Quantitative LC-MS/MS Analysis of Microcystin Cyclopeptides: EPA Method 544

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Courtesy of Shimadzu Scientific Instruments Inc

Accurate and sensitive characterization and quantification of a structurally diverse class of microcystin cyclopeptides was accomplished using UHPLC-MS-MS. In this analysis, microcystins-RR, YR, and LR were measured using a Nexera UHPLC with a LCMS-8050 triple quadrupole MS detector with ESI ionization in accordance with EPA Method 544. The results of this study show that the Shimadzu platform is repeatable, robust, and sensitive for the measurement of microcystins in water samples using EPA Method 544. It has also been shown that the chromatography can be reduced to a method requiring 8 minutes between injections which will reduce the analysis times reported in EPA Method 544.

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