John Wiley & Sons, Ltd.

Proteomics analysis of Xenopus laevis gonad tissue following chronic exposure to atrazine

Atrazine is the most commonly detected pesticide contaminant in ground and surface water. Previous studies have showed that atrazine is an endocrine disruptor owing to its adverse effects on the male reproductive system in several vertebrates, but very few molecular mechanisms for these effects have been revealed. In the present study, Xenopus laevis were exposed to 100 ppb atrazine for 120 d, and then isobaric tags for relative and absolute quantitative (iTRAQ) technique was used to detect global changes in protein profiles of the testes and ovaries. Our results showed that 100 ppb atrazine exposure adversely affected the growth of X. laevis, did not induce hermaphroditism but delayed or prevented the development of male seminiferous tubules. Proteomic analysis showed that atrazine altered expression of 143 and 121 proteins in the testes and ovaries respectively, and most of them are involved in cellular and metabolic processes and biological regulation based on their biological processes. In addition, apoptosis, tight junctions, and metabolic pathways were significantly altered in the atrazineā€treated gonads. Based on the above results, we postulate that the reproductive toxicity of atrazine may be due to disruption of tight junctions and metabolic signaling pathways and/or induction of apoptosis in germ cells. This article is protected by copyright. All rights reserved

Customer comments

No comments were found for Proteomics analysis of Xenopus laevis gonad tissue following chronic exposure to atrazine. Be the first to comment!