John Wiley & Sons, Ltd.

Selection of food combinations to optimize survival, growth, and reproduction of the amphipod Hyalella azteca in static‐renewal, water‐only laboratory exposures

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While standardized sediment toxicity testing methods have been developed for the amphipod Hyalella azteca, no standardized chronic water‐only toxicity testing methods have been established. Furthermore, optimal feeding and water quality conditions for culturing and toxicity testing with this species remained unclear. Our objective was to determine the food or combination of foods that best promotes survival, growth, and reproduction of the US Lab strain of Hyalella azteca under 42‐d, water‐only, static/renewal testing conditions. We conducted seven 42‐d control (no toxicant) tests with various combinations of food (including Tetramin, yeast‐cereal leaves‐trout chow (YCT), diatoms, wheat grass, alfalfa, and maple leaves) and substrate types (clean “unconditioned” nitex screens versus “conditioned” nitex screens that were colonized by live biofilms). Over all treatments, percent survival ranged from 18 to 96%, dry weight per individual from 0.084 to 1.101 mg, and reproduction from 0 to 28.4 young per female. Treatments that included Tetramin tended to result in better performance than those that did not. In particular, treatments that included Tetramin and either conditioned screens or diatoms consistently had high survival, weight, and reproduction values as well as low variability among replicates (measured as coefficient of variation). A ramped Tetramin plus diatom suspension feeding regime appears to have the greatest potential to produce consistently good performance across laboratories using static/renewal systems. This article is protected by copyright. All rights reserved

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