/ Wetlands International Russia ...
According` the National Land Inventory of 1 January 2000, mires make up 8.2% of the total land resources of the Russian Federation. According to expert evaluation, mires and peat-containing paludified landscapes cover at least 20% of the country`s territory and are a major part of the world`s peatland resource. Peatlands use in the economy is associated with the following problems brought about by the specificity of the resource: A peatland is a complex natural object that affects the water and geochemical...