Keywords: yeast, human insulin yeast producer, RAPD-PCR, DNA finger-printing
Molecular differences and fingerprinting of normal and genetically engineered 'Saccharomyces cerevisiae' human insulin producer
The purpose of the present paper is an attempt to determine the molecular differences and fingerprinting of a normal and genetically engineered Saccharomyces cerevisiae human insulin producer obtained through transformation with human DNA (introns and exons) isolated from healthy human pancreas. Five primers were used; four with 20-mer and one with 10-mer. Gels photographs for all primers using PCR fingerprinting for the original wild-type yeast strain DNA and the human insulin producer yeast strain DNA, showed amplification. The great differences in the bands appeared between the original wild type yeast strain DNA and the human insulin producer yeast strain DNA, indicating that great changes occurred in the structure of DNA in order to offer the capability for insulin production.