Procedure for PAH`s rapid assay installation on the RPA-I


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New RaPID Assay methods can be programmed by the operator into the RPA-I analyzer. This Technical Bulletin is a guide to eth manual installation of the PAH's method on the RPA-I using the keypad. Please refer to the RPA-I Manual for a detailed discussion.

1. Turn on the RPA-I.
2. Press the [OPTION] key on the top right side of the keypad.
3. Scroll through the menu by pressing either the [YES] or [NO] keys. Press the [ENTER] key when 'Enter/Edit Protocol' appears.
4. Scroll through the menu using the [YES] or [NO] keys until 'UNUSED [# Avail.]' appears. The '# Avail' indicates how many more protocols the caidrtgre will accept. Press the [ENTER] key (if 'NO' appears, a protocol must be deleted before a new one can be entered).
5. Protocol Nam: Enter the name of the new protocol 'PAH' by scrolling with either the [YES] or [NO] keys until letter 'P' appears. Press the [ENTER] key. Scroll to 'A', press [ENTER]. Scroll to 'H', press [ENTER]. Press [ENTER] again to continue. Errors can be corrected by pressing the [BACK SPACE] key.
6. Data Reduct.: Select by scrolling toL in. Regression and press [ENTER].
7. Xform Option: Select Ln/LgtB and press [ENTER].
8. # of Calibrator: Enter 4 and press [ENTER].
9. Cal. Replicates: Enter 2 and press [ENTER].
10. Cal. #1 Conc: Enter 0.00 and press [ENTER].
11. Cal. #2 Conc: Enter 2.0 and press [ENTER].
12. Cal. #3 Conc: Enter 10.0 and press [ENTER].
13. Cal. #4 Conc: Enter 50.0 and press [ENTER].
14. Correlation Flag: Enter 0.990 and press [ENTER].
15. Unit: Enter as in step 5 'PPB' and press [ENTER] after each letter. Press [ENTER] a second time after the 'B' to continue.
16. Normal Range Hi: Enter 50 and press [ENTER].
17. Normal Range Low: Enter 0.7 and press [ENTER].
18. # of Controls: Enter 1 and press [ENTER].
19. Ctrl Replicates: Enter 1 and press [ENTER].
20. # of Rgt Blanks: Enter 0 and press [ENTER].
21. Wavelength: Enter 450 and press [ENTER].
22. Read Mode: Scroll to Absorbance and press [ENTER].
23. Rep. %CV Flag: Enter 10 and press [ENTER].
24. List Protocol?: Press the YES key. Check the data entered with the information listed here.

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