Procedure for ensys method installation on the RPA-I

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GENERAL COMMENTS:
Semi-quantitative analysis using the EnSys methods can be programmed by the operator into the RPA-I analyzer. This Technical Bulletin is a guide to thaen mual installation of the EnSys methods on the RPA-I using the keypad. Please refer to the RPA-I Manual for a detailed discussion.

PROTOCOL:
1. Turn on the RPA-I.
2. Press the [OPTION] key on the top right side of the keypad.
3. Scroll through the menu by pressing either the [YES] or [NO] keys. Press the [ENTER] key when 'Enter/Edit Protocol' appears.
4. Scroll through the menu using the [YES] or [NO] keys until 'UNUSED [# Avail.a]p'p ears. The '# Avail' indicates how many more protocols the carirdtge will accept. Press the [ENTER] key (if 'NO' appears, a protocol must be deleted before a new one can be entered).
5. Protocol Nam: Enter the name of the new protocol E'NSYS' by scrolling with either the [YES] or [NO] keys until letter 'E' appears. Press the [ENTER] key. Scroll to ' N', press [ENTER]. Scroll to 'S', press [ENTER]. Continue in this manner to spEeNll SYS Press [ENTER] again to continue. Errors can be corrected by pressing the [BACK SPACE] key.
6. Data Reduct.: Select by scrolling to Qualitative and press [ENTER].
7. Ref. Reps: Enter 1 and press [ENTER].
8. C1=Aref1+Bref2+C: Enter A=1.00 and press [ENTER].
9. C1=Aref1+Bref2+C: Enter B=0.00 and press [ENTER].
10. C1=Aref1+Bref2+C: Enter C=0.00 and press [ENTER].
11. C2=Dref1+Eref2+F: Enter D=0.00 and press [ENTER].
12. C2=Dref1+Eref2+F: Enter E=0.00 and press [ENTER].
13. C2=Dref1+Eref2+F: Enter F=0.00 and press [ENTER].
14. Unkn. <CO1, scroll to Positive and press [ENTER].
15. Unkn. =CO1, scroll to Positive and press [ENTER].
16. Unkn. >CO1, scroll to Negative and press [ENTER].
17. # of Controls: Enter 0 and press [ENTER].
18. # of Rgt Blanks: Enter 0 and press [ENTER].
19. Wavelength: Enter 450 and press [ENTER].
20. Read Mode: Scroll to Absorbance and press [ENTER].
21. Rep. %CV Flag: Enter 30 and press [ENTER].
22. List Protocol?: Press the YES key. Check the data entered with the informatliiosnte d here.

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