An experiment was carried out to investigate the performance of mixed ocean bacteria, isolated from the ocean sediment, on landfill leachate treatment. In this treatment, ocean bacteria were the only constituent added to remove organics and NH4+-N. Given their considerable influence on wastewater purification, factors such as inoculum, initial pH, processing time and oxygen condition, were directly involved in this research. As indicated by laboratory test results, chemical oxygen demand (CODCr) and NH4+-N removal could reach 94.45% and 67.87%, respectively, after 3 days of treatment, in conditions of natural pH 6.3 and with the application of oxygen. The volt–ampere characteristics of the bacteria solution verified the redox-active ability of the bacteria in landfill leachate treatment.