Keywords: adsorption capacity, cell culture, cell growth age, cell pretreatment, copper sorption, Pseudomonas putida, biosorption, heavy metals, metal removal, wastewater treatment, water pollution
Optimisation of cell surface and structural components for improving adsorption capacity of Pseudomonas putida 5-x to Cu2+
A heavy metal binding bacterium Pseudomonas putida 5-x was used as biosorbent for heavy metal removal from wastewater. In order to compete with physical adsorbent, the optimisation of the cell preparation technique for further improving its biosorption capacity was conducted. Results showed that the compositions of medium for cell culturing, cell growth age and cell pre-treating technique would affect the cell surface and structural components, hence the adsorption capacity of the cell to heavy metal ions. Using optimal cell culture conditions and pretreatment technique, the Cu2+ adsorption capacity of the cell was obviously increased from 51.2 mg g−1 to 89.6 mg g−1.