The mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) plays a major role in dephosphorylating and thereby inactivating the MAP kinases extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. Here, we examine the posttranscriptional events underlying the robust MKP-1 induction by oxidants in HeLa cells. H2O2 treatment potently stabilized the MKP-1 mRNA and increased the association of MKP-1 mRNA with the translation machinery. Four RNA-binding proteins (RNA-BPs) that influence mRNA turnover and/or translation (HuR, NF90, TIAR, and TIA-1) were found to bind to biotinylated transcripts spanning the MKP-1 AU-rich 3' untranslated region. By using ribonucleoprotein immunoprecipitation analysis, we showed that H2O2 treatment increased the association of MKP-1 mRNA with HuR and NF90 and decreased its association with the translational repressors TIAR and TIA-1. HuR or NF90 silencing significantly diminished the H2O2-stimulated MKP-1 mRNA stability; HuR silencing also markedly decreased MKP-1 translation. In turn, lowering MKP-1 expression in HuR-silenced cultures resulted in substantially elevated phosphorylation of JNK and p38 after H2O2 treatment. Collectively, MKP-1 upregulation by oxidative stress is potently influenced by increased mRNA stability and translation, mediated at least in part by the RNA-BPs HuR and NF90.
- American Society for Microbiology
- MKP-1 mRNA stabilization and translational control by ...
Experiences in development and maintenance of Silence-GIS
Abstract Silence-GIS is a larse scale noise management system build as an extension on ArcGIS 9.3.1. This system has been designed and developed for the Dutch Highway Authorities. The aim of the system is to support decisions on Dutch and European noise policy and to predict the effect of future measures on the noise exposure of the population in The Netherlands. The information needed to perform noise calculations was divided over different departments. There was a great need for standardization and integration...
How to manage Silence? A large scale noise management system
AbstractSilence is the main noise management system for the Dutch highways and is used by the Dutch Ministry of Transport. The Silence system comprises IT infrastructure, environmental data, noise management software, an organisation, procedures and maintenance. Typical noise management system tasks involve data management, setup noise management software, software customisation, performing calculations and analyses and a proper maintenance cycle. In 2010 the new noise management system will become available and...
The Silence of the Lambs – Invictus Case Study
The sound of new-born lambs bleating is something that most people would be happy to wake up to on a Spring morning – or perhaps not? Noise consultant Louise Alderson, had the unusual assignment of monitoring noise levels from baby lambs and their mothers on a farm in Cumbria following a planning application to build a housing development on nearby fields – owned by the same farmer. Environmental Health Officers (EHO) from the Local Authority had raised concerns that noise from the annual spring...
Satisfying super-sensitive complainants with psychological silencing
If you play white noise to a group of people and tell them that the song “White Christmas” is hidden in the sound, about 30% of them will be convinced that they can hear it. The human mind is always looking for patterns – even when they aren’t there. This propensity explains a significant proportion of complaints where noise levels are actually very low (they can be close to, or below, the threshold of hearing at low frequencies) or the complainant has mild tinnitus. The imagined pattern...
Record breaking formula one fan noise control
Problem: an extract system fan drone creating a potential environmental noise problem. Solution: aerodynamic engineering instead of silencers – in keeping with the Red Bull Technology ethos. The conventional technique used to reduce fan tones like this is a silencer – a large, palliative silencer that doesn’t tackle the source of the problem. Not only can these reduce the system efficiency, but they may also require additional lagging and regular cleaning to maintain performance. Moreover,...