Procedure for benomyl/carbendazim rapid assay, installation on the RPA-i

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General Comments

New RaPID Assay methods can be entered into the RPA-I memory with either a download cartridge or manually. This Technical Bulletin is a guide to the manual installation of the Benomyl/Carbendazim method on the RPA-I using the keypad. Please refer to the RPA-I Manual for a detailed discussion.

Protocol

1. Turn on the RPA-I.

2. Press the [OPTION] key on the top right side of the keypad.

3. Scroll through the menu by pressing either the [YES] or [NO] keys. Press the [ENTER] key when 'Enter/Edit Protocol' appears.

4. Scroll through the menu using the [YES] or [NO] keys until 'UNUSED [# Avail.]' appears. The '# Avail' indicates how many more protocols the cartridge will accept. Press the [ENTER] key (if 'NO' appears, a protocol must be deleted before a new one can be entered).

5. Protocol Nam: Enter the name of the new protocol 'BEN/CBZM' by scrolling with either the [YES] or [NO] keys until letter 'B' appears. Press the [ENTER] key. Scroll to 'E', press [ENTER]. Continue inputting letters until the entire name is entered. Errors can be corrected by pressing the [BACK SPACE] key.

6. Data Reduct.: Select by scrolling to Lin. Regression and press [ENTER].

7. Xform Option: Select Ln/LgtB and press [ENTER].

8. # of Calibrator: Enter 4 and press [ENTER].

9. Cal. Replicates: Enter 2 and press [ENTER].

10. Cal. #1 Conc: Enter 0.00 and press [ENTER].

11. Cal. #2 Conc: Enter 0.25 and press [ENTER].

12. Cal. #3 Conc: Enter 1 and press [ENTER].

13. Cal. #4 Conc: Enter 5 and press [ENTER].

14. Correlation Flag: Enter 0.990 and press [ENTER].

15. Unit: Enter 'PPB' as in step 5 and press [ENTER] after each letter. Press [ENTER] a second time after the second 'B' to continue.

16. Normal Range Hi: Enter 5 and press [ENTER].

17. Normal Range Low: Enter 0.1 and press [ENTER].

18. # of Controls: Enter 1 and press [ENTER].

19. Ctrl Replicates: Enter 1 and press [ENTER].

20. # of Rgt Blanks: Enter 0 and press [ENTER].

21. Wavelength: Enter 450 and press [ENTER].

22. Read Mode: Scroll to Absorbance and press [ENTER].

23. Rep. %CV Flag: Enter 10 and press [ENTER].

24. List Protocol?: Press the YES key. Check the data entered with the information listed here.R092592

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