Ceriporiopsis sp. strain MD-1, isolated from forest soil, produced several extracellular enzymes that decolorized human hair melanin. Among them, three enzymes (E1, E2-1, and E2-2) were purified to homogeneity and characterized. The enzymes required hydrogen peroxide in their enzyme reactions and, typical of other fungal peroxidases, oxidized various phenol compounds such as guaiacol, but not 3,4-dimethoxybenzyl alcohol. The spectra of the three enzymes showed an absorption maximum at 406 nm, indicating that they were heme proteins. However, the A406/A280 values of the enzymes were below 0.4, which was lower than those of other peroxidases. E2-1 and E2-2 were similar to each other in their molecular and catalytic properties, and they possibly represent products of posttranslational modifications and/or allelic variants of the same gene, mdcA. The corresponding cDNA was cloned and sequenced; the deduced amino acid sequence showed high identities to the manganese peroxidases from other microorganisms. The specific activities and Km values of E2-1 and E2-2 for synthetic and human hair melanins were much higher than those of Phanerochaete chrysosporium manganese peroxidase and lignin peroxidase.
- American Society for Microbiology
- Purification, characterization, and gene cloning of ...
Contaminated soils and sludge treatment plant - Case Study
Client: Ecosistem Group, Lamezia Terme (CZ) – Italy Challenge: to transform waste into product! The Client possesses a large plant for the treatment of products contaminated with hazardous substances and materials, recycling of recovered materials and disposal of non-recyclable waste, which is considered highly innovative and technologically advanced in the field of environmental protection. Solution: Design and production of a plant for the treatment of contaminated soils and muds by Baioni’s...
Using Electromagnetic induction to characterize soils - Case Study
Electrical conductivity measurements have been used for years to determine salinity and moisture in soils. Probes were inserted directly into the soil to determine how well the soil conducted an applied current. This process was slow and labor-intensive and was usually reserved for scientific studies. A more recent technique for measuring conductivity is electromagnetic induction (EM), a non-invasive, non-destructive sampling method. No probes are required using EM, and measurements can be done quickly and...
Defluoridation of groundwater using mixed Mukondeni clay soils
Excess fluoride in drinking water is harmful to human health and therefore it needs to be removed from water before consumption. The potential of locally available mixed Mukondeni clay soils (MMCS) as a cheap adsorbent for the removal of fluoride from aqueous solution was investigated. Characterization of MMCS was done by X-ray fluorescence, X-ray diffraction, scanning electron microscopy, Fourier transform infrared and Brunauer Emmett Teller. Cation exchange capacity and point of zero charge of the clays were...
Metagenomic approach to characterize soil microbial diversity of Phumdi at Loktak Lake
Loktak, one of the largest freshwater lakes of India, is known for floating islands (Phumdi), being made up of a heterogeneous biomass of vegetation and soil. This ecological site represents an exclusive environmental habitat wherein the rhizospheric microbial community of Phumdi plays a key role in biogeochemical cycling of nutrients. A culture-independent whole genome shotgun sequencing based metagenomic approach was employed to unravel the composition of the microbial community and its corresponding functional...
Is received dose from ingested soil independent of soil PAH concentrations: Animal model results
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