Browse all Channels
IWA Publishing

IWA Publishing
Alliance House, 12 Caxton Street ,
London ,
United Kingdom SW1H 0QS
United Kingdom
  1. Companies
  2. IWA Publishing
  3. Articles
  4. PCR amplification and DNA sequence of mcyA gene: The ...
PCR amplification and DNA sequence of <italic>mcy</italic>A gene: The distribution profile of a toxigenic <italic>Microcystis aeruginosa</italic> in the Hartbeespoort Dam, South Africa

PCR amplification and DNA sequence of mcyA gene: The distribution profile of a toxigenic Microcystis aeruginosa in the Hartbeespoort Dam, South Africa

0
- By: , , , , , ,

Courtesy of IWA Publishing

Using new polymerase chain reaction (PCR) primers, a once known to be under-transcribed microcystin synthetase A (mcyA) gene from the only known toxigenic cyanobacterium Microcystis aeruginosa dominating the Hartbeespoort Dam was consistently amplified from genomic DNA extracted from a set of algal and cell free water samples collected across this dam. In addition to this, five more mcy genes (mcyBCDEG) were also amplified during this study. The resultant mcyA PCR products (518 bp) were purified and sequenced and gave nucleotide sequence segments of 408 bp sizes. The obtained sequence was aligned to the published mcyA gene sequence available online on the NCBI database and resulted in 100% similarity to a 408 bp mcyA gene sequence segment of M. aeruginosa UWOCC RID-1. Furthermore, it was found that the above sequence segment (408 bp) spans from a common base in M. aeruginosa PCC 7806 and M. aeruginosa PCC 7820 from 141 to 548 bp in the N-methyl transferase (NMT) region signifying their closer relatedness to M. aeruginosa UWOCC strains. This study has for the first time amplified mcyA gene consistently from both intracellular and extracellular DNA extracts obtained from algal and cell free water samples, respectively. Sequence data and the amplified mcy genes showed that M. aeruginosa is widely distributed and dominant in this dam.

Related articles

  • Underwater Topography Survey with M40 Autonomous Survey Boat - Case Study

    Location: Jiangmen, South China Date: December 7th, 2017 Mission: A survey task of government department Measuring area: 600m*1,000m Duration: About 5 hours Vessel: M40 Boat Parameters Displacement: 450kg Dimensions: 3.5*1.9*1.6m Maximum speed: 8kn Battery life: 6 hours Payload: 65 kg Communication: 5km Collision avoidance: 50m M40 is a multi-purpose USV compatible with multibeam, single beam, side scan, ADCP, etc. With autonomous navigation function and environmental sensing technology, M40...

  • SL20 USV Conducts Topographic Survey To A River Section - Case Study

    Date: April 16, 2019 Location: A River Section Near Youyi Bridge in Xiangyang City, Wuhan Facility: SL20 Autonomous Survey USV River condition: Relatively strong wind, waves over 10cm high, with ships passing by. Mission: Utilize the SL20 USV to survey the underwater topography and section of this river sector systematically to generate a three-dimensional coordinate system for later mapping processing Survey area The survey area is 150m away from Youyi bridge. In this areathe river is of 200 to 300...

  • ESM30 conducts autonomous water sampling and monitoring - Case Study

    Background Wuhan is the capital city of Hubei province in central China which has 166 lakes that cover 2,217 square km approximately counting 25% of the city. Its water coverage is one of the world’s highest. The Wuhan Environmental Protection Bureau is responsible for monitoring the surface water quality by surveying a subset of 50 lakes of their temperature, conductivity, pH, dissolved oxygen and turbidity on a yearly basis. Before using the Oceanalpha ESM30 water quality sampling & monitoring...

  • ESM30丨USV cooperates with UAV to monitor water quality - Case Study

    ESM30丨USV cooperates with UAV to monitor water quality Time: June 2016 Equipment: ESM 30 USV, UAV Background: Governor Zhu Xiaodan went to Shantou city, Guangdong province to investigate the effectiveness of comprehensive rectification of Lianjiang river. Oceanalpha’s USV, in collaboration with drones of provincial environmental school, provide first-hand water quality data quickly and accurately. Main process: In surveys, drone can take off on USV, fly above river and shoot photos at night....

  • ME40 Conducts Autonomous Bathymetric Survey in Zhenjiang city - Case Study

    Time: April, 2015 Location: Jiangxinzhou, Zhenjiang, Jiangsu Company: Shanghai Waterway Engineering Design and Consulting Co.,Ltd. Project Background: The survey project needs to measure the depth of shallow water area in Yangtze River. The survey area is 50m to the bank and 4km long, in which are full of crushed stones. It is impossible for manned ship to survey in this area. Autonomous boat with low draft is perfect to operate in shallow area. Equipment list ME40 autonomous survey boat Base station Teledy...

Customer comments

No comments were found for PCR amplification and DNA sequence of mcyA gene: The distribution profile of a toxigenic Microcystis aeruginosa in the Hartbeespoort Dam, South Africa. Be the first to comment!