The p53 tumor suppressor pathway limits oncogenesis by inducing cell cycle arrest or apoptosis. A key p53 target gene is PUMA, which encodes a BH3-only proapoptotic protein. Here we demonstrate that Puma deletion in the Eµ-Myc mouse model of Burkitt lymphoma accelerates lymphomagenesis and that 75% of Eµ-Myc lymphomas naturally select against Puma protein expression. Furthermore, approximately 40% of primary human Burkitt lymphomas fail to express detectable levels of PUMA and in some tumors this is associated with DNA methylation. Burkitt lymphoma cell lines phenocopy the primary tumors with respect to DNA methylation and diminished PUMA expression, which can be reactivated following inhibition of DNA methyltransferases. These findings establish that PUMA is silenced in human malignancies, and they suggest PUMA as a target for the development of novel chemotherapeutics.
- American Society for Microbiology
- Selection against puma gene expression in myc-driven b-cell ...
Experiences in development and maintenance of Silence-GIS
Abstract Silence-GIS is a larse scale noise management system build as an extension on ArcGIS 9.3.1. This system has been designed and developed for the Dutch Highway Authorities. The aim of the system is to support decisions on Dutch and European noise policy and to predict the effect of future measures on the noise exposure of the population in The Netherlands. The information needed to perform noise calculations was divided over different departments. There was a great need for standardization and integration...
How to manage Silence? A large scale noise management system
AbstractSilence is the main noise management system for the Dutch highways and is used by the Dutch Ministry of Transport. The Silence system comprises IT infrastructure, environmental data, noise management software, an organisation, procedures and maintenance. Typical noise management system tasks involve data management, setup noise management software, software customisation, performing calculations and analyses and a proper maintenance cycle. In 2010 the new noise management system will become available and...
The Silence of the Lambs – Invictus Case Study
The sound of new-born lambs bleating is something that most people would be happy to wake up to on a Spring morning – or perhaps not? Noise consultant Louise Alderson, had the unusual assignment of monitoring noise levels from baby lambs and their mothers on a farm in Cumbria following a planning application to build a housing development on nearby fields – owned by the same farmer. Environmental Health Officers (EHO) from the Local Authority had raised concerns that noise from the annual spring...
Satisfying super-sensitive complainants with psychological silencing
If you play white noise to a group of people and tell them that the song “White Christmas” is hidden in the sound, about 30% of them will be convinced that they can hear it. The human mind is always looking for patterns – even when they aren’t there. This propensity explains a significant proportion of complaints where noise levels are actually very low (they can be close to, or below, the threshold of hearing at low frequencies) or the complainant has mild tinnitus. The imagined pattern...
Record breaking formula one fan noise control
Problem: an extract system fan drone creating a potential environmental noise problem. Solution: aerodynamic engineering instead of silencers – in keeping with the Red Bull Technology ethos. The conventional technique used to reduce fan tones like this is a silencer – a large, palliative silencer that doesn’t tackle the source of the problem. Not only can these reduce the system efficiency, but they may also require additional lagging and regular cleaning to maintain performance. Moreover,...