Sepacore for the Separation of Plant Extracts - Case Study

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Courtesy of BÜCHI Labortechnik AG

Customer: University of Basel, Switzerland

The Division of Pharmaceutical Biology at the University of Basel in Switzerland is a center of excellence for natural product and medicinal plant research. It belongs to the Departement of Pharmaceutical Sciences, a leading research and teaching center in drug discovery & development, drug therapy and drug safety. Research groups collaborate and teach in the areas of lead discovery, molecular mechanisms of drug action and drug toxicity, rational drug design, pharmacotherapy, and the safe use of drugs.

Application: Separation of plant extracts

The investigation of bioactive compounds contained in plants requires several steps of extraction and separation before the active substances can be obtained in a pure form for further chemical and biological characterization. In the course of this process, compounds with a broad range of polarity have to be separated.

Equipment: Sepacore®

A Sepacore System with 50 bar pumps, UV detector and fraction collector is used to separate active compounds with a broad range of polarity from plant extracts. The separation is done on prepacked silica gel (40 – 63 μm) and RP18ec (40 – 63 μm) cartridges from BUCHI.

Benefit / Conclusion:

Sepacore cartridges enable a good separation of compounds with a broad range of polarity, as typically found in plant extracts. Despite lower resolution compared to MPLC on glass columns, pre-packed cartridges are an attractive alternative for the purification of extracts and crude fractions due to their ease of use and speed of separation. Thanks to the open and modular system concept, Sepacore forms a flexible and easy to use chromatography system with a wide range of chromatography glass columns and prepacked cartridges.

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