Neptune project deliverable D3.1

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Courtesy of IWA Publishing

Ecotoxicity and microbiological testing methods (OECD and DIN/ISO guidelines) optimised for whole effluent testing

Within Workpackage 3 the following tests will be performed:

  • Whole effluent testing (WET) and Toxicity Identification Evaluation (TIE) in fractionated samples for up to 200 samples with tier 1 screening assays: YES (Yeast Estrogenic Screen) and YAS (Yeast Androgenic Screen) for the identification of receptor-mediated (anti-) estrogenic and (anti-) androgenic activities, Yeast Mutagenicity Screen and Cytotoxicity Screen.
  • WET for up to 10 samples with tier 2 tests (chronic and life-cycle exposure), representing key species for the structure and function of aquatic ecosystems in Europe. The following tests will be performed in flow-through design at partner institutes developing the new waste water treatment technologies: (1) Primary producer test (Lemna minor) according to OECD guideline draft, (2) worm (Lumbriculus variegatus) 28-d growth and reproduction test according to OECD guideline draft (3) mollusc (Potamopyrgus antipodarum) 28-d reproduction test according to a new OECD guideline proposal, (4) arthropod (Chironomus riparius) life-cycle test to OECD guideline 218 and (5) Fish Early Life Stage Toxicity (FELST) test with Danio rerio according to an OECD guideline draft.
  • Identification and enumeration of bacterial indicators such as total coliforms, E. coli and enterococci as well as viral indicators such as somatic coliphages according to DIN/ISO guidelines adapted to effluent. The test set will be performed in up to 50 whole effluent samples. Bacterial tests will be performed using membrane filtration (MF) techniques, somatic coliphages using the double-layer agar technique.

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