The pour plate method of counting bacteria is more precise than the streak plate method, but, on the average, it will give a lower count.
Triclosan resistant bacteria in sewage effluent and cross-resistance to antibiotics
The purpose of this study was to identify triclosan tolerant heterotrophic plate count (HPC) bacteria from sewage effluent and to determine cross-resistance to antibiotics. R2 agar supplemented with triclosan was utilised to isolate triclosan resistant bacteria and 16S rRNA gene sequencing was conducted to identify the isolates. Minimum inhibitory concentrations (MICs) of organisms were determined at selected concentrations of triclosan and cross-resistance to various antibiotics was performed. High-performance...
Enhanced Bio-Remediation of Wastewater
Bioremediation has been used to treat hydrocarbon contamination in municipal wastewater for centuries. Most microbes used in bioremediation today are naturally occurringyetspecifically chosen and cultured versus being genetically modified. Bioremediation rivals techniques such as pump and treat extraction systems, as it provide cost savings for municipalities, as well as environmental savings by keeping valuable resources active. Technological advances in quality control have allowed specialized bioremediation...
Online waste water monitoring system on golfcourse - Case Study
Validation of BACTcontrol as an online waste water Monitoring System and Microbiological Early Warning System. The presence of dangerous micro organisms in water used in food processing or surface water can be a real threat for human health. The constant monitoring and control of the presence of such dangerous pathogens is therefore of big importance. In a collaboration project between a local Waterboard and microLAN the bacterial activity of the effluent of Kaatsheuvel golf park treatment plant was determined....
Pathogenic features of heterotrophic plate count bacteria from drinking-water boreholes
Evidence suggests that heterotrophic plate count (HPC) bacteria may be hazardous to humans with weakened health. We investigated the pathogenic potential of HPC bacteria from untreated borehole water, consumed by humans, for: their haemolytic properties, the production of extracellular enzymes such as DNase, proteinase, lipase, lecithinase, hyaluronidase and chondroitinase, the effect simulated gastric fluid has on their survival, as well as the bacteria's antibiotic-susceptible profile. HuTu-80 cells acted as...
Bacteriological water quality changes in parallel pilot distribution systems
In a component of an extensive pilot distribution system (PDS) study, the effects of four different water qualities on biological stability in distributed water were investigated through identical (parallel) single-pass pipe arrangements. Through 24 months of monitoring, a number of key observations were made. Incorporation of a biological treatment step reduced the overall dissolved organic carbon (DOC) loss through the PDS by reducing biodegradable DOC (BDOC) within the water prior to distribution. In the...