John Wiley & Sons, Ltd.

Selective detection and quantification of carbon nanotubes in soil

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Carbon nanotubes (CNTs) have been widely applied in many fields of industry. As the world production of CNTs is increased, the risk of the exposure of the environment to CNTs is also increased. Therefore, in order to evaluate the impact on the environment, many cell or animal toxicity experiments have been reported using CNTs. It is important to determine the degree of contamination of CNTs in soil and to find pollution pathways for the assessment of environmental toxicity of CNTs. However, the selective detection methods for CNT in soil or water are rarely reported. In the present study, a novel technique was developed to quantify the amount of CNTs in soil mixtures using fluorescent SYBR Green I dye after isolation of the CNTs using specific DNA oligomers. As a result, a limit of detection of the CNTs in soil was obtained in the range of 250 ppb. However, it will be easily extended to the level of 10 ppb using a greater capacity of magnetic well plates. This method also worked well in the presence of graphene oxide, and could be applied to the detection of CNTs in a variety of surroundings (e.g., fish and other tissues). This article is protected by copyright. All rights reserved

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