John Wiley & Sons, Ltd.

Optimising fluorescent protein choice for transgenic embryonic medaka models

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Early life stage transgenic medaka are recognised as a pertinent model by the OECD and are non‐compliant with the European definition of a laboratory animal. However, auto‐fluorescence confounds readout of fluorescent biomarkers. We determined the fluorescence emission spectrum of different embryonic stages of medaka submitted to a range of excitation wavelengths. This allows selection of high signal to noise ratio fluorescent proteins and combining multiple biomarkers within a single embryo. Environ Toxicol Chem © 2013 SETAC

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