Separation of quercetin from a Ginkgo Biloba mother tincture using the PrepChrom C-700

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Courtesy of BÜCHI Labortechnik AG

The aim of this application note was to isolate a flavonol, quercetin, from a Ginkgo Biloba mother tincture. The prepurification of the mother tincture was performed on a reversed phase flash column by the means of dry loading. The target compound was identified by its characteristic UV-VIS spectra and the retention time determined by running a standard. Subsequently, the purification of the pre-purified fraction of the target compound was performed with a 10 μm reversed phase prep-HPLC column. 

Ginkgo Biloba has been used for curative reasons for centuries. The trees nuts have been an important element in the herbal practice of traditional Chinese medicine. Due to its beauty and its botanical importance Europeans started resettling and cultivating Ginkgo Biloba in Europe in the 17th century. In the second half of the 20th century scientist started taking interest in the medical properties of Ginkgo leave extract. [1] The positive effect on many illnesses, like age-related dementia and depression could be scientifically proven. Furthermore, the enhancement of memorizing and learning functions turned the attention to the Ginkgo Biloba leave extracts. [2]

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